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Specific bacteria preparation and method for treating chemical waste water or conventional organic waste water

A technology with special effects and inoculants, applied in chemical instruments and methods, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems of high cost and selling price, achieve low production costs, improve treatment efficiency, and high flocculation. Effect

Inactive Publication Date: 2009-06-10
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high cost and selling price, domestic manufacturers generally do not use imported special bacteria agents for wastewater treatment, and domestic products in this area are still blank.

Method used

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  • Specific bacteria preparation and method for treating chemical waste water or conventional organic waste water
  • Specific bacteria preparation and method for treating chemical waste water or conventional organic waste water
  • Specific bacteria preparation and method for treating chemical waste water or conventional organic waste water

Examples

Experimental program
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Embodiment Construction

[0030] The fermentation process of the preparation technology of NJU-Fhhh1 special-effect bacterial agent

[0031] Example name: Preparation of NJU-Fhhh1 special effect bacterial agent and its degradation of purified terephthalic acid PTA petrochemical wastewater (1) Application of NJU-Fhhh1 special effect bacterial strain to prepare NJU-Fhhh1 special effect bacterial agent condition control Preparation of bacterial agent

[0032] Bioreactor: B.Braun fully automatic bioreactor made in Germany, with an effective volume of 1L;

[0033] Automatic control parameter setting: DO (dissolved oxygen) = 2mg / L; pH = 7.0; T = 35 ° C; Sf (liquid medium flow rate) = 0.33 (V / V);

[0034] Stirring speed: 1000rpm; foam removal: silicone oil; liquid level: up to 1L effective volume; mineral salt flow rate: 0.003 (V / V); liquid medium: same as the above liquid medium

[0035] (2) Determination of parameters of NJU-Fhhh1 special bacterial agent preparation

[0036] NJU-Fhhh1 special bacteria bio...

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Abstract

The invention is a method to prepare the specific bacterium agent which fuses the protoplast of white-rotten ephiphyte, aboriginal bacterium YZ1 and vintage yeast, and by the gene's reformation and conformity, structures the gene engineering bacterium. The ferment parameters: ferment temperature 33 plus and minus 2 C degree, pH value 7.0 plus and minus 1; soluble oxygen in reactor >=2mg / L; mineral salt flux 0.001-0.005V / (V.d); thalli concentration 2-10 g / L; cultivating it in the culture fluid containing C, P and N. The maximal degradation area of NJU-Fhhh1 quick-degradation extract to phenyl-dimethyl acid petrochemical waste water: qmax=0.226 / h.

Description

1. Technical field [0001] The invention relates to a biotechnology for preparing special-effect microbial agent and its application. 2. Background technology: [0002] There are many kinds of special-effect bacterial agents for treating wastewater in the world, but there is no patent on the preparation and application of protoplast fusion bacterial agents to treat wastewater. A typical example is EM in Japan, and HIMP (High-Intelligence Microorganism Preparation) from PARADISF in the United States, 1 liter / 24 yuan. Due to the high cost and selling price, domestic manufacturers generally do not use imported special-effect bacteria agents for wastewater treatment, and domestic products in this area are still blank. 3. Contents of the invention [0003] The purpose of the present invention is to provide a method for preparing and applying NJU-Fhhh1 special-effect bacterial agent to efficiently treat wastewater. In particular, the original genetically engineered special-effe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/02C12N15/08C12N1/00C02F3/32
Inventor 程树培严俊石磊张徐祥郝春博阙子龙陈俊顾继东李维新黄秋明于红霞
Owner NANJING UNIV