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Application of total flavones of chickpea in preparation of medicament for treating diabetes

A technology of diabetes medicine and total flavonoids, which is applied in the application field of chickpea total flavonoids in the preparation of diabetes medicines, can solve the problems of low flavonoid content and no medicinal value, and achieve the effect of low cost and simple extraction process

Inactive Publication Date: 2007-05-23
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the crude product of chickpea alcohol extract has low flavonoid content and has no medicinal value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Step 1: Put 200g of chickpea powder crushed to 0.5-5mm in a round bottom flask, add 1000ml of petroleum ether, heat to a slight boil and reflux for 1h, filter and collect the chickpea powder, add 1000ml of petroleum ether and reflux for 1h, Repeat a total of three times. Dry the obtained defatted chickpea powder and add 2400ml of 80% ethanol solution, reflux three times at 65° C. for 3 hours each time, filter, rotary evaporate, recover ethanol, and obtain dark brown thick paste.

[0020] Step 2: Dissolve the obtained thick paste with hot water, adjust the pH value to 10, centrifuge the obtained solution, and take the supernatant. Put the obtained solution on a D101 macroporous resin column, and use a 20% to 60% ethanol aqueous solution for gradient elution, collect 60% of the eluate, and rotary evaporate to obtain a yellow-green powder. The sample is detected by HPLC, and the flavonoid content is 54.3%. .

Embodiment 2

[0022] Step 1: Put 200g of chickpea powder crushed to 0.5-5mm in a round bottom flask, add 1000ml of petroleum ether, heat to a slight boil and reflux for 1h, collect the chickpea powder by suction filtration, add 1000ml of petroleum ether and reflux for 1h, repeat three times. The obtained defatted chickpea powder was dried, added 2400ml of 80% ethanol solution, refluxed at 70°C for 3 times, each time for 3 hours, filtered, rotary evaporated, recovered ethanol, and obtained dark brown thick paste.

[0023] Step 2: Dissolve the obtained thick paste with hot water, adjust the pH value to 10, centrifuge the obtained solution, and take the supernatant. Put the obtained solution on a DS-401 macroporous resin column, and use 20% to 60% ethanol aqueous solution gradient elution, collect 50% of the eluent, and rotary evaporate to obtain a yellow-green powder. The sample is detected by HPLC, and the flavonoid content is 53.2%.

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PUM

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Abstract

The invention relates to an application of chick-pea flavone in antidiabetic, wherein said production comprises that: using ligarine to degrease chick-pea, drying, using alcohol to extract, dissolving the paste in hot water, adjusting pH to 9-11, eccentrically filtering, taking out upper clear liquid, separating said clear liquid via porous resin post, using 20-60% alcohol solution to wash, collecting the part with high flavone content, evaporating solvent, to obtain the final product whose flavone content is higher than 50%. The inventive method has simple process and low cost.

Description

technical field [0001] The invention relates to total chickpea flavonoids and its extraction method and application in medicine, in particular to total chickpea flavonoids and its extraction method and its application in the preparation of medicines for treating diabetes. Background technique [0002] Diabetes mellitus is a metabolic disease with multiple etiologies, characterized by chronic hyperglycemia, accompanied by disturbances in sugar, fat and protein metabolism caused by defects in insulin secretion and / or action. With the prolongation of diabetes, if the metabolic disorder in the body is not well controlled, it will lead to blindness, cardiovascular and cerebrovascular diseases, renal failure, neuropathy, coma and other complications, and even death. In terms of incidence, the actual number of diabetic patients in my country has reached more than 40 million, and the incidence is increasing at an alarming rate. There are more than 3,000 new diabetic patients every d...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61K9/14A61K9/16A61K9/20A61K9/48A61P3/10C07D311/30A61K31/352
Inventor 孙晓莉王四旺刘雪英邢江浩
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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