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Application of erigeron breviscapus methanol extracts in skin-whitening and speckle eliminating prevention

A technology of skin whitening and extraction, which is applied in the application field of methanol extract of scutellaria breviscapus, can solve the problems of weak penetration, high toxicity, and low activity

Inactive Publication Date: 2008-02-20
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these tyrosinase inhibitors have various disadvantages, such as low activity, high toxicity, weak penetration ability, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1——Extraction and preparation of breviscapine methanol extract

[0037] The methanol extract of scutellaria breviscapus can be prepared by the following method: 500g of dried whole branches of scutellaria breviscapus, soaked in methanol at room temperature for about 12 hours, after soaking and extraction, filtered, concentrated under reduced pressure, and dried into powder is scutellaria breviscapus methanol Extract.

Embodiment 2

[0038] Embodiment 2 - the inhibitory effect of methanol extract of breviscapine on purified mushroom tyrosinase:

[0039] Test method: Mushroom tyrosinase (Sigma, E.C. 1.14.18.1) was used to study the inhibitory activity of the powdered scutellaria extract prepared in Test Example 1. Dissolve tyrosinase in 1 / 15M phosphate buffer (pH 6.8) so that the concentration of the enzyme solution is 368U / ml. As a substrate, L-3,4-dihydroxyphenylalanine (L-DOPA) was dissolved in 1 / 15M phosphate buffer (pH 6.8) so that the concentration of L-DOPA was 5 mM / ml. The methanol extract of scutellaria breviscapus was prepared into 5 concentrations of 0, 125, 250, 500, 1000 and 2000 μg / ml with distilled water. All the above solutions are ready-to-use.

[0040] At 25°C, add 0.8ml of 5mM levodopa (L-DOPA), 1.2ml of 1 / 15M PBS (PH6.8) and 0.1ml of the test substance to the test tube successively, add an equal amount of distilled water to the negative control group, Incubate at 37°C for 5 min, then ...

Embodiment 3

[0050] Example 3-Inhibition of breviscapine methanol extract on tyrosinase activity in B16 mouse melanoma cells

[0051] Test method: B16 mouse melanoma cells (provided by the Key Laboratory of Transplantation Engineering and Transplantation Immunity of the Ministry of Health of Sichuan University), the cultured cells were digested and configured into a single cell suspension, and 1×10 5 / ml cell density seeded in a 6-well plate, put the 6-well plate into CO 2 Incubator, at 37°C, 5% CO 2 And under the condition of saturated humidity, after culturing for 24 hours, add 2ml of the culture solution containing the test substance, digest and collect the cells after 72 hours. Centrifuge at a frequency of 1000r / min for 5min with a centrifuge, wash twice with PBS, and add PBS to make a cell suspension. After counting the cell density, adjust the cell density to 1×10 6 / ml. Take 1ml of the cell suspension and centrifuge, discard the supernatant, add 1ml of 1% Triton X-100 (prepared ...

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PUM

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Abstract

The present invention discloses an application of methanol extract of breviscapine in the skin whitening and spot removing, which takes the breviscapine extract as the active ingredient to prepare the skin whitening or / and spot removing cosmetics, the medical supplies and foods for the inhibition of tyrosinase activity or / and melanin biosynthesis. The trials prove that the methanol extract of breviscapine can effectively inhibit the tyrosinase activity and the melanin formation without the side effects on the skin and whiten the tanned skin after the ultraviolet radiation, as well as prevent, improve and treat the age spots, freckles, red spots and other skin pigment deposition symptoms caused by the sun. The present invention explores a new usage of the methanol extract of breviscapine and has excellent application prospect.

Description

technical field [0001] The invention relates to the technical field of application of breviscapine methanol extract, and more specifically relates to the application of breviscapine flower methanol extract in skin whitening and spot-removing prevention and treatment. Background technique [0002] Human skin color is determined by the density and distribution of melanin in the skin, and is also affected by environmental or physiological factors, such as ultraviolet rays in the sun, fatigue, stress and genetic factors. The whitening methods widely used in existing cosmetics have two main whitening mechanisms: one is the melanin reduction mechanism, such as whitening with vitamin C, but the whitening effect is not very obvious; the other important mechanism is through the inhibition of tyrosin Whitening effect can be achieved by acid enzyme activity, such as hydroquinone, kojic acid, mulberry extract and arbutin (natural form of hydroquinone). However, the clinical effect of r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61K8/97A61Q1/06A61Q19/10A61Q19/04A61Q19/00A61P17/16A23L1/30A61K133/00A23L33/105A61K8/9789
Inventor 徐培渝黄毅娜熊静芳
Owner SICHUAN UNIV
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