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Gluconobacter and its use

A technology of gluconoacetic acid bacteria and acetic acid bacteria, applied in gluconoacetic acid bacteria and its application fields, can solve the problems of unclear specific strains of D-glucaric acid 1,4 lactone and lack of large-scale industrial production , to achieve broad industrial applications and market prospects, low cost, and simple fermentation process

Inactive Publication Date: 2008-06-04
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Kombucha contains D-glucaric acid 1,4 lactone, but, because kombucha contains membrane acetobacter, a layer of bacterial cellulose film will be formed on the surface of the liquid during fermentation production, so the production of kombucha is currently carried out on a small family scale Liquid static culture is the main method, and large-scale industrial production has not yet been formed, and the specific strain that produces D-glucaric acid 1,4 lactone is still unclear

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0028] Example 1. Screening of Gluconacetobacter sp. CGMCC № 1157 Acetobacter solid medium: glucose 100g, yeast extract 10g, calcium carbonate 20g, agar 15g, distilled water 1000mL, pH 6.8, extinguished at 121°C bacteria for 15 minutes.

[0029] Sugar tea water culture medium: 5g / 100ml glucose, the final concentration of solids is 0.5 Baumé tea extract, pH 4.9.

[0030] The screening steps of Gluconacetobacter sp. Jlab A4 (Gluconacetobacter sp.) are as follows:

[0031] 1. Preliminary screening

[0032] 1. After oscillating and mixing the activated kombucha mixed strains (sampled in folks in Beijing), take 10mL of bacterial liquid and a small piece of bacterial film into 90mL of sterile water with glass beads, fully oscillate and mix; absorb 5mL to 45mL sterile water, mix well and then dilute to 10 -2 、10 -3 、10 -4 、10 -5 and 10 -6 , draw 10 respectively -4 、10 -5 and 10 -6 Spread 0.1mL of the three dilutions of the bacterial solution on the solid medium of Acetobact...

Embodiment 2

[0053] Embodiment 2, the fermentation production of gluconacetobacter sp. (Gluconacetobacter sp.) Jlab A4 CGMCC № 1157

[0054] Include the following steps:

[0055] 1. Cultivation of Gluconacetobacter sp. Jlab A4 CGMCC № 1157 seed solution

[0056] Acetobacter liquid medium: glucose 100g, yeast extract 10g, calcium carbonate 20g, distilled water 1000ml, pH 6.8, sterilized at 121°C for 15min.

[0057] Use an inoculation loop to pick 1 loop of the strain from the slant and inoculate it in 10 mL of Acetobacter liquid medium, culture it with shaking at 30°C for 24 hours, and then inoculate it in sugar tea water medium at a ratio of 20% (volume ratio) for continuous Two expansion cultures were carried out at 30°C for 24 hours with shaking.

[0058] 2. Fermentation

[0059] Sugar tea water medium: Yunnan Dianhong tea leaf extract with a solid concentration of 0.5 Baume, 5g / 100ml glucose, pH 4.9.

[0060] Inoculate the seed solution of step 1 in the ratio of 10% (volume ratio) t...

Embodiment 3

[0061] Embodiment 3, the fermentation production of gluconacetobacter sp. (Gluconacetobacter sp.) Jlab A4 CGMCC № 1157

[0062] Include the following steps:

[0063] 1. Cultivation of Gluconacetobacter sp. Jlab A4 CGMCC № 1157 seed solution

[0064] With embodiment 2.

[0065] 2. Fermentation

[0066] Inoculate the seed liquid of step 1 in the ratio of 10% (volume ratio) to sugar tea water medium (except pH 4.0, others are the same as in Example 2) (150mL medium in a 250mL Erlenmeyer flask), and culture at 25°C and 160rpm with shaking After 6 days, the content of D-glucaric acid 1,4-lactone in the fermentation broth was determined to be 3.46 mg / mL after the fermentation.

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Abstract

This invention relates to application of Gluconacetobacter sp.applying a new strain to produce D- saccharonolactone (1,4). The strain is Gluconacetobacter sp.Jlab A4 CGMCC No.1157.Control pollution and prepare surface active agent. Culture the strain on the sugar tea culture medium to obtain the D- saccharonolactone (1,4). The strain is efficient 3-5mg / ml. The process is facility and economical.

Description

technical field [0001] The invention relates to a strain of gluconoacetic acid bacteria and its application, in particular to a strain of gluconoacetic acid bacteria and a method for producing D-glucaric acid 1,4 lactone by using the strain. Background technique [0002] Kombucha (kombucha), also known as black tea mushroom, tea mold, Haibao, is a kind of folk traditional acidic drink made from sugary tea extract through microbial fermentation. Kombucha is a fermented product of multiple strains, mainly yeast, acetic acid bacteria and lactic acid bacteria. After research, kombucha has the health care function of preventing and curing diseases and prolonging life. For example, it has the functions of enhancing the body's immunity, longevity, reducing wrinkles, preventing cancer, anti-cancer, losing weight, lubricating the intestines, enhancing the functions of the heart, brain, liver and kidney, and reducing the risk of obesity. Blood pressure, sleeping, helping digestion, c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/62C12P19/00C12R1/01
Inventor 籍保平田文礼李博吴薇杨志伟盖宝川张泓周峰
Owner CHINA AGRI UNIV
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