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Active bone peptide for treating and preventing osteoporosis and its preparing method

A technology for osteoporosis and bone peptides, applied in the field of active bone peptides for the prevention and treatment of osteoporosis and its preparation, can solve the problems of low biological activity, poor selectivity, and low yield of bone peptides, and achieve biological activity High, mild process conditions, safe and reliable products

Active Publication Date: 2006-10-11
GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation methods of osteopeptide are roughly divided into two types: acid hydrolysis and high-temperature water-soluble extraction. Their disadvantages are: poor selectivity, low biological activity, and low yield of osteopeptide

Method used

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  • Active bone peptide for treating and preventing osteoporosis and its preparing method
  • Active bone peptide for treating and preventing osteoporosis and its preparing method
  • Active bone peptide for treating and preventing osteoporosis and its preparing method

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1: Take 20 kg of fresh deer limb bones, wash the blood plasma with normal water, remove fascia and muscle, rinse 3 times with distilled water, sterilize with ultraviolet light, put it into a clean bone crusher, crush it into bone particles of 1-2 mm, add 100kg of distilled water, heated to 50°C in a constant temperature reaction tank, adjusted the pH value to 5.0 with HCl solution, added papain solution under stirring, the enzyme activity is ≥6000U / mg, stirred at 70-80r / min for 6 hours, centrifuged, and took the supernatant , placed at room temperature, placed in a refrigerator at 0-4°C for 24 hours, and degreased. Heat to 60°C for 60 minutes, concentrate in a vacuum concentrator at 50-60°C, add 6 times the volume of 95% ethanol solution to the concentrated solution, freeze for 36 hours, filter out the supernatant, and recover ethanol in a vacuum concentrator at 50-60°C until it is free of alcohol taste, dissolved in 5L sterile distilled water, centrifuged at...

Embodiment 2

[0058] Example 2: Take the limb bones of fresh sheep, cattle, pigs, horses, dogs, rabbits and other animals respectively, and prepare active bone peptides according to the method of the above-mentioned Example 1. After cell culture and animal experiments, it also has a certain effect on promoting bone formation. It can be used to prevent and treat osteoporosis.

Embodiment 3

[0059] Embodiment three: get the limb bones of fresh sheep, cattle, pigs, horses, dogs, rabbits and other animals respectively, and use pepsin, trypsin, chymotrypsin or subtilisin instead of papaya according to the method of above-mentioned embodiment one The active bone peptide prepared by enzymatic hydrolysis with protease has a good effect on promoting bone formation through cell culture and animal experiments, and can also be used to prevent and treat osteoporosis.

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Abstract

The invention relates to a active bone peptide for treating and preventing osteoporosis and process for its preparation, which comprises the following steps: (1) cleaning bones of animal limbs, sterilizing, disintegrating, charging distilled water, controlling hydrolysis with proteinase, filtering the supernatant fluid, (2) degreasing the supernatant fluid, concentrating, ethanol extracting, filtering the supernatant fluid, (3) dealcoholizing the supernatant fluid, centrifugal filtrating, gathering the filtering medium, (4) making the active bone peptide into any one of the pharmacological dosage forms.

Description

technical field [0001] The invention belongs to the fields of traditional Chinese medicine, biochemical medicine and health food, and specifically relates to an active bone peptide for preventing and treating osteoporosis and a preparation method thereof Background technique [0002] There are more than 90 million people suffering from osteoporosis in my country, accounting for 7.1% of the total population, and it will reach 220 million in 2050. With the aging of the world's population, the number of people suffering from osteoporosis and osteopenia is increasing. Facing the huge population of osteoporosis patients, the demand for drugs is increasing year by year. From an economic point of view, research and development Anti-osteoporosis drugs, especially new drugs and health foods with independent intellectual property rights, have important economic and social benefits for improving human health and quality of life. [0003] At present, there are three types of drugs for ...

Claims

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Application Information

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IPC IPC(8): A61K38/01A61K35/32A61K9/00A61P19/10A61P19/08
Inventor 李延平崔燎邓亦峰吴科锋艾春媚刘义黄连芳
Owner GUANGDONG MEDICAL UNIV
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