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Biological induction type active artificial cornea, and application method therefor

An artificial cornea and inducible technology, applied in eye implants, medical science, prostheses, etc., can solve the difficulties in the way and method of obtaining and cultivating corneal cells, difficulty in immune rejection of allogeneic corneal cells, prolapse of artificial cornea, etc. problems, to facilitate large-scale production and industrialization, to facilitate long-term storage and long-distance transportation, and to avoid immune rejection

Inactive Publication Date: 2007-10-03
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are two kinds of artificial corneas: inactive artificial cornea and non-biologically induced tissue engineering artificial cornea. Inactive artificial cornea is made of artificial cornea made of synthetic materials such as methyl methacrylate (MMA), and is being transplanted into human body. Although it can restore people's vision, but the field of vision is extremely small, and it is difficult for artificial synthetic materials to fuse with the tissues around the eye. Complications such as internal inflammation bring great pain to patients
The general concept of tissue engineering artificial cornea is to culture corneal cells under the condition of strengthening in vitro, and the cells cultured in vitro grow on the scaffold material before making it biologically active; although the artificial cornea produced by this method is in the The compatibility with surrounding tissues of the eye may be improved, but because it needs to culture a variety of keratocytes in vitro, and the current ways and methods of obtaining and culturing keratocytes are relatively difficult and immature, the preservation and maintenance of keratocytes after culture There are also problems in subculture. The whole process is complicated and expensive. The immune rejection of allogeneic keratocytes in the transplantation process is also a major problem.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The ratio of each component of the biologically induced active artificial cornea is as follows:

[0025] Chitosan 2%

[0026] Collagen 2.5%

[0027] Hyaluronic Acid 0.3%

[0028] Water 95.1%

[0029] The above ratios are percentages by weight.

[0030] The process of preparing the bioinducible active artificial cornea with the above-mentioned components includes the following steps: (1) weighing each component according to the above-mentioned dosage, dissolving chitosan in hydrochloric acid solution, dissolving collagen and hyaluronic acid in water; (2) The aqueous solution of chitosan hydrochloric acid and collagen and hyaluronic acid was stirred at room temperature for 1.5 hours, so that each component was fully mixed; (3) 0.1% carbodiimide was added to the mixed solution Cross-linking agent, 90ng / ml fiber-forming growth factor, 35ng / ml epidermal growth factor and 60ng / ml cell transforming growth factor, mixed thoroughly for half an hour; (4) inject the mixed solu...

Embodiment 2

[0033] The ratio of each component of the biologically induced active artificial cornea is as follows:

[0034]Chitosan 1%

[0035] Collagen 3%

[0036] Hyaluronic Acid 0.2%

[0037] Water 95.65%

[0038] The above ratios are percentages by weight.

[0039] The process of preparing the bio-inducible active artificial cornea with the above-mentioned components includes the following steps: (1) weighing each component according to the above-mentioned dosage, dissolving chitosan in acetic acid solution, dissolving collagen and hyaluronic acid in water; (2) The aqueous solution of chitosan acetic acid solution and collagen and hyaluronic acid was stirred at room temperature for 2 hours, so that each component was fully mixed; (3) 0.15% carbodiimide was added to the mixed solution Cross-linking agent, 5ng / ml fiber-forming growth factor, 20ng / ml epidermal growth factor and 10ng / ml cell transforming growth factor, mixed thoroughly for half an hour; (4) inject the mixed solution i...

Embodiment 3

[0042] The ratio of each component of the biologically induced active artificial cornea is as follows:

[0043] Chitosan 0.5%

[0044] Collagen 1.5%

[0045] Hyaluronic Acid 0.3%

[0046] Water 97.7%

[0047] The above ratios are percentages by weight.

[0048] The process of preparing the bio-inducible active artificial cornea with the above-mentioned components includes the following steps: (1) weighing each component according to the above-mentioned dosage, dissolving chitosan in acetic acid solution, dissolving collagen and hyaluronic acid in water; (2) Stir the aqueous solution of chitosan acetic acid solution and collagen and hyaluronic acid at room temperature for 2 hours to fully mix the components; (3) inject the mixed solution into the prefabricated corneal mold and fill the corneal mold Put it in an oven for drying and take it out; (4) Soak and dry the corneal product obtained by soaking in clean water to remove residual impurities, and place it in water for 24 ...

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Abstract

A biological induction type active artificial cornea is prepared from chitosan, collagen, hyaluronic acid, water, and optional growth factor. It can be directed transplanted in human eye. Its advantages are high biological compatibility, easy preparing and long storage time.

Description

(1) Technical field [0001] The invention relates to artificial cornea technology, in particular to a biologically induced active artificial cornea and its application method. (2) Background technology [0002] Corneal disease is currently the main cause of eye blindness. There are about 10 million patients worldwide, and there are about 3 million corneal blind patients in my country. However, due to the lack of donor sources, only about 1,000 cases of allogeneic keratoplasty can be performed in my country every year, and a large number of patients cannot be treated due to the shortage of human corneas. This is a global problem that has not been solved for a long time. Due to the ocular immune pardon phenomenon and the avascular nature of the cornea, keratoplasty is the operation with the highest success rate in whole body tissue and organ transplantation. Therefore, artificial cornea and its transplantation are superior to other tissue engineering organs and tissue transplan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/50A61F2/14
Inventor 黄耀熊李沁华
Owner JINAN UNIVERSITY