Immunoassay detection method for neonicotinyl insecticides
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An immunoassay and pesticide technology, applied in the field of immunoassay to achieve the effect of easy operation
Inactive Publication Date: 2008-04-09
SYNGENTA PARTICIPATIONS AG
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However, many serious limitations of classical analytical methods remain
Method used
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example 1
[0062] Example 1: Hapten (IIIA)
[0063]
[0064] where A, R 3 , X, and n have the same definitions as in general formula IA.
[0065] Hapten (IIIB)
[0066]
[0067] where A, R 3 , and X are defined the same as in general formula IA, and E is defined in the same way as in general formula IB.
[0068] Haptens of general formula (IIIA) and (IIIB) can be prepared methodologically analogously as follows:
[0069]
[0070] The desired haptens (IIIA) or (IIIB) are prepared using appropriate 2-aminoethanethiol derivatives selected from compounds having the following general formula:
[0071]
[0072] wherein R has the meanings given above for general formula (I).
[0073] The following haptens have been found to be particularly useful in the practice of the invention:
[0074]
[0075] Where n in each case is an integer of 1-5, preferably an integer of 3-5.
[0076] As the case may be, antigenic neonicotinoid conjugates (immunogens) can be generated and assayed...
Embodiment 1
[0093] Example 1: Synthetic process of thiamethoxam hapten
[0094]
[0095] 1.12-Methyl-1-nitroisourea
[0096] O-methylisourea sulfate (38.5 g) was dissolved in nitric acid (120 ml) / sulfuric acid (280 ml) mixture at 0°C, and stirred at 0°C for 2 hours. Carefully pour the mixture onto crushed ice and stir, then filter to remove the crushed ice. The needle-like crystals were dissolved in ethyl acetate, and potassium carbonate was added to make the solution alkaline. The solution was dried over magnesium sulfate and filtered. The filtrate was concentrated to give a white powder (5g).
[0097] 1.2
[0098] A solution was prepared from 3 g of methyl 4-aminobutyrate hydrochloride (1.1a) and 15 ml of water. The pH was adjusted to 11 by the dropwise addition of 2N NaOH while cooling the solution in an ice bath. The reaction flask was equipped with a pH electrode and a thermometer, and the resulting clear, alkaline solution was stirred rapidly while 2-methyl-1-nitroisourea (...
Embodiment 2-5
[0106] Examples 2-5: thiamethoxam haptens
[0107] The synthetic methodology of Example 1 was used to prepare the following thiamethoxam haptens shown in Table 1, wherein the haptens of the general formula NH 2 (CH 2 ) n CO 2 R(1.1b) (where n is an integer from 1 to 5, R is H or C 1 -C 4 Alkyl) appropriate analogues were carried out instead of intermediate 1.1a.
[0108] Table 1
[0109]
[0110] Example
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Abstract
The present invention provides immunogens for generating anti-neonicotinoid antibodies as well as antibodies, methods, reagents, and kits for determining the presence of one or more neonicotinoid insecticides in a sample by immunoassay. The present invention has particular application to determination of the concentration of neonicotinoid insecticides applied to a plant propagation materials such as seed, said antibody is selective for a compound of formula (I) wherein A is 2-chloropyrid-5-yl or 2-chlorothiazol-5-yl; R and R<3> independently are hydrogen or C1-C4alkyl; R<1> and R<2> independently are hydrogen or C1-C4alkyl or R<1> and R<2> are taken together with the nitrogen atoms to which they are attached to form an imidazoline or an oxadiazine ring; and X is N-NO2 or N-CN. Also claimed is a compound of formula (IA) and protein conjugate of formula (II) in which PR is a protein residue of a carrier molecule selected from the group consisting of a purified protein derivative (PPD, Tuberculin) from Diptheria virus, bovine serum albumin, cationized bovine serum albumin, human serum albumin, ovalbumin and keyhole limpet hemocyanin; or an enzyme residue selected from the group consisting of alkaline phosphatase, horseradish peroxidase, and beta -galactosidase.
Description
field of invention [0001] The present invention relates to immunoassays for pesticides, and antibodies and reagents for performing such assays. The invention also relates to methods of performing such assays, as well as kits comprising reagents for practicing such methods. The invention is specifically applied to the detection and quantification of neonicotinoid insecticides in samples. Background of the invention [0002] insecticide [0003] Synthetic insecticides are commonly used to control insect pests in agricultural crops. This practice has achieved a high degree of commercial success, as this control can significantly increase crop yields. However, effective use of insecticides requires sound management, taking into account issues of insect resistance and environmental and worker exposure. One solution to this problem is to provide new, more highly active insecticides, thereby reducing the need for the acutely toxic insecticides of the past and reducing the rate ...
Claims
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Application Information
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