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Method for preparing ethanol and yeast superoxide dismutase from sweet sorghum stem liquor

A technology for producing ethanol and superoxide, which is applied in the field of chemical engineering, can solve the problems of low yield, achieve high ethanol yield and reduce costs

Inactive Publication Date: 2008-04-30
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technology can only get one product of SOD, and the yield is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Measure the sterilized sweet sorghum stalk juice (total sugar content is 12%), add (NH) 2 SO 4 20g·L -1 , K 2 HPO 4 2.0g·L -1 , MgSO 4 ·7H 2 O 0.5g·L -1 , Zn(CH 3 COO) 2 2H 2 O5.0mg·L -1 , CuSO 4 ·5H 2 O 12mg·L -1 ; use 6mol L -1 Adjust the pH value to 4.0 with HCl aseptic solution; add yeast species—Saccharomyces cerevisiae according to 10% (v / v) of the total volume of the fermentation broth. At 30°C, 110r·min -1 Fermentation at a stirring rate. After ethanol fermentation, the fermentation liquid was heated at 5°C, 5000r min -1 After centrifugation for 15 min, the supernatant was used for distilling and extracting ethanol. Wash the precipitated bacteria once with sterile water, 3000r·min -1 Bacteria were collected by centrifugation at room temperature. Add 10% (w / w) of the weight of the wet thallus into the thallus activation solution, and place it in a water bath at 30°C for 120r·min -1 After shaking and activating for 60 min, destroy the yeast ...

Embodiment 2

[0045] Measure the sterilized sweet sorghum stalk juice (total sugar content is 12%), add (NH) 2 SO 4 25g·L -1 , K 2 HPO 4 2.5g·L -1 , MgSO 4 ·7H 2 O 0.65g·L -1 , Zn(CH 3 COO) 2 2H 2 O5.7mg·L -1 , CuSO 4 ·5H 2 O 12mg·L -1 ; use 6mol L -1 Adjust the pH value to 4.0 with HCl aseptic solution; add yeast species—Saccharomyces cerevisiae according to 10% (v / v) of the total volume of the fermentation broth. At 30°C, 130r·min -1 Fermentation at a stirring rate. After the fermentation, the fermented liquid was heated at 7°C, 3000r min -1 Centrifuge for 15 min, and take the supernatant for alcohol distillation. Wash the bacteria twice with sterile water, 3000r·min -1 Bacteria were collected by centrifugation at room temperature. Add 10% (w / w) of the weight of the wet thallus into the thallus activation solution, and place it in a water bath at 30°C for 120r·min -1 After shaking and activating for 30min, destroy the yeast cell wall by conventional methods, and add 1...

Embodiment 3

[0047] Measure the sterilized sweet sorghum stalk juice (total sugar content is 15%), add (NH) 2 SO 4 22.5g·L -1 , K 2 HPO 4 2.0g·L -1 , MgSO 4 ·7H 2 O 0.6g·L -1 , Zn(CH 3 COO) 2 2H 2 O5.0mg·L -1 , CuSO 4 ·5H 2 O 15mg·L -1 ; use 6mol L -1 The pH value of the HCl sterile solution is adjusted to 5.0; 15% (v / v) of the total volume of the fermentation broth is inserted into the yeast strain—Saccharomyces cerevisiae. At 35°C, 150r·min -1 Fermentation at a stirring rate. After the fermentation, the fermentation liquid was heated at 5°C, 5000r min -1 Centrifuge for 15 min, and take the supernatant for alcohol distillation. Wash the bacteria twice with sterile water, 3000r·min -1 Bacteria were collected by centrifugation at room temperature. Add 10% (w / w) of the weight of the wet thallus into the thallus activation solution, and place it in a water bath at 30°C for 120r·min -1 After shaking and activating for 60 min, destroy the yeast cell wall by conventional met...

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Abstract

The invention relates to a method for producing ethyl alcohol and yeast superoxide dismutase by using juice of stems of sweet sorghum, and belongs to the technical field of the biomaterial energy engineering. The invention has the following steps: firstly, the raw material of the juice of the stems of the sweet sorghum and fermentation auxiliary materials are taken to be sterilized and cooled forstandup; secondly, saccharomyces cervisiae is taken to be augmented stage by stage and cultured until the cell number per milliliter achieves above 1.0*10<8>; thirdly, the juice of the stems of the sweet sorghum and the fermentation auxiliary materials are mixed, and filled with the saccharomyces cerivisiae to perform ethyl alcohol fermentation; fourthly, after the ethyl alcohol fermentation is finished, refrigerated centrifugation to the fermentation liquid is performed, the supernatant fluid is taken to be used for distilling and extracting the ethyl alcohol, and the thallus is used for extracting the SOD; fifthly, after the collected thallus is activated, the cell wall of microzyme is damaged, and is heat insulated and extracted by phosphate buffer solution, then solid liquid separation is performed, and the obtained clear liquid is yeast SOD extracting solution. The yield rate of the ethyl alcohol can achieve 90.3 to 97.03 percent, and the output of the superoxide dismutase in each liter of fermentation liquid is 3.64*10<4>U to 6.95*10<4>U.

Description

technical field [0001] The invention relates to a method in the technical field of chemical engineering, in particular to a method for preparing ethanol and yeast superoxide dismutase from sweet sorghum stalk juice. Background technique [0002] Sweet sorghum is a C4 crop variety with high biomass, per hectare 2 Sweet sorghum can generally produce 2250-7500kg grain and 55-75t stalks rich in sugar (15°-20°Brix). The stalk sugar content is 15°-20°Brix, and the juice yield is above 55%. It is suitable for liquid fermentation technology to convert into ethanol for liquid fuel. It has broad prospects in the production of fuel ethanol and is known as the 21st century. "Green Energy". Relevant researchers have conducted ethanol fermentation conditions such as adding nitrogen source, nutrient salt, and initial sugar concentration of fermentation liquid, inoculum size, temperature, and number of shaker (stirring) revolutions to sweet sorghum stalk juice with the main goal of fermen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12R1/865
CPCY02E50/16Y02E50/10
Inventor 刘荣厚梅晓岩
Owner SHANGHAI JIAO TONG UNIV
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