Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Domesticated and selectively bred autoflocculating yeast mutant plant and its application

A self-flocculation and Saccharomyces cerevisiae technology, applied in the field of biotechnology and microorganisms, can solve the problems of difficult control, affecting the service life of immobilized yeast, and low sugar alcohol yield

Inactive Publication Date: 2007-10-03
DALIAN UNIV OF TECH
View PDF2 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, all kinds of immobilized yeast have the following disadvantages: 1. Consumption of carrier materials; 2. Once the fermentation process is contaminated by bacteria, it is difficult to control; 3. The internal fermentation of immobilized yeast produces CO 2 The cracking effect on it and the large amount of CO in the fermentation tank 2 The wear and tear caused by the disturbance seriously affects the service life of the immobilized yeast; 4. The by-product yeast in the ethanol fermentation process is difficult to recover, and the pure yeast can be obtained
However, the fusion strain SPSC01, as the first-generation strain selected and bred, cannot meet the requirements of industrial production in its flocculation stability and fermentation performance. During the operation of the fermentation device, the flocculation ability of SPSC01 is significantly reduced with the prolongation of the operation time. A corresponding increase in the number of free yeast cells in
More seriously, since one of its parent strains, S. pombe, was isolated from the pombe wine brewed by African natives, the by-products of the fermentation process had a high content of organic acids, resulting in a higher yield of sugar alcohols during the fermentation process of the fusion strain SPSC01. Low, only about 83% of the theoretical yield of 0.511 based on glucose

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Domesticated and selectively bred autoflocculating yeast mutant plant and its application
  • Domesticated and selectively bred autoflocculating yeast mutant plant and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Embodiment 1 is selected from the device of flocculating yeast strain

[0070] The device for breeding the self-flocculating yeast strain of the present invention is shown in Figure 2, and the device is a four-stage series fermenter system, which operates continuously.

[0071] In Fig. 2, the described device has: 1. WM-2A type oil-free air compressor; 2. Air rotameter (0-150mL / min); 3. Needle valve; 4. LP-3000pH indication and control System; 5. Temperature indication and control system 5; 6. Solenoid valve, whose specification is φ8mm; 7. Ammonia storage tank, whose specification is 250mL; 8. Constant temperature water tank; 9. Constant temperature water inlet; 10. Constant temperature water outlet; 11 1. Culture medium storage tank, which is a 2000mL triangular flask; 12. HL-2S type culture medium feeding peristaltic pump; 13. The total volume is about 1500mL (inner diameter φ110×height H150mm), and the working volume is about 1000mL (the bottom is 110mm upward. φ6m...

Embodiment 2

[0073] Breeding of embodiment 2 Saccharomyces cerevisiae mutant strain

[0074] 1. Preparation of starting strain and seed culture medium

[0075] One of the starting strains in this example is the Saccharomyces cerevisiae K2 strain commonly used in the ethanol fermentation industry. This strain has excellent ethanol fermentation performance (gifted by Dalian Distillery).

[0076] After the slant strains stored in the refrigerator at 4°C were activated at room temperature for 4 to 6 hours, they were inoculated into a 250mL Erlenmeyer flask containing 100mL of medium, and after shaking the flask at 30°C and 150rpm for 24 hours, they could grow as shown in Figure 2. Fermentation system inoculation.

[0077] The composition of shake flask culture medium is (g / L): glucose 10, yeast extract 5, peptone 3, prepared with ordinary tap water, and sterilized at 121° C. for 15 minutes.

[0078] The second starting strain of the present invention is SPSC01, and its cultivation method is ...

Embodiment 3

[0099] The preliminary detection of the flocculation performance of embodiment 3 bacterial strains

[0100] The self-flocculating yeast flo obtained in Example 1 was inoculated into the shake flask liquid medium, and cultured according to the same operation as the aforementioned yeast seed cultivation. After several hours, the shake flask was taken out, and the millimeter-sized self-flocculating granular yeast could be observed. After standing still for 2-3 minutes, the self-flocculating granule yeast almost all settled, and the culture medium was clear and transparent, which indicated that the yeast strain obtained by breeding had excellent flocculation performance. The self-flocculating yeast suspension cultured in shake flasks for 24 hours could be inoculated into the fermenter to investigate its Ethanol fermentation performance and flocculation stability.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The present invention belongs to the field of biological technology and microbiological technology, and discloses one kind of autoflocculating Saccharomyces cerevisiae, Saccharomyces cerevisiae flo, CGMCC No.1602; the use of the autoflocculating Saccharomyces cerevisiae in producing ethanol, fuel ethanol, etc; and the process of producing ethanol with the autoflocculating Saccharomyces cerevisiae. The autoflocculating Saccharomyces cerevisiae of the present invention has stable performance, and high yield of sugar alcohol, and can meet the requirement for industrial production.

Description

technical field [0001] The invention belongs to the field of biotechnology and microbiology technology, and in particular relates to a new breeding method of a Saccharomyces cerevisiae mutant strain with self-flocculation characteristics, the strain obtained by the breeding and its application. Background technique [0002] Fermentation is a key technology for the production of ethanol, especially fuel ethanol, which determines the consumption of raw and auxiliary materials and comprehensive energy consumption. The core of the fermentation technology is the strain. At present, the conventional Saccharomyces cerevisiae is used for ethanol fermentation at home and abroad. The strain is in a single-cell free state, suspended in the fermentation mash during the fermentation process, and continuously leaves the fermentation with the fermentation mash under continuous fermentation conditions system, resulting in a low cell density in the fermenter, the density of yeast cells per m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P7/06C12R1/865
CPCY02E50/17Y02E50/10
Inventor 白凤武袁文杰赵心清葛旭萌
Owner DALIAN UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com