Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Paddy disease-resistance gene OsDR6

A rice and genetic technology, applied in the field of genetic engineering, can solve problems such as yield and quality decline

Inactive Publication Date: 2007-04-25
HUAZHONG AGRI UNIV
View PDF1 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Rice is an important food crop in the world, but the impact of diseases often leads to the decline of its yield and quality

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Paddy disease-resistance gene OsDR6
  • Paddy disease-resistance gene OsDR6
  • Paddy disease-resistance gene OsDR6

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Analysis of the expression pattern of OsDR6 gene in rice varieties

[0022] The gene encoding AtMPK4 in Arabidopsis is a negative regulatory gene in disease resistance response (Asai et al., 2002, MAP kinase signaling cascade in Arabidopsis innate immunity, Nature, 415: 977-983). In order to detect whether the homologous gene of AtMPK4 gene in rice is also a negative regulator of disease resistance response, we used BLASTP analysis method (Altschul et al., 1997, Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res.25: 3389-3402), using the amino acid sequence encoded by the AtMPK4 gene to search the EST (expressed sequence tag) sequence database of the rice variety Minghui 63 (Zhang et al., 2005, Features of the expressed sequences revealed by a large-scale analysis of ESTs from a normalized cDNA library of the eliteindica rice cultivar Minghui 63.Plant J.42: 772-780), it was found that the coding sequence of the...

Embodiment 2

[0026] Example 2: Determining the genome sequence and gene structure analysis of the OsDR6 gene

[0027] 1. Prediction of OsDR6 gene structure

[0028] Using the cDNA sequence EI106L06 of the OsDR6 gene as a template, the nucleotide database was searched by BLAST method, and a 300029bp sequence located on the rice chromosome 10 from the rice variety Nipponbare was found in the database (the nucleotide database GenBank (http: / / www. ncbi.nlm.nih.gov) registration number: AE017115) has a sequence (55596 to 59326bp) homology with EI106L06 reaches 100% (E value = 0), suggesting that AE017115 contains genes homologous to OsDR6, That is, the allele of OsDR6.

[0029] Using GENSCAN (http: / / genes.mit.edu / GENSCAN.html), GeneFinder (http: / / genomic.sanger.ac.uk / gf / gf.shtml), Gene Feature Searches (http: / / dot.imgen .bem.tmc.edu:9331 / ), GeneMark (http: / / genemark.biology.gatech.edu / GeneMark / hmmchoice.html) and other gene structure prediction software analysis and prediction of homologous g...

Embodiment 3

[0034] Example 3: Analysis of OsDR6 Gene Encoding Product

[0035] According to the results of BLAST analysis, the protein encoded by the OsDR6 gene is the same as the encoded product of the Arabidopsis AtMPK4 gene (National Center for Biotechnology Information (NCBI, http: / / www.ncbi.nlm.nih.gov) protein database registration number: S40470). source, the amino acid identity is 84% ​​(E value=e-180), and the amino acid identity with the coding product of Arabidopsis AtMPK5 (NCBI protein database registration number: S40471) is 74% (E value=e-161), The amino acid identity with corn salt-induced SIMK1 (salt-induced MAP kinase 1) protein (NCBI protein database registration number: AAR11450.1) is 93% (E value = 0.0). Because the above-mentioned Arabidopsis and maize genes both encode cytokine-activated protein kinases, the encoded product of the OsDR6 gene is most likely a cytokine-activated protein kinase

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of plant biotechnology. The invention specifically involves the separation and cloning of a DNA fragment of rice anti-diseases related gene OsDR6 and functional verification. Combining part of the DNA fragment of OsDR6 gene with vectors able to express double-stranded RNA and transforming into diseased rice varieties using transgenic technology based on RNA interference (RNAi) principle to inhibit the expression of OsDR6 in diseased rice varieties. Resistance of the genetic transformed rice with OsDR6 gene expression significantly reducing against prostheca blight bacteria increases apparently, proving that gene OsDR6 is negative regulation factor in bacterial leaf blight resistant reaction of the rice. The disease-resistant capacity of the rice can be enhanced by inhibiting its function.

Description

technical field [0001] The invention relates to the technical field of genetic engineering. It specifically involves the isolation, cloning and functional verification of a rice disease resistance-related gene OsDR6. OsDR6 gene is a negative regulator in rice disease resistance response. Inhibiting the function of the OsDR6 gene can significantly enhance the ability of rice to resist diseases. Background technique [0002] Plants are attacked by various pathogens during their growth. There are many types of plant pathogens, including viruses, bacteria, fungi, and nematodes. Pathogen invasion of plants leads to two results: (1) the pathogen successfully reproduces in the host plant, causing related diseases; (2) the host plant produces a disease-resistant response, killing the pathogen or preventing its growth. Using resistance gene resources to improve plant disease resistance is the fundamental way to prevent diseases and protect the environment at the same time. [00...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82
Inventor 王石平袁斌付晶
Owner HUAZHONG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com