Combined test kit for bacterial vagina disease

A vaginosis and kit technology, applied in the field of bacterial vaginosis combined assay kits, can solve the problems of poor specificity, inconvenience for users, inconvenience for universal use, etc., and achieves the effects of no equipment, low cost, and comprehensive detection.

Inactive Publication Date: 2006-10-25
北京中生金域诊断技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(2) The operation of microbiological inspection is troublesome and has been rarely used
However, the specificity of single enzyme activity determination in diagnosing BV is relatively poor. Determination of trimethylamine, sialidase + hydrogen peroxide, etc.
Considering the commercial cost, most of these products are sold as a single item, which is inconvenient for users and high in analysis cost. For example, the cost of materials for the determination of sialidase and leukocyte esterase by foreign BV Blue and Leakostix is ​​as high as 40 Renminbi, inconvenient to be widely used

Method used

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  • Combined test kit for bacterial vagina disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] In the three holes of the dry chemical reaction device of embodiment 1, the 3MM grade chromatographic paper of Whatman of phi 4.5mm is equipped with respectively the hydrogen peroxide index pad of carrier, leukocyte esterase index pad and sialidase activity reagent pad;

[0035]Production of hydrogen peroxide indicator pad: soak the above-mentioned carrier in the following immersion solution; the immersion solution is prepared by peroxidase 1.2U / m 2 , pH=4.6, 100mmol / L sodium phosphate buffer, 4-aminoantipyridine 5g / m 2 , 3,5 dichlorodihydroxybenzenesulfonic acid 6.7g / m 2 and Vitamin C 2.7g / m 2 composition;

[0036] Production of leukocyte esterase index pad: soak the above carrier in the following immersion solution; the immersion solution is composed of 5 bromo-4 chloro-3 indole acetate 2 and sucrose 15% composition;

[0037] Production of sialidase activity reagent pad: 5 bromo-4 chloro-3 indole neuraminic acid salt 138mg / m 2 , pH=5.2, 200mmol / L sodium acetate b...

Embodiment 2

[0047] In the three holes of the dry chemical reaction device of embodiment 2, the 3MM grade chromatographic paper of Whatman of φ 4.5mm is equipped with respectively the hydrogen peroxide index pad of carrier, leukocyte esterase index pad and sialidase activity reagent pad;

[0048] Production of hydrogen peroxide indicator pad: soak the above-mentioned carrier in the following immersion solution; the immersion solution is prepared by peroxidase 1.8U / m 2 , pH=4.6, 100mmol / L potassium phosphate buffer, 4-aminoantipyridine 7.5g / m 2 , 3,5 dichlorodihydroxybenzenesulfonic acid 10.05g / m 2 , vitamin C 2.7g / m 2 ;

[0049] Production of white blood cell esterase indicator pad: soak the above carrier in the following immersion solution; the immersion solution is composed of 5 bromo-4 chloro-3 indole acetate 2 , sucrose 12%;

[0050] Preparation of sialidase activity reagent pad: soak the above-mentioned carrier in the following immersion solution; 2 , pH=5.2, 200mmol / L potassium ...

Embodiment 3

[0055] In the three holes of the dry chemical reaction device of embodiment 3, the 3MM grade chromatographic paper of Whatman of phi 4.5mm is equipped with respectively the hydrogen peroxide index pad of carrier, leukocyte esterase index pad and sialidase activity reagent pad;

[0056] Production of hydrogen peroxide indicator pad: soak the above-mentioned carrier in the following immersion solution; the immersion solution is prepared by peroxidase 1.8U / m 2 , pH=4.6, 100mmol / L sodium phosphate buffer, 4-aminoantipyridine 8.35g / m 2 , 3,5 dichlorodihydroxybenzenesulfonic acid 11.1g / m 2 , vitamin C 2.7g / m 2 .

[0057] Production of white blood cell esterase indicator pad: soak the above carrier in the following immersion solution; the immersion solution is composed of 5 bromo-4 chloro-3 indole acetate 2 , Trehalose 10%.

[0058] Preparation of sialidase activity reagent pad: soak the above-mentioned carrier in the following immersion solution; the immersion solution is compos...

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Abstract

The invention provides the reagent box checking the bacterial vaginal disease, it includes the dry chemic reacting setting, the diluent and the liquid showing the color; the reacting setting has three blind holes placed the reagent mat testing the chroma of excessive oxidation hydrogen, the chroma of the leucocyte ester enzyme and the active ability of the saliva acid enzyme; the regent mat testing the chroma of the excessive oxidation hydrogen is dipped in the enzyme solution containing the peroxide enzyme, the phosphate amoritizing liquid, the 4-amino antipyrine, the 3,5-double chlorine double hydroxide group dobesilate and the vitamin C and dried. The reagent mat testing the chroma of the leucocyte ester enzyme is dipped in the enzyme solution containing the 5 bromine-4 chlorin- 3 heteroauxing salt and the cane suger and is dried; the reagent mat testing the active ability of the saliva acid enzyme is dipped in the 5 bromine-4 chlorin-3 indloe nerve arginine salt, the acetic acid salt cushioning liquid and the fucose and is dried; the cushioning liquid is composed of the physiological brine and MES cushioning liquid; the liquid showing the color is the B solution fixing the purple. The entironment, the cleanliness of the procreating path of the checked and the condition of the bug inducing the bacterial vagina can be test at the same time; it has many merits such as the easy handling, the celerity and the cheap cost.

Description

technical field [0001] The invention belongs to the field of in vitro diagnostic equipment, and specially designs a bacterial vaginosis joint detection kit, which is used for evaluating the micro-ecological environment, cleanliness and bacterial vaginosis pathogenic bacteria status of women's lower reproductive tract. Background technique [0002] Bacterial vaginosis (Bactarial Vaginosis, referred to as BV) is a bacteriological manifestation of a significant reduction in the number of normal vaginal flora, replaced by a group of anaerobic flora (Bacteroides, Mobilenian anaerobes, and Dana bacilli, Peptostreptococcus and human Mycoplasma, etc.) increased significantly, and the biochemical properties of vaginal fluid changed significantly and other clinical symptoms caused by changes in vaginal microecology. [0003] The diagnosis of bacterial vaginosis generally includes three categories: clinical diagnosis, microbiological diagnosis and vaginal fluid biochemical diagnosis. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/28
Inventor 王加义
Owner 北京中生金域诊断技术股份有限公司
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