Prevention and reversal of inflammation induced DNA damage

a dna damage and inflammation technology, applied in the field of prevention and reversal of inflammation-induced dna damage, can solve the problems of the elimination of global antioxidant treatment effect, and achieve the effects of reducing neuronal sensitivity, reducing inflammation and chronic pain, and enhancing the dna base excision repair pathway

Inactive Publication Date: 2019-04-25
INDIANA UNIV RES & TECH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0009]The present disclosure relates generally to methods of reducing neuronal sensitivity, thereby reducing inflammatory and chronic pain. Particularly, it has been found herein that by enhancing the DNA base excision repair (BER) pathway, through the administration of APX3330 (and / or analogs thereof), neuronal sensitivity to inflammatory mediators is reduced, thereby alleviating inflammatory or chronic pain.

Problems solved by technology

In particular, several studies have suggested a reversal of neuronal sensitivity with antioxidants (Khattab, 2006, Keeble et al., 2009, Fidanboylu et al., 2011, Duggett et al., 2016), yet there are deleterious effects of global antioxidant treatment due to the ubiquitous role of ROS / RNS in cellular signaling and cellular redox homeostasis (see Martin and Barrett, 2002).
As current therapies have limited efficacy and can result in significant side effects, determining the mechanisms for maintaining peripheral sensitization and using that information to design new therapies for treating inflammatory and chronic pain is clinically significant.

Method used

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  • Prevention and reversal of inflammation induced DNA damage
  • Prevention and reversal of inflammation induced DNA damage
  • Prevention and reversal of inflammation induced DNA damage

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0042]In this Example, the dependency of persistent changes in the sensitivity of sensory neurons secondary to exposure to inflammatory mediates on DNA damage was analyzed. Further, the effects of enhancing the DNA BER pathway on DNA damage and neuronal sensitivity were analyzed.

[0043]Materials and Methods

[0044]Unless otherwise specified, tissue culture supplies were obtained from Thermo Fisher Scientific (Waltham, Mass.). Poly-D-lysine, laminin, mouse monoclonal anti-vinculin antibody, 1-methyl-2-pyrrolidone (MPL), complete Freund's adjuvant (CFA), and routine chemicals were purchased from Sigma-Aldrich (St. Louis, Mo.). Nerve growth factor was purchased from Envigo (Indianapolis, Ind.) and Normocin from Invivogen (San Diego, Calif.). Neuroporter was purchased from Genlantis (San Diego, Calif.). Mouse monoclonal antihuman APE1 antibodies were raised in the laboratory and available from Novus Biologicals (Littleton, Colo.), mouse monoclonal anti-phospho-H2AX antibody was from EMD Mi...

example 2

[0082]In this Example, APX3330 was analyzed for its effects on DNA repair activity.

[0083]Neuroblastoma cells were implanted subcutaneously into the right flanks of 6-wk old male NSG mice and allowed to proliferate until tumor volumes ≥150 mm3. Mice were then randomized for treatment with cisplatin ±APX3330 treatment. Cisplatin and APX3330 were administered concurrently for 3 weeks (Day 0-Day 17) and endpoints of neuronal toxicity were assessed within the DRG of mice at several time points following the last dose of cisplatin.

[0084]When isolated sensory neurons were exposed to APX3330, a concentration-dependent increase in Ref-1 / APE1 endonuclease activity occurred, which is not observed in tumor cells. Although APX3330 is a targeted inhibitor of Ref-1 / APE1's redox function, it appears that, in the setting of sensory neurons, it can also enhance the protein's DNA repair (AP endonuclease) activity (FIGS. 7A-7E). APX3330 causes the protein to unfold over time. This unfolding primarily a...

example 3

[0086]In this Example, APX3330 analogs were analoyzed for their ability to protect against neurotoxicity-induced by cisplatin or oxaliplatin while not diminishing the anti-tumor effect of the platinum. Also, the analog APX2009 was assessted for its anti-tumor effects in neuroblastoma cell lines as well as in a 3D spheroid pancreatic tumor model.

[0087]Materials and Methods

[0088]Materials

[0089]General tissue culture supplies were obtained from Invitrogen (Carlsbad, Calif.), and chemicals were purchased from Sigma-Aldrich (St. Louis, Mo.). For sensory neuronal cultures, poly-D-lysine and laminin were purchased from Sigma-Aldrich (St. Louis, Mo.), nerve growth factor from Harlan Bioproducts for Science (Indianapolis, Ind.), and normocin from Invivogen (San Diego, Calif.). Mouse monoclonal antihuman APE1 antibodies were raised in the laboratory and are available from Novus Biologicals (Littleton, Colo.). Mouse monoclonal anti-phospho-H2AX antibodies were from EMD Millipore (Billerica, Ma...

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Abstract

Methods of reducing neuronal sensitivity, thereby reducing inflammation and chronic pain, are disclosed herein. Particularly disclosed are methods of administrating the apurinic/apyrimidinic endonuclease 1 redox factor 1 (APE1/Ref-1) inhibitor, APX3330, to enhance the DNA base excision repair (BER) pathway, thereby reducing neuronal sensitivity to inflammatory mediators and alleviating inflammatory or chronic pain.

Description

CROSS-REFERENCE OT RELATED APPLICATION[0001]This application claims the benefit to U.S. Provisional Patent Application No. 62 / 486,033, filed on Apr. 17, 2017, which is hereby incorporated by reference in its entirety.STATEMENT OF GOVERNMENT SUPPORT[0002]This invention was made with government support under NS091667 awarded by the National Institutes of Health (NIH). The government has certain rights in the invention.INCORPORATION OF SEQUENCE LISTING[0003]A computer readable form of the Sequence Listing containing the file named “IURTC_2017-116-02_ST25.txt”, which is 1,070 bytes in size (as measured in MICROSOFT WINDOWS® EXPLORER), is provided herein and is herein incorporated by reference. This Sequence Listing consists of SEQ ID NOs: 1-4.BACKGROUND OF THE DISCLOSURE[0004]The present disclosure relates generally to methods of reducing neuronal sensitivity, thereby reducing inflammation and chronic pain. Particularly, it has been found herein that by enhancing the DNA base excision r...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K31/192A61P25/02A61N5/00A61K33/243
CPCA61K31/192A61P25/02A61N5/00A61K45/06A61K31/282A61K31/165A61K31/201A61K31/555A61K31/122A61K33/243A61K2300/00A61K33/24
Inventor KELLEY, MARK R.FEHRENBACHER, JILL
Owner INDIANA UNIV RES & TECH CORP
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