Domesticated and selectively bred autoflocculating yeast mutant plant and its application

A self-flocculation, Saccharomyces cerevisiae technology, applied in the fields of biotechnology and microorganisms, can solve the problems of consuming carrier materials, increasing the number of free yeast cells, and being difficult to control.

Inactive Publication Date: 2010-05-12
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, all kinds of immobilized yeast have the following disadvantages: 1. Consumption of carrier materials; 2. Once the fermentation process is contaminated by bacteria, it is difficult to control; 3. The internal fermentation of immobilized yeast produces CO 2 The cracking effect on it and the large amount of CO in the fermentation tank 2 The wear and tear caused by the disturbance seriously affects the service life of the immobilized yeast; 4. The by-product yeast in the ethanol fermentation process is difficult to recover, and the pure yeast can be obtained
However, the fusion strain SPSC01, as the first-generation strain selected and bred, cannot meet the requirements of industrial production in its flocculation stability and fermentation performance. During the operation of the fermentation device, the flocculation ability of SPSC01 is significantly reduced with the prolongation of the operation time. A corresponding increase in the number of free yeast cells in
More seriously, since one of its parent strains, S. pombe, was isolated from the pombe wine brewed by African natives, the by-products of the fermentation process had a high content of organic acids, resulting in a higher yield of sugar alcohols during the fermentation process of the fusion strain SPSC01. Low, only about 83% of the theoretical yield of 0.511 based on glucose

Method used

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  • Domesticated and selectively bred autoflocculating yeast mutant plant and its application
  • Domesticated and selectively bred autoflocculating yeast mutant plant and its application
  • Domesticated and selectively bred autoflocculating yeast mutant plant and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Embodiment 1 is selected from the device of flocculating yeast strain

[0070] The device used for breeding the self-flocculating yeast strain of the present invention is as figure 2 As shown, the device is a four-stage series fermenter system, which operates continuously.

[0071] figure 2 Among them, the device has: 1. WM-2A oil-free air compressor; 2. Air rotameter (0-150mL / min); 3. Needle valve; 4. LP-3000 pH indication and control system ;5. Temperature indication and control system 5; 6. Solenoid valve, whose specification is φ8mm; 7. Ammonia storage tank, whose specification is 250mL; 8. Constant temperature water tank; 9. Constant temperature water inlet; 10. Constant temperature water outlet; 11. Medium storage tank, which is a 2000mL triangular flask; 12. HL-2S type medium feeding peristaltic pump; 13. The total volume is about 1500mL (inner diameter φ110×height H150mm), and the working volume is about 1000mL (there is a φ6mm at the bottom 110mm upward , ...

Embodiment 2

[0073] The breeding of embodiment 2 Saccharomyces cerevisiae mutant strains

[0074] 1. Preparation of starting strain and seed culture medium

[0075] One of the starting strains in this example is the Saccharomyces cerevisiae K2 strain commonly used in the ethanol fermentation industry. This strain has excellent ethanol fermentation performance (gifted by Dalian Brewery).

[0076] After the slant strains stored in the refrigerator at 4°C were activated at room temperature for 4 to 6 hours, they were inoculated into a 250mL Erlenmeyer flask filled with 100mL medium, and after shaking the flask at 30°C and 150rpm for 24 hours, they could be transferred to figure 2 Fermentation systems shown were inoculated.

[0077] The composition of shake flask culture medium is (g / L): glucose 10, yeast extract 5, peptone 3, prepared with ordinary tap water, and sterilized at 121° C. for 15 minutes.

[0078] The second starting strain of the present invention is SPSC01, and its cultivatio...

Embodiment 3

[0099] The preliminary detection of the flocculation performance of embodiment 3 bacterial strains

[0100] The self-flocculating yeast flo obtained in Example 1 was inoculated into the shake flask liquid medium, and cultured according to the same operation as the aforementioned yeast seed cultivation. After several hours, the shake flask was taken out, and the millimeter-sized self-flocculating granular yeast could be observed. After standing still for 2-3 minutes, the self-flocculating granule yeast almost all settled, and the culture medium was clear and transparent, which indicated that the yeast strain obtained by breeding had excellent flocculation performance. The self-flocculating yeast suspension cultured in shake flasks for 24 hours could be inoculated into the fermenter to investigate its Ethanol fermentation performance and flocculation stability.

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Abstract

The present invention belongs to the field of biological technology and microbiological technology, and discloses one kind of autoflocculating Saccharomyces cerevisiae, Saccharomyces cerevisiae flo, CGMCC No.1602; the use of the autoflocculating Saccharomyces cerevisiae in producing ethanol, fuel ethanol, etc; and the process of producing ethanol with the autoflocculating Saccharomyces cerevisiae.The autoflocculating Saccharomyces cerevisiae of the present invention has stable performance, and high yield of sugar alcohol, and can meet the requirement for industrial production.

Description

technical field [0001] The invention belongs to the field of biotechnology and microbiology technology, and in particular relates to a new breeding method of a Saccharomyces cerevisiae mutant strain with self-flocculation characteristics, the strain obtained by the breeding and its application. Background technique [0002] Fermentation is a key technology for the production of ethanol, especially fuel ethanol, which determines the consumption of raw and auxiliary materials and comprehensive energy consumption. The core of the fermentation technology is the strain. At present, the conventional Saccharomyces cerevisiae is used for ethanol fermentation at home and abroad. The strain is in a single-cell free state, suspended in the fermentation mash during the fermentation process, and continuously leaves the fermentation with the fermentation mash under continuous fermentation conditions system, resulting in a low cell density in the fermenter, the density of yeast cells per m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12P7/06C12R1/865
CPCY02E50/17Y02E50/10
Inventor 白凤武袁文杰赵心清葛旭萌
Owner DALIAN UNIV OF TECH
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