Buckthorn extract, its preparing method and use in pharmaceutical process

A technology of extract and seabuckthorn, which is applied in the application field of preparation of medicines, to achieve the effect of increasing radiosensitivity, simple and practical method, and clear structure

Inactive Publication Date: 2009-01-07
水利部沙棘开发管理中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no report of extracting anti-inflammatory active substances from natural Chinese herbal medicine plants

Method used

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  • Buckthorn extract, its preparing method and use in pharmaceutical process
  • Buckthorn extract, its preparing method and use in pharmaceutical process
  • Buckthorn extract, its preparing method and use in pharmaceutical process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Preparation of extract and determination of compound structure

[0028] 3.0 kg of Hippophae rhamnoides bark was crushed, 10 liters of 80% acetone (acetone: water = 8: 2) was added, and left at room temperature for 12 hours. Filter to obtain the extract. The extracted seabuckthorn powder was extracted again with 10 L of 80% acetone, and mixed with the previously obtained extract. This solution was concentrated under reduced pressure at 40°C to obtain 929.8 g of a brown powder extract.

[0029] Take 929.8g of powder extract and dissolve it in 3L of water, and then use 2L each of n-hexane, chloroform, ethyl acetate and butanol to extract in sequence. The extract was concentrated under reduced pressure at 40°C. The n-hexane layer obtained 21.14g of extract, the chloroform layer obtained 29.34g of extract, the ethyl acetate layer obtained 37.12g of extract, and the n-butanol layer obtained 180.23g of extract. The aqueous layer yielded 631.73 extracts. The NO p...

Embodiment 2

[0035] Embodiment 2: Determine the structure of physical and chemical parameters and compounds

[0036] The character, molecular weight etc. of compound (4) are shown in Table 1; Compound (4) 13 C-NMR, 1 The H-NMR data are shown in Table 2. And finally determined the structural formula of the relevant compound by the above data.

[0037] Table 1: Properties, molecular weight, UV, IR of compound (4)

[0038]

[0039] The NMR spectral data (ppm, in Methanol-d) of table 2 compound 4 4 600MHz)

[0040] s: singlet d: doublet t: triplet m: multiplet

[0041] () value is Hz

[0042]

[0043] The structural formula of compound 4 is determined as follows:

[0044]

Embodiment 3

[0045] Embodiment 3: Inhibition of NO production test

[0046] The inhibitory effect on NO production (NO production inhibitory effect) of megalithic phagocytes stimulated by interferon-r and lipopolysaccharide was obtained by the following experimental method. And the inhibitory effect of NO production was evaluated based on the IC50(um) of the inhibitory effect.

[0047] Materials used:

[0048] RAW 264.7 cells (Dainippon Pharmaceutical Co., Ltd.)

[0049] N-1-naphthaleneethylenediamine hydrochloride (1g Wako Pure Chemical Industries)

[0050] Sulfa (500g Wako Pure Chemical Industries)

[0051] Ham's F12 Medium (SIGMA N488 500mL)

[0052] IFN-γ (Geneyme / Techne 100μg)

[0053] Lipopolysaccharide (LPS, 055:B5 10mg, Sigma)

[0054] Phosphoric acid (500ml Wako Pure Chemical Industries)

[0055] DMSO (500ml Wako Pure Chemical Industries)

[0056] 96-well microtiter plate (50 / box Sumitomo Bakelite, trade name [8096R])

[0057] experiment method:

[0058] RAW264.7 cells, ...

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Abstract

The compound extracted from sallow thron berry includes four kinds, in which three kinds are active compounds. The sallow thron berry extract and its salt, enatiomorph, racemic body, tautomer or physiological function derivative can be used for preparing medicine for curing or preventing inflammatory diseases. It also can be used for preparing health-care food, beverage or feed.

Description

technical field [0001] The invention relates to seabuckthorn extract, in particular to three active compounds extracted from seabuckthorn, its preparation method and its application in preparing medicine. Background technique [0002] Daily inflammatory diseases that plague people include: joint inflammation, such as osteoarthritis, rheumatoid arthritis, rheumatoid spondylitis, gouty arthritis, etc.; inflammatory skin diseases, such as eczema, psoriasis, dermatitis, etc.; inflammatory eye diseases , such as uveitis, conjunctivitis, etc.; lung diseases, such as asthma, bronchitis, acute respiratory distress syndrome, etc.; bacteremia, toxemia, aphthous ulcers, gingivitis, pancreatitis, etc.; gastrointestinal tract Diseases such as Crohn's disease, atrophic gastritis, ulcerative colitis, celiac disease, peptic ulcer, irritable bowel syndrome, e.g. Gastrointestinal disease caused by anti-inflammatory drugs and so on. [0003] The mechanism of various inflammatory pathogenic e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/56A61P29/00A61P19/02A61P1/00A61P11/00A61P9/00A61P25/00A61P3/10A61P13/12A61P17/00A61P27/02A61P37/06A23L1/30
Inventor 邰源临卢顺光杨志刚李永海温中平北中进大根谷章浩王立岩赵十一都留信也
Owner 水利部沙棘开发管理中心
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