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Sulfa drug multi-residual conjoined detection test paper box

A technology of test paper box and sulfa drug, which is applied in the field of rapid detection of trace amount of sulfa drug, can solve the problems of complex operation process, limited application range, long time required, etc., to improve work efficiency, wide application range, and cost saving Effect

Inactive Publication Date: 2007-01-03
河南省动物免疫学重点实验室
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some of these methods require expensive instruments and equipment, require skilled professionals to operate, the operation process is complicated, and it takes a long time, which limits its application range and is difficult to promote and apply in actual production.

Method used

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  • Sulfa drug multi-residual conjoined detection test paper box
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  • Sulfa drug multi-residual conjoined detection test paper box

Examples

Experimental program
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Effect test

preparation example Construction

[0045] 3. Preparation of gold standard binding pad

[0046] ①Preparation of gold-labeled antibody binding pad: Prepare colloidal gold solution by sodium citrate reduction method, that is, add 8ml of freshly prepared 1% sodium citrate to 200ml of boiling 0.01-0.02% chloroauric acid solution to obtain a colloidal gold solution with a diameter of about 15nm. Colloidal gold solution, with 0.1mol / L K 2 CO 3 Adjust the pH to 8.5-9.5, and store at 2-8°C for later use. Add the monoclonal antibody or polyclonal antibody to be labeled into the gold sol with a pH of 8.5-9.5 at a labeling ratio of 1:2000. After labeling for 10 minutes, add 20% PEG10000 to a final concentration of 0.05%. Centrifuge at 2000rpm for 20min to remove unbound colloidal gold particles, centrifuge at 15000rpm at 4°C for 1h, discard the supernatant to obtain the initially purified gold-labeled antibody protein, and then use propylene dextran S-400 column chromatography to separate and purify to obtain the colloid...

Embodiment 1

[0058] Example 1: Gold Standard Chromatography Test Kit for Two Items of Joint Inspection of Sulfonamide Drugs

[0059] The structure of the plastic shell of the test paper box includes a base (11) and a panel (10). The base is provided with a fixing groove (12) for installing the test paper core. The panel is provided with a sample injection hole (8) and a display window (9). with T 1 , T 2 and C marked. The structure of the test paper core is: the liner (1) is made of hard plastic strips; the sample pad (2) is made of glass fiber cotton; The preparation method of preparing the anti-SDM and anti-SM with colloidal gold label 2 The glass fiber cotton of monoclonal antibody; Reaction membrane (4) uses nitrocellulose membrane, is used to couple SDM-carrier protein and SM respectively on it 2 - Carrier protein combined with antigen to prepare invisible detection line "|", arranged sequentially from the sample side and connected with the T on the panel 1 , T 2 Correspondingly...

Embodiment 2

[0067] Example 2: Gold Standard Chromatography Test Kit for Two Items of Joint Inspection of Sulfonamide Drugs

[0068] The structure of the test paper box is basically the same as that of Example 1, the difference is that anti-SDM and anti-SM are used 2 Polyclonal Antibody Replacement Anti-SDM and Anti-SM 2 The monoclonal antibody was used to prepare the gold-labeled binding pad; the goat anti-rabbit IgG antibody was used instead of the rabbit anti-mouse IgG antibody to prepare the invisible control line "|" on the reaction membrane.

[0069] Others including test sample preparation, operation method and result judgment are the same as in Example 1, and will not be repeated.

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Abstract

A gold-linked chromatography test paper box uses for analysis of the sulfonamides excessive remains. The test paper box makes up by plastic shell and test paper core, and the faceplate has sample adding hole and result displaying window. The reaction film is pyroxylin film with recessive test line and comparison line. Gold-linked combine pillow is fiberglass cotton with 2-5 strips mixed gold-linked antibody and the test line of the reaction film is 2-5 strips opposite sulfonamides-carrier albumen unite antigen. Or Gold-linked combine pillow is fiberglass cotton with 2-5 strips gold-linked sulfonamides-carrier albumen unite antigen, while the test line of the reaction film is 2-5 strips corresponding antibody to some sulfonamides. The comparison line of the reaction film is goat antirat or rabbit antirat IgG, or goat anti rabbit IgG, or rabbit-anti carrier albumen IgG. The box can test manifold sulfonamides at the same time and greatly save the test time with strong character, high sensitive, easy, intuitive, nicety and wide applied range, low cost for extending.

Description

[0001] 1. Technical field: the present invention relates to a device for rapidly detecting trace amounts of sulfonamides, in particular to a gold standard chromatography detection test kit for multi-residue analysis of sulfonamides. 2. Technical background: [0002] Sulfa drugs are a class of chemical drugs used to prevent and treat bacterial infectious diseases. It has the characteristics of wide antibacterial spectrum, low price, and convenient use. In addition to being widely used in veterinary clinical treatment, it is often used as a feed additive in animal production to control certain diseases. Due to the non-standard use of sulfa drugs in animal production, they remain in the animal body, and enter the human body through the food chain such as meat, eggs, milk, etc., and accumulate in the human body, causing many bacteria to develop drug resistance, causing allergic reactions, and poisoning , can also cause hematopoietic system disorders, acute hemolytic anemia, agranu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558G01N33/544G01N33/52
Inventor 邓瑞广张改平李学伍肖治军刑广旭杨继飞柴书军李青梅卢晓翠
Owner 河南省动物免疫学重点实验室
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