Method for fast determination of medicament bacteriostatic activity of rice false smut

A rice smut fungus and antibacterial activity technology, which is applied in the field of rapid determination of the antibacterial activity of medicaments on rice smut fungus, can solve the problems of large error, long cycle, slow speed, etc. effect of sensitivity

Inactive Publication Date: 2010-05-19
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for measuring the bacteriostatic activity of the rice smut bacterium against the problems of slow speed, long cycle, heavy pollution, and large errors when the conventional growth rate determination method is used for the determination of the biological activity of the rice smut bacterium. Fast, efficient and accurate evaluation method

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  • Method for fast determination of medicament bacteriostatic activity of rice false smut

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Embodiment 1

[0020] Embodiment 1: (a kind of rapid assay method that rice false smut is carried out medicament antibacterial activity)

[0021] Specifically follow the steps below:

[0022] (1) Preparation of medium: According to the growth rate of rice smut bacteria in the medium suitable for fungal culture, the YPPSA medium used for the determination of the antibacterial activity of rice smut bacteria agents was screened. Wherein, the culture medium (YPPSA) of plate culture: be potato 150g, sucrose 10g, yeast powder 100mg, peptone 100mg, agar 18g, pH value 6.5, 1000ml distilled water, 121 ℃, form by autoclaving; YPPS): 150g potatoes, 10g sucrose, 100mg yeast powder, 100mg peptone, pH 6.5, 1000ml distilled water, 50ml aliquots, sterilized under high pressure at 121°C;

[0023] (2) Activation culture of the rice false smut strain: pick a little mycelia block of the preserved rice false smut bacteria into the YPPSA plate culture medium, and culture in the dark at a constant temperature of ...

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Abstract

The invention discloses a rapid detection method of agentia bacteriostasis activity against green smut bacterial, and belongs to the field of biological detection of fungous pathogenic bacteria resistant agentia. The method comprises: (1) preparing the medium, (2) activating the strain and culturing, (3) culturing green smut bacteria seed solution, (4) preparing agentia culture solution to be detected, (5) detecting the culture and growth of green smut bacteria in the culture solution, and (6) calculating the agentia bacteriostasis activity. The invention is designed according to the characteristics of green smut bacteria that it is grows slowly and the mycelial grows overlay and heaped-up. Compared with the general plate bacteriostasis method, the invention shortens the detection period from 4-6 weeks to 2 weeks, improves the sensitivity and accuracy of green smut bacterial against agentia, and reduces the polluting possibility during detection and detection cost. The invention can beused in the field of biological detection of fungous pathogenic bacteria resistant agentia.

Description

technical field [0001] The invention relates to the technical field of bioassay of fungal agents, in particular to a new method for rapidly measuring the antibacterial activity of agents against rice smut. Background technique [0002] Rice false smut (Ustilaginoidea virens (cooke) Tak) is a worldwide fungal disease, belonging to Ascomycetes Ergotaceae, also known as pseudo smut, black ball and surface powdery disease, and because of rice false smut often occurs in rice Harvest years are so serious that they are called "harvest diseases". In recent years, with the promotion of susceptible varieties such as hybrid japonica rice and the improvement of soil fertility, the occurrence area and incidence of rice false smut have increased, and even caused serious economic losses in some areas. Several pesticides, such as Jinggangmycin, which have been used to control rice smut for a long time in production, are not very effective due to the increase in drug resistance, and copper ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04C12R1/645
Inventor 孙国昌张震杜新法柴荣耀王艳丽毛雪琴邱海萍王教瑜
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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