Method for producing epsilon-poly-L-lysine by reflux technique

A technology for lysine and lysine fermentation, which is applied in the field of ε-poly-L-lysine production by reflux process, can solve problems such as environmental pollution, and achieve the effects of reducing environmental pollution, reducing losses and improving yield

Inactive Publication Date: 2010-12-15
TIANJIN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In the existing ε-poly-L-lysine extraction process, the late penetration liquid and the late eluent are directly discharged without any measures for treatment or reuse, which is likely to cause environmental pollution

Method used

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  • Method for producing epsilon-poly-L-lysine by reflux technique
  • Method for producing epsilon-poly-L-lysine by reflux technique

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Embodiment 1 and Embodiment 2

[0018] What embodiment 1 and embodiment 2 described basically is existing production technology;

Embodiment 3 and Embodiment 5

[0019] What embodiment 3 and embodiment 5 described basically is existing circulation production process;

[0020] Embodiment 4 and Embodiment 6 are innovative processes compared with Embodiment 3 and Embodiment 5 respectively.

Embodiment 1

[0022] 5L fermenter, filled with 2.7L medium, inoculated with 300mL Streptomyces albicans seed culture solution, cultured at 30°C, when DO dropped to 30%, automatically controlled to 30%, aerobic culture for 96h, air flow rate 3L / min~7.5 L / min. When the pH drops to about 5.8, add ammonia water (25-28%) to maintain the pH at 5.8 until the residual sugar concentration in the fermentation broth drops to 10g / L by feeding the residual sugar concentration to maintain about 10g / L, and at the same time No longer controlling the pH let it drop to 4 naturally and keep it at 4. Feed: Glucose 800g / L, (NH 4 ) 2 SO 4 80g / L.

[0023] Cultivate for 96 hours, finish the fermentation, remove the bacteria and some solids by centrifugation, elute the supernatant with D152 type weakly acidic cation exchange resin, collect the eluate in the early stage, filter, concentrate in vacuum, and dry in a cyclone dryer to obtain ε- poly-L-lysine. The late eluent is reserved for later elution.

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Abstract

This invention refer to a method that using backstreaming technology to produce epsilon -poly-L-diaminocaproic acid. It uses blanc Streptomyces that screened from soil to fermentepsilon -poly-L-diaminocaproic acid[resist to S-(2-aminoethyl)-L-cysteine(abbreviate AEC below), Glycine, sulfaguanidine],then make the penetrated liquid from later stage of extracted procession by periodic duty,to fermentepsilon -poly-L-diaminocaproic acid; the penetrated liquid from later stage of extracted procession can be used for next elution. Decolorize the broth by negion exchange resin. This invention has changed the procedure of producing technology.Not only raise the yield ofepsilon -poly-L-diaminocaproic acid, save cost, but also reduce exhaustion of ferment liquid waste and hydrochloric acid liquid waste in elution, lessen environmental pollution; The decolor effect is enhanced and the lose is reduced becouse of decoloring the broth by negion exchange resin.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for producing ε-poly-L-lysine by a reflux process. Background technique [0002] ε-poly-L-lysine is a polypeptide containing 25 to 30 lysine residues secreted by Streptomyces albicans. It has good water solubility, easily soluble in water, slightly soluble in ethanol, but insoluble in ethyl acetate , ether and other organic solvents; broad antibacterial spectrum, in acidic and slightly acidic environment, it has inhibitory effect on Gram-positive bacteria and Gram-negative bacteria, the minimum inhibitory concentration is lower than 100μg / mL, but for mold and yeast The inhibitory concentration of bacteria is higher, the minimum inhibitory concentration is lower than 250μg / mL, and it also has inhibitory effect on heat-resistant bacillus and some pathogens. Under certain conditions, some phages can be significantly inactivated; high thermal stability , ε-poly-L-lysine will no...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/08C12N1/20C12R1/47
Inventor 贾士儒曹伟峰张恺瑞谭之磊孙艳芳李婷
Owner TIANJIN UNIV OF SCI & TECH
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