Three-dimensional isinglass multi-hole bracket and the preparing method

A porous scaffold and three-dimensional gelatin technology, which is applied in medical science, prosthesis, coating, etc., can solve the problems of plasticity, mechanical properties and microstructure that are difficult to meet the needs of tissue engineering

Inactive Publication Date: 2007-09-19
SHANGHAI TISSUE ENG LIFE SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its preparation method is often limited to the commonly used freeze drying method (freeze drying)
The plasticity, mechanical properties and microstructure of the porous gelatin scaffold materials prepared by the traditional freeze-drying method are difficult to meet the needs of tissue engineering

Method used

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  • Three-dimensional isinglass multi-hole bracket and the preparing method
  • Three-dimensional isinglass multi-hole bracket and the preparing method
  • Three-dimensional isinglass multi-hole bracket and the preparing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Preparation of three-dimensional gelatin porous scaffold material I

[0067] Put 1 g of gelatin into 100 ml of water, and magnetically stir for 60 min in a water bath at 30° C. to completely dissolve the gelatin in the water to prepare a 1% gelatin solution. Store at -4°C for later use.

[0068] Take 10ml of 1% gelatin solution and stir it magnetically in a water bath at 30°C. Gradually, 10ml of n-pentanol was added in portions to form a white emulsion. Take 0.5ml of 0.5% glutaraldehyde, stir it with magnetic force for 1min, pour it into a mold, and put it into a -20°C refrigerator to pre-freeze for 24h. The frozen gelatin is taken and dried in a freeze dryer to obtain a rough product of the porous gelatin scaffold material.

[0069] Soak the gelatin scaffold material with ethanol, and then rinse it with deionized water to remove organic solvents and residues. Freeze-dry again to obtain the three-dimensional gelatin porous scaffold material I. See attached picture ...

Embodiment 2

[0072] Preparation of three-dimensional gelatin porous scaffold II

[0073] Put 2 g of gelatin into 100 ml of water, stir magnetically in a water bath at 35° C. for 60 min to completely dissolve the gelatin, and prepare a 2% gelatin solution. Store at -4°C for later use.

[0074] Take 10ml of 2% gelatin solution and stir it magnetically in a water bath at 35°C. Gradually and in portions, 15 ml of n-propanol was added to form a white emulsion. Take 1ml of 0.5% glutaraldehyde, stir it magnetically for 2 minutes, pour it into a mold, and put it in a -20°C refrigerator for 24 hours. Get the frozen gelatin and dry it in a freeze drier to obtain a white porous material.

[0075] Soak the white gelatin porous material with ethanol, then soak and rinse with deionized water, remove free glutaraldehyde with 5% sodium borohydride, and wash with a large amount of deionized water to remove organic solvents and residues. Freeze-dry again to obtain the three-dimensional gelatin porous sc...

Embodiment 3

[0078] Preparation of Three-dimensional Gelatin Porous Scaffold III

[0079] Put 5 g of gelatin into 100 ml of water, and magnetically stir in a water bath at 37° C. for 60 min to completely dissolve the gelatin in the water to prepare a 5% gelatin solution. Store at -4°C for later use.

[0080] Take 10ml of 5% gelatin solution and stir it magnetically in a water bath at 37°C. Gradually, add 20ml of n-butanol in batches to form a white emulsion. Take 0.5ml of 1% glutaraldehyde, stir it magnetically for 3 minutes, pour it into a mold, and put it in a -25°C refrigerator for at least 24 hours. Get the frozen gelatin and dry it in a freeze dryer to obtain a white porous material.

[0081] Soak the white gelatin porous material with ethanol, then soak and rinse with deionized water, remove free glutaraldehyde with 5% sodium borohydride, and wash with a large amount of deionized water to remove organic solvents and residues. Freeze-dry again to obtain the three-dimensional gelat...

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Abstract

The invention discloses 3D gelatin porous scaffold material, with features of homogeneous pore size and distribution, easily forming, resisting compression and resisting bending. The invention also discloses a method of 3D gelatin porous scaffold material using latex freeze drying method. The said 3D gelatin porous scaffold material is especiallu adapted for skin tissue scaffold material.

Description

technical field [0001] The invention belongs to the field of biomaterials and tissue engineering, and relates to a porous film processed from natural high molecular substances and a preparation method thereof, which can be used as a scaffold material for skin tissue engineering. Background technique [0002] Natural polymer materials are the earliest medical materials used by humans. They have good biocompatibility, almost all of them are degradable, and the degradation products are non-toxic. Among tissue engineering scaffold materials, typical natural polymer materials include collagen, chitosan, gelatin, and sodium alginate. [0003] Gelatin is a partially denatured derivative of fibrous insoluble collagen, which is widely found in nature and is the main component of the extracellular matrix (ECM) in skin, bone and connective tissue. Gelatin consists of single chains of amino acids. From a structural point of view, gelatin molecules contain three-chain repeat units of g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/34A61L27/56
Inventor 赵莉曹谊林崔磊
Owner SHANGHAI TISSUE ENG LIFE SCI
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