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Preparation method of sesquiterpenoids Trichothec-9-en-4-o1,12,13-epoxy-,acetate,(4beta)-(8CI,9CI)

A technology of trichothec-9-en-4-ol, 13-epoxy-, which is applied in the field of microorganisms, can solve the problems of unsafe microbial source pesticides and other problems, and achieve the effect of excellent effect

Inactive Publication Date: 2007-09-26
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of endophytic fungus-Trichoderma harzianum strain L that isolates and screens out from the Chinese wolfberry tissue for the deficiency that chemical pesticides are unsafe and the types of microbial source pesticides are few; Another purpose of the present invention is to provide Using the strain L to ferment and extract its metabolites to prepare microbial pesticides for preventing and treating some plant fungal diseases and its preparation method

Method used

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  • Preparation method of sesquiterpenoids Trichothec-9-en-4-o1,12,13-epoxy-,acetate,(4beta)-(8CI,9CI)
  • Preparation method of sesquiterpenoids Trichothec-9-en-4-o1,12,13-epoxy-,acetate,(4beta)-(8CI,9CI)
  • Preparation method of sesquiterpenoids Trichothec-9-en-4-o1,12,13-epoxy-,acetate,(4beta)-(8CI,9CI)

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Embodiment 1

[0025] Embodiment 1: (isolation and screening of endophytic fungus Trichoderma harzianum strain L)

[0026] The present invention is obtained from Chinese wolfberry stems collected in Tianmu Mountain, Lin'an, Zhejiang Province in May 2006, through the steps of surface disinfection, separation, cultivation and fermentation of endophytic fungi, activity determination, and screening of effective bacterial strains for inhibiting plant fungal disease pathogens. Endophyte Trichoderma harzianum Rifai strain L, preserved.

[0027]Determination of the antibacterial activity of Trichoderma harzianum Rifai strain L: Take 1 mL of its fermentation supernatant in a sterile petri dish, mix it with 9 mL of PDA medium cooled to 50 ° C, and put it in each medium after cooling Place a 4mm-diameter bacteria cake for test bacteria such as Cucumber wilt, Bean anthracnose, Watermelon Fusarium, Cucumber blight and Tomato blight respectively, and the cake is connected to the center of the petri dish (...

Embodiment 2

[0030] Embodiment 2: (the preparation method of Trichoderma harzianum strain L fermentation metabolite)

[0031] Follow these steps:

[0032] (1) Activation culture on the slant of the strain test tube: the slant medium is potato dextrose medium (PDA), and its components and contents are: 200g of potatoes, 20g of glucose, and 20g of agar; first peel the potatoes, cut them into pieces and boil for half an hour , then filter with gauze, add glucose and agar, and make up water to 1L after dissolving; under sterile conditions, pick up a little bacteria of Trichoderma harzianum Rifai strain L with an inoculation needle, to a sterilized solid Put in a PDA medium test tube (15×150mm), put it in an incubator at 28°C±1°C, activate and cultivate for 96 hours, until the inclined surface of the test tube is covered with green spores;

[0033] (2) Seed liquid culture: seed medium is potato glucose liquid medium, and its component and content are: potato 200g, glucose 20g; After dissolvin...

Embodiment 3

[0037] Embodiment 3: (Utilize Trichoderma harzianum strain L fermentation metabolite to prepare into wettable powder)

[0038] in:

[0039] White carbon black: (produced by Shanghai Fuqi Chemical Co., Ltd.)

[0040] Nongdai 0203-B: (produced by Xingtai Lanxing Auxiliary Factory)

[0041] Sodium lignosulfonate: (produced by Shanghai Weicheng Chemical Co., Ltd.)

[0042] Light calcium carbonate: (produced by Shanghai Daewoo Biochemical Co., Ltd.)

[0043] The product contains 1.5% sesquiterpenoids Trichothec-9-en-4-ol, 12,13-epoxy-, acetate, (4β)-(8CI, 9CI), adding 2% white carbon black as an adsorbent, and Add 1% agricultural milk 0203-B and 3% sodium lignosulfonate auxiliary materials, and finally make up to 100% with light calcium carbonate. A").

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Abstract

The invention discloses a preparing method of active metabolite sesquiterpenes compound Trichothec-9-en-4-ol, 12, 13-epoxy-, acetate, (4beta)-(8CI, 9CI) of Chinese holly endogenetic fungus HaCi wood mildew strain L and appliance to prevent and cure mycosis of plant in microbe pesticide technical domain, which comprises the following steps: active-culturing bacterial; culturing seeds; proceeding ferment culture; extracting fermentate. This invention first utilizes ferment metabolite of Chinese holly endogenetic fungus into prevent and cure mycosis, which adds a new approach for agricultural bactericide.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the isolation of effective strains of plant endophytic fungi for preventing and controlling some plant fungal diseases, and the preparation and application methods of antibacterial metabolites thereof. Background technique [0002] There are a large number of endophytic fungi in plants. Endophytic fungi were first discovered in pastures, and later found in woody plants, herbs, grasses, mosses, lichens, seaweeds and other plants. So far, studies have shown that plant endophytic fungi can enhance the host's disease resistance, improve plant productivity, stress resistance and insect resistance, etc. These functions are due to the reproduction and growth of endophytic fungi in plants and the production of biologically active secondary metabolites in vivo. Therefore, endophytic fungi may become potential biological control agents in biological contro...

Claims

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Application Information

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IPC IPC(8): C12P17/18C12N1/14C12R1/645
Inventor 俞晓平申屠旭萍陈列忠
Owner CHINA JILIANG UNIV
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