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Nanopore array structured devices for biosensing and energy storage

a structured device and nanopore technology, applied in the field of biosensors, can solve the problems of insufficient robustness, limited development of glucose sensor technology, and insufficient development stage of technology, so as to avoid biofouling and denaturation, and improve the efficiency of the system

Active Publication Date: 2014-02-04
CHEN ELLEN T
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conventional glucose sensor technologies have limited the development of glucose sensors, especially in its measurements of blood glucose in the hypoglycemia range (see reference 23).
Direct measurement of analyte without using mediators is one the advantages that this type of sensor offers because the mediators are leachable from the polymer network and are toxic.
However, as the reviewers Vaseashta and Irudayaraj point out, the technology is still in the development stage and the robustness has not been established (see reference 13).
As indicated above, however, robust nanopore structured sensors have yet to be reported.
Especially for most implantable medical devices, they need battery power up, however, higher power consumption means shorter lifetime of the device.
For a remotely powered device, higher power consumption will decrease its communication distance.
Moreover, more heat will be generated due to higher power consumption from the implant which may damage the contact tissue.
Implantable biosensors are facing design challenges of signal drafting, corrosion; frequent calibration and instability due to body fluid interference (reference 1-5).
For example, the implantable glucose biosensors used for continuous monitoring glucose for type 1 diabetes and for monitoring glucose in trauma brain injured (TBI) tissue, are prone to above mentioned drawbacks.
The conventional methods for detection of low abundant cancer biomarkers are both time consuming and costly.
The current methodologies are impractical with respect to a global health setting.
Current imaging technologies are not capable of quantitative detection of the early stage cancers.
This technique depends on biopsy samples, which is invasive and inaccurate; the enzymatic reactions are subject to interference.
The more traditional antibody immunosensor technology is both time consuming and costly.

Method used

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Examples

Experimental program
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Effect test

example 1

Constructing the Biosensor

[0064]Reagent grade poly (4-vinylpyridine) (PVP), polyethylene glycol diglycidyl ether (PEG), triacetyl-β-CD (T-β-CD), β-CD / epichlorohydrin, β-D-glucose were purchased from Aldrich-Sigma. The PVP was recrystallized in methanol. The biomimetic glucose enzyme, which is a biomimetic Histidine residue (His-516) receptor of glucose oxidase and mimics the active center of native glucose enzyme, named mM-β-DMCD was synthesized generally according to the published procedures (E. T. Chen and H. L. Pardue, Analytical applications of catalytic properties of modified-cyclodextrins. Anal. Chem. 65, 2563-2567, 1993, which is hereby incorporated by reference in its entirety as if set forth herein). U.S. Pat. No. 6,582,583 issued on Jun. 24, 2003 is also hereby incorporated by reference in its entirety as if set forth herein. Briefly, β-DMCD may be reacted first with sodium hydride in dry tetrahydrofuran under a nitrogen atmosphere at 35-38° C. for 10 hours. The solution i...

example 10

Glassy Carbon Electrode

[0089]In addition to gold, glassy carbon can be used for construction of the biosensor of the present invention. The DET effect was observed and the irreversible peaks were also obtained.

[0090]The foregoing is considered as illustrative only of the principles of the invention. Further, since numerous modifications and changes will readily occur to those skilled in the art, it is not desired to limit the invention to the exact construction and operation shown and described, and, accordingly, all suitable modifications and equivalents may be resorted to, falling within the scope of the invention.

[Following Examples are for CIP Application]

example 11

Constructing the Biosensor

[0091]Reagent grade poly(4-vinylpyridine) (PVP), polyethylene glycol diglycidyl ether (PEG), triacetyl-β-CD (T-β-CD) and β-CD / epichlorohydrin were purchased from Aldrich-Sigma. PVP was purified before use. The biomimetic glucose enzyme, which is a biomimetic Histidine residue (His-516) receptor of glucose oxidase and mimics the active center of native glucose enzyme, named mM-β-DMCD was synthesized generally according to the published procedures (E. T. Chen and H. L. Pardue, Analytical applications of catalytic properties of modified-cyclodextrins. Anal. Chem. 65, 2563-2567, 1993, which is hereby incorporated by reference in its entirety as if set forth herein). U.S. Pat. No. 6,582,583 issued on Jun. 24, 2003 is also hereby incorporated by reference in its entirety as if set forth herein. Briefly, β-DMCD may be reacted first with sodium hydride in dry tetrahydrofuran under a nitrogen atmosphere at 35-38° C. for 10 hours. The solution is then cooled to 0° C....

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PUM

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Abstract

The present invention provides a novel device for biosensing and energy storage. The present invented device comprises an electrode having a nanopore structured and bio-communicationally active cyclodextrin attached thereto. The device has demonstrated robust analytical performances for direct single subtype breast cancer measurements without mediators, or native enzyme, and without antibodies labeling; the device is capable to store energy by direct bio-communication with the living cancer cells at real time is demonstrated. It is beneficial in the health care diagnostic applications.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This patent application claims the benefit of U.S. patent application Ser. No. 11 / 785,660 filed on Apr. 19, 2007, is a continuation in part application, that claims benefit of U.S. Provisional Patent Application Ser. No. 60 / 792,902 filed Apr. 19, 2006. The entire disclosure of the prior Patent Application Ser. No. 60 / 792,902 is hereby incorporated by reference, as is set forth herein in its entirety.STATEMENT REGARDING FEDERAL RESEARCH[0002]This invention was made with government support through the U.S. Department of health and Human Services, U.S. Food and Drug Administration. The United States Government has certain rights in this invention.FIELD OF THE INVENTION[0003]The present invention relates to the field of biosensors and, in particular, to biosensors comprising a catalytically active cyclodextrin in a nanopore form.BACKGROUND OF THE INVENTION[0004]Monitoring blood glucose levels regularly is very important in proper diabetes man...

Claims

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Application Information

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Patent Type & Authority Patents(United States)
IPC IPC(8): G01N27/327
CPCG01N2610/00G01N33/548G01N33/57492
Inventor CHEN, ELLEN, TUANYING
Owner CHEN ELLEN T
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