Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation process of chito oligosaccharace hydrochloride

A technology of hydrochloride and chitosan oligosaccharides, which is applied in food preparation, medical preparations containing active ingredients, oligosaccharides, etc., can solve problems such as short shelf life, loss of water solubility and activity, and easy brownish black. Achieve the effect of low cost and high yield

Inactive Publication Date: 2008-05-07
孝感学院
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these products have a short shelf life, tend to turn brownish black in the air and at higher temperatures, and lose water solubility and activity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: Get 5 grams of lobster shell chitosan (deacetylation degree 88%), dissolve with 200 milliliters of 1% dilute hydrochloric acid, filter, add sodium hydroxide to filtrate and make chitosan separate out, wash with water until eluent pH is 9. Dissolve in 75ml of 0.6wt% hydrochloric acid, add 0.1g of α-amylase, react in a water bath at 50°C for 1 hour, add hydrochloric acid to adjust the pH of the solution to 5, filter through a 10kDa vacuum fiber membrane, and then concentrate using a nanofiltration membrane , the filtrate was added with concentrated hydrochloric acid to adjust the pH of the solution to 2, and freeze-dried to obtain the chitosan oligosaccharide hydrochloride product.

Embodiment 2

[0014] Embodiment 2: Get commercially available crab shell chitosan (deacetylation degree 86%) 110 grams, soak with 100 milliliters of water, add 2700 milliliters of 0.4wt% hydrochloric acid, stir 4 hours, add R-10 cellulase 3 grams, in React at 40°C, after half an hour, add hydrochloric acid to adjust the pH of the solution to 5.5, when the polymerization degree of the main component of the chitosan product drops below 30, filter, add hydrochloric acid, adjust the pH of the solution to 2, boil for about 10 minutes, filter to remove After the enzyme, concentrate under reduced pressure at 50°C, add ethanol to settle and wash, centrifuge, and dry to obtain the chitosan oligosaccharide hydrochloride product.

Embodiment 3

[0015] Embodiment 3: Get 80 grams of commercially available shrimp chitosan (deacetylation degree 93%), soak with 100 milliliters of water, add 1100 milliliters of 0.9wt% hydrochloric acid, stir for 4 hours, add 8 milliliters of enzyme liquids (produced in Wuhan, containing Hemicellulase and chitosanase), react at 48 ℃, after one hour, adjust the solution pH=5, when the main component polymerization degree of chitosan product drops to about 30, filter, use NMWL 10kDa, 3kDa and The filtrates of the three components were obtained by fractionation with a 1kDa ultrafiltration membrane, and the pH of the solution was respectively adjusted below 3. After vacuum concentration, freeze-drying, the chitosan oligosaccharide hydrochloride product was obtained.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A process for preparing oligochitosan hydrochloride used to prepare medicine, health-care food, etc for regulating immunity and preventing and treating tumor and arthritis includes such steps as adding chitosan into reactor, proportionally adding the solution of hydrochloric acid, stirring while controlling the concentration of chitosan to 2-10 Wt%, adding enzyme reagent, reacting while stirring, filtering and purifying.

Description

technical field [0001] The invention relates to a preparation method of chitosan oligosaccharide hydrochloride, the prepared product is easy to preserve, and the product can be used as a raw material for anti-tumor, immunity-regulating and arthritis medicines and health care products, or as cosmetics and food additives. Background technique [0002] As an alkaline polysaccharide from animals and plants, chitin is non-toxic, harmless, rich in sources, and has many unique physiological activities. It is considered as the "sixth element of life". Oligosaccharides in mammalian milk contain acetylglucosamine units. However, with the improvement of living conditions, people's chances of ingesting chitin from coarse food are reduced, so necessary supplements are needed. However, chitin is insoluble in water and acid. Chitosan is a derivative of chitin after deacetylation. It is soluble in acid solution, but insoluble in neutral medium, which affects its digestion and absorption i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08C07H3/06A61K31/722A61P19/02A23L1/30A23L33/125
Inventor 覃彩芹曾林涛宋学建
Owner 孝感学院