Dairy starter culture delivery system and method thereof
a technology of starter culture and delivery system, which is applied in the directions of milk preparation, buttermilk, food preparation, etc., can solve the problems of contaminating the process line, restricting the growth of contaminating microorganisms, and affecting the quality of the produ
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example 1
Stability of starter cultures in aqueous suspensions containing a milk clotting enzyme
The objective of the experiment was to test the stability of commercial mesophilic and thermophilic lactic acid bacterial starter cultures in an aqueous suspension containing a milk clotting enzyme.
1.1 Materials and methods
Test cultures
(i) Frozen DVS-culture, LD-Culture CH-N 11.TM. (Chr. Hansen A / S, H.o slashed.rsholm, Denmark);
(ii) Frozen DVS-culture, O-Culture R-604.TM. (Chr. Hansen A / S, H.o slashed.rsholm, Denmark;
(iii) Frozen DVS-culture, S. thermophilus TH-4.TM. (Chr. Hansen, H.o slashed.rsholm, Denmark.
The CH-N 11 culture is a multiple mixed strain culture containing Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp. lactis, Leuconostoc mesenteroides subsp. cremoris and Lactococcus lactis subsp. diacetylactis. The culture produces aroma and CO.sub.2. The cell concentration is at least 1.times.10.sup.10 cfu / g.
The culture has an indicated activity in terms of acidification of an aque...
example 2
Stability of starter cultures in aqueous suspensions
The purpose of the experiment was to test the stability of commercial and experimental lactic acid bacterial starter cultures after suspension in an aqueous medium under different storage conditions.
2.1 Materials and methods
A range of commercial and experimental cultures for making Cheddar cheese was tested. The cultures tested included:
Experimental protocol:
The starter cultures were tested for acidification activity at an inoculation rate of 0,002% wt / vol for freeze dried cultures and 0,01% wt / vol for frozen cultures with respect to the culture in an aqueous suspension.
Step 1: In the first step the culture was suspended in an aqueous medium and kept at 10.degree. C. for 24 hours, where nothing else is stated.
Step 2: The activity of the culture suspension obtained in step 1 was tested in pasteurised whole milk using the standard Pearce test (IDF No. 129, 1980).
pH of the suspension in step 1 and the inoculated milk of step 2 was mon...
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