Method for preparing gamma-aminobutyric acid by using rice grain as raw material
A technology of aminobutyric acid and rice, which is applied in the field of preparation of γ-aminobutyric acid, can solve the problems of low production cost, low production rate and high production cost, and achieves the advantages of less environmental pollution, high product activity and low production cost. Effect
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Embodiment 1
[0011] (1) Weigh 4 kg of rice, crush it into a fineness of 90 mesh, add 24 kg of drinking tap water, soak for 7 hours, heat to 75 ° C for 15 minutes to gelatinize; (2) add 20 grams of starch to the gelatinized rice paste Enzyme, incubate at 40°C for 65 minutes to prepare amylase-treated rice milk; add 12 grams of protease to the amylase-treated rice milk and incubate at 35°C for 45 minutes to prepare protease-treated rice milk; heat protease Keep the treated rice milk at 90°C for 5 minutes to prepare rice milk; (3) after the temperature of the rice milk drops to room temperature, inoculate the expanded cultured γ-aminobutyric acid-producing Lactobacillus plantarum, and incubate at 35°C Ferment and cultivate for 3 days, heat to 85°C and sterilize for 18 minutes; (4) Filter the fermented rice milk emulsion with a plate basket to remove the coarse residue; (5) Concentrate the filtrate through a membrane fractional filtration, and then dry the concentrate under vacuum at room tempe...
Embodiment 2
[0014] (1) Weigh 5 kg of rice, break it into 80 mesh fineness, add 33 kg of drinking water, soak for 5 hours, heat to 80 ° C for 17 minutes to gelatinize; (2) add 35 g of starch to the gelatinized rice paste Enzyme, incubate at 45°C for 70 minutes to prepare amylase-treated rice milk; add 10 grams of protease to the amylase-treated rice milk, and incubate at 37°C for 50 minutes to prepare protease-treated rice milk; heat The protease-treated rice milk was kept at 85°C for 7 minutes to prepare rice milk; (3) after the temperature of the rice milk dropped to room temperature, inoculate the expanded and cultured Lactobacillus plantarum producing γ-aminobutyric acid, and inoculate at 32°C Ferment and cultivate for 4 days, heat to 90°C and sterilize for 10 minutes; (4) Filter the fermented rice milk emulsion with a plate basket to remove the coarse residue; (5) Concentrate the filtrate through a membrane fractionation filtration, and then vacuum-dry the concentrate at room temperatu...
Embodiment 3
[0017] (1) Weigh 8 kg of rice, break it into 90 mesh fineness, add 48 kg of drinking tap water, soak for 8 hours, heat to 80 ° C for 20 minutes; (2) add 120 grams of starch to the gelatinized rice paste Enzymes were incubated at 45°C for 90 minutes to prepare amylase-treated rice milk. Add 40 grams of protease to the amylase-treated rice milk, and incubate at 40°C for 65 minutes to obtain protease-treated rice milk; heat the protease-treated rice milk to 85°C for 8 minutes to obtain rice milk; 3) After the temperature of the rice milk drops to room temperature, inoculate the expanded and cultured Lactobacillus brevis producing γ-aminobutyric acid, ferment and cultivate it at 37°C for 4 days, and heat it to 90°C for 10 minutes to sterilize; (4) The fermented rice milk emulsion is filtered with a plate basket to remove the coarse slag, and then centrifuged to remove the slag; (5) The centrifuged liquid is concentrated through 5 times of membrane classification filtration, and th...
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