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Plants having increased yield and a method for making the same

A yield-increasing, plant-based technology applied in the field of molecular biology to achieve improved yield

Inactive Publication Date: 2008-01-23
CROPDESIGN NV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such selective breeding techniques have several drawbacks, namely that these techniques are generally labor-intensive and that the resulting plants often contain heterogeneous genetic components that, when passed from parent plants, do not necessarily produce desired trait

Method used

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  • Plants having increased yield and a method for making the same
  • Plants having increased yield and a method for making the same
  • Plants having increased yield and a method for making the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0150] Example 1: Gene cloning of AtSYT1, AtSYT2 and AtSYT3

[0151] The Arabidopsis AtSYT1 gene was amplified by PCR using an Arabidopsis seedling cDNA library (Invitrogen, Paisley, UK) as a template. After reverse transcription of the RNA extracted from the seedlings, the cDNA was cloned into pCMV Sport 6.0. The average insert size of the library is 1.5kb, and the number of original clones is in the order of 1.59×10 7 cfu. In 6×10 11 After the first amplification of cfu / ml, the original titer is determined to be 9.6×10 5cfu / ml. After extracting the plasmid, 200 ng template was used in 50 μl PCR mix. The primers used for PCR amplification include the AttB site recombined by Gateway, which is prm06681 (SEQID NO: 92; sense, the start codon is in bold, and the AttB1 site is in italics: 5'-GGGGACAAGTTTGTACAAAAAAGCAGGCTTAAACAATGCAACAGCACCTGATG-3') and prm06682 (SEQ ID NO: 92; ID NO: 93; antisense, complementary, AttB2 site is italicized: 5'-GGGGACCACTTTGTACAAGAAAGCTGGGTCATCATTAAGATTCC...

Embodiment 2

[0154] Example 2: Vector construction

[0155] Next, the entry clones p07466, p07467, and p07604 were used in the LR reaction together with the designated vector p00640 for rice transformation. This vector contains the following parts within the T-DNA boundary as functional elements: plant selectable marker; screenable Marker expression cassette; A Gateway expression cassette designed to recombine LR in vivo with the target sequence that has been cloned into the clone. The rice GOS2 promoter (SEQ ID NO: 89) (PR00129) for constitutive expression is upstream of this Gateway box.

Embodiment 3

[0157] Example 3: Evaluation and results of AtSYT1, AtSYT2 and AtSYT3 under the control of rice GOS2 promoter

[0158] Approximately 15 to 20 independent TO rice transformants were produced. The primary transformants were transferred from the tissue culture room to the greenhouse for growth and T1 seeds were harvested. Six events were retained, among which a 3:1 separation of the presence / absence of the transgene occurred in the T1 generation. By monitoring the expression of visual markers, about 10 T1 seedlings containing transgenes (heterozygous and homozygous) and about 10 T1 seedlings lacking the transgene (null zygote) were selected in each event.

[0159] Statistical analysis: F-test

[0160] Two-factor ANOVA (analysis of variation) was used as a statistical model for the overall evaluation of plant phenotypic characteristics. In all plants of all events transformed by the gene of the present invention, an F test was performed on all measured parameters. Perform an F test to...

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Abstract

The present invention relates to a method for increasing plant yield by modulating expression in a plant of a nucleic acid encoding a synovial sarcoma translocation (SYT) polypeptide or a homologue thereof. One such method involves introducing a SYT nucleic acid or variant thereof into a plant. The present invention also relates to transgenic plants into which a SYT nucleic acid or a variant thereof has been introduced, said plants having an increased yield relative to corresponding wild-type plants. The invention also relates to constructs useful in the methods of the invention.

Description

Invention field [0001] The present invention generally relates to the field of molecular biology, and relates to methods of increasing plant yield relative to corresponding wild-type plants. More specifically, the present invention relates to the translocation of encoding synovial sarcoma in plants ( sy novial sarcoma translocation (SYT) polypeptide or its homologue encoding nucleic acid expression method to increase plant yield. The present invention also relates to plants in which the expression of a nucleic acid encoding a SYT polypeptide or a homologue thereof is regulated, the plants having an increased yield relative to corresponding wild-type plants. The invention also provides constructs useful in the methods of the invention Background of the invention [0002] The ever-increasing world population and dwindling arable land available for agriculture have forced research to improve the efficiency of agriculture. Traditional crop and horticultural improvement methods use s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00
CPCC07K14/415C12N15/8261Y02A40/146
Inventor V·弗兰卡德
Owner CROPDESIGN NV
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