Acerola leaf extract-containing blood sugar level increase inhibitor and age formation inhibitor, and food containing them
A blood sugar level and inhibitor technology, which is applied in the direction of food ingredients containing natural extracts, medical preparations containing active ingredients, food preparation, etc., can solve the problem that the useful components of the fruit and leaves of the yellow mattress have not been detected.
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Embodiment 1
[0048] After uniformly processing the leaves of Cassiopsis chinensis, 3 times the volume of distilled water was added thereto, followed by extraction for 1 hour. The above steps were carried out twice, and the extract was centrifuged, filtered, freeze-dried, and then diluted in distilled water again. The resulting solution was packed on a C18 column (Sep-Pak Vac 35cc C18 column, Waters), washed with distilled water, and eluted with 0.2% TFA / methanol solution. The eluted fractions were evaporated to dryness to obtain a purified extract.
[0049] Next, the polyphenol content in the resulting purified extract was measured by the Folin-Denis method. Specifically, the purified extract was dissolved in distilled water at a concentration of 0.5 mg / ml, and 0.1 ml of the resulting solution, 2.9 ml of distilled water, and 0.5 ml of Folin-Ciocalteu reagent (Merck) were mixed. The mixture was left for 3 minutes, 20% sodium carbonate solution was added thereto, the mixture was left for 6...
Embodiment 2
[0052] According to the following method, the α-glucosidase inhibitory activity (maltase inhibitory activity) was measured for the purified C. japonica fruit leaf extract prepared in Example 1.
[0053] To commercially available rat small intestine acetone powder, 9 times the volume of 56 mM maleate buffer (pH 6.0) was added, and the mixture was homogenized with a glass homogenizer. After the product was centrifuged, the supernatant was recovered and defined as the crude enzyme solution. A 20-fold dilution of this crude enzyme solution was used in the maltase reaction.
[0054] Initially, 0.6ml of 2mg / ml sample solution was added to 0.6ml of 2% maltose solution, the mixture was incubated at 37°C for 5 minutes, 0.6ml of the above crude enzyme solution was added thereto, and the reaction was carried out at 37°C for 120 minutes. The enzyme was inactivated by heating in boiling water for 10 minutes, and after centrifugation, the glucose level in the supernatant was measured by HP...
Embodiment 3
[0065] According to the following method, the inhibitory activity against AGE production was measured on the purified C. chinensis fruit leaf extract prepared in Example 1.
[0066] Bovine serum albumin (16 mg / ml, 1 ml), 1 ml of 4M glucose, 1 ml of 1 / 15M phosphate buffer (pH 7.2), and 1 ml of 0.3 mg / ml sample solution were mixed, and the product was stored at 60°C. Seven days later, AGEs generated from proteins and glucose were measured using a spectrofluorometer. AGE primary products were analyzed using an excitation wavelength of 325 nm and an emission wavelength of 405 nm. AGE higher order products were analyzed using an excitation wavelength of 370 nm and an emission wavelength of 440 nm. For control purposes, experiments with aminoguanidine were performed in the same manner. In addition, the same experiment as above was repeated except that distilled water was used instead of the sample solution (the same amount as that used for the sample solution), and the reaction pr...
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