Bacterial spore compositions for industrial uses

a technology of spores and compositions, applied in the field of bacteria spore compositions, can solve the problems of inadequate shelf life, inability to form i>bacillus /i>species and other spore forming bacteria in their vegetative form, and inability to meet the harsh conditions of industrial use, so as to reduce mortality, increase weight gain, and reduce the number of lesion

Inactive Publication Date: 2016-02-11
ENVERA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0046]In another aspect, the present invention relates to a method of treating or preventing a disease in a subject susceptible to or afflicted with the disease comprising administering to the subject an effective amount of a composition comprising a bacterial spore and a germinative compound, thereby treating or preventing the disease in the subject. In certain embodiments, the disease is a bacterial disease, a fungal disease or a viral disease. In certain embodiments, the disease is necrotic enteritis. In certain embodiments, the subject is a chicken, a turkey or a duck. In certain embodiments, the subject is infected with Clostridium perfringens. In certain embodiments, treating or preventing the disease results in one or more of reducing mortality, reducing lesion number, and increasing weight gain compared to a subject that is not administered the composition.

Problems solved by technology

One difficulty associated with the use of such bacteria is that while they must be in their vegetative state to afford such benefits, it is difficult to formulate Bacillus species and other spore forming bacteria in their vegetative form such that they will possess an adequate shelf life.
In addition Bacillus species in their vegetative form may not be able to survive the harsh conditions needed for industrial uses, such as the conditions to which seeds or other plant propagative materials are subjected before conditions suitable for seed germination occur.
However one of the challenges in using combinations of bacterial spores and germinative compounds in industrial uses is maintaining the spore in an inactive form in the presence of the germinative compound until germination of the spores is required.
In addition, bacteria for agricultural or industrial use may be exposed to a range of environmental conditions such as low soil pH, high salt concentrations (e.g. NaCl), and high metal concentrations (e.g. copper and aluminum) that are unfavorable for bacterial spore germination.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Increased Germination of B. subtilis ENV 923 in an Intimate Mixture with L-alanine

[0158]In order to compare the germination rate of the spores of intimate mixtures this invention with that of spores conventionally employed in the absence of a germinant, the following treatments were performed:[0159]A. Formation of an intimate mixture: Spores of B. subtilis ENV 923 were spray dried with L-alanine being introduced to the spore mass immediately prior to spray drying as a solution containing 0.044 grams of alanine per milliliter of distilled water. The intimate mixture produced was germinated by subsequent introduction in a solution consisting of 0.01 M phosphate buffer in distilled water with resultant pH 7.[0160]B. Conventional mixing of spores with a germinant: Spores of B. subtilis ENV 923 were spray dried and subsequently introduced into a solution consisting of 0.01 M phosphate buffer in distilled water with resultant pH 7. Such spores were germinated by the introduction in a sol...

example 2

Increased Germination of Bacillus subtilis Strain ENV923 Treated with GOSD

[0164]Bacillus subtilis spores were treated with GOSD (Germination Optimized Spray Drying) via spray drying spores in the presence of a solution of L-alanine, and germination levels were determined via Optical Density (OD) readings.

0.01 M Potassium Phosphate Buffer, pH 7

[0165]The 0.01M Potassium Phosphate buffer was prepared using 1M K2HPO4 (87.09 g dissolved in 0.5 L distilled water) and 1M KH2PO4 (68.045 g dissolved in 0.5 L distilled water) solutions. Combining 61.5 ml of 1M K2HPO4 with 38.5 ml 1 M KH2PO4 and diluting to 1000 ml with distilled water 0.1M Potassium Phosphate buffer at pH 7.0 was made. Further diluting the 0.1M Potassium Phosphate buffer with distilled water at the ration 1:10, the 0.01M Potassium Phosphate buffer, pH 7.0 was obtained. The buffer was sterilized by autoclaving at 121° C. for sixty (60) minutes.

[0166]Bacillus subtilis spore suspensions were prepared at the concentration 1.7×108...

example 3

Increased Germination of Bacillus licheniformis Strain ENV100 Treated with GOSD

[0167]Bacillus licheniformis spores were treated with GOSD via spray drying spores in the presence of 0.044 grams of L-alanine per mL of distilled water as described in Example 1. Germination levels were determined via Optical Density (OD) readings as described above using 0.01 M Potassium Phosphate buffer, pH 7.0 for spore suspension preparation.

[0168]Bacillus licheniformis spore suspensions were prepared at the concentration 1.29×108 cfu / ml in 0.01 M Potassium Phosphate buffer, pH 7.0, incubated in the preheated 37° C. water bath, and evaluated for percent of germination in five (5) minute intervals over a forty five (45) minute period.

TABLE 3% Germination of Bacillus licheniformis treated and non-treated spore suspensionsMinutes051015202530354045B. licheniformis, control01%5%3%10%11%10%10%10%10%B. licheniformis, GOSD04%5%39%60%81%85%88%91%91%Conclusion: GOSD treatment significantly enhanced percent ger...

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Abstract

In one aspect, the present invention is directed to a plant or plant part coated with a composition comprising a bacterial spore and a germinative compound. In another aspect the present invention is directed to a method of enhancing the growth of a plant or plant part comprising coating such plant or plant part with such a composition. In yet another aspect, the present invention is directed to a composition comprising a bacterial spore and a germinative compound where such components are maintained in an inactive form. The present invention also relates to use of the compositions in wastewater treatment, environmental remediation, oil recovery, aquaculture systems, and direct fed microbials.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application claims the priority benefit of U.S. Provisional Application Ser. No. 62 / 033,825 filed Aug. 6, 2014, which is hereby incorporated by reference in its entirety.FIELD OF THE INVENTION[0002]The present invention relates generally to bacterial spore compositions for industrial uses such as agriculture, environmental remediation, composting, methane production, cleaning supplies, oil recovery, and direct fed microbials.BACKGROUND OF THE INVENTION[0003]Spore-forming bacterial species have a wide range of industrial applications including uses in agriculture, environmental remediation, composting, methane production, oil recovery, and cleaning supplies. Depending upon the particular bacterial species employed, compositions comprising the bacteria may be suitable for agricultural, horticultural, environmental, probiotic, aquatic, industrial and sanitation uses, among others. Several spore-forming bacteria species have utility acro...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01N25/00A01N63/22A01N63/28C05F11/08
CPCA01N63/00A01N25/00C05F11/08C02F3/34C02F11/04B09C1/10A23K10/12A23K10/18A23K50/75A23K50/80A61K2035/115Y02A40/818A61K35/742B09C1/105B09C2101/00C02F3/348C09K8/582C11D3/381C12N1/20C12P5/023A61P1/00A61P1/04A61P3/02A61P31/04A61P31/10A61P31/12Y02W10/20Y02W30/40A01N63/22A01N63/28Y02E50/30A01N37/44A01N37/46A01N43/16A01N59/00A61K47/12C11D3/221C11D3/33
Inventor HASHMAN, TOMMIE, EUGENE
Owner ENVERA
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