Method for producing gellan gum finished product from gellan gum fermentation liquor

A technology of gellan gum and fermentation broth, applied in fermentation and other directions, can solve problems such as turbidity, inability to filter and clarify, and microfiltration membrane is easily damaged, and achieves the effect of reducing the amount of alcohol, low production cost, and good clarifying effect.

Inactive Publication Date: 2010-10-27
ZHEJIANG DSM ZHONGKEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the gellan gum content is very low, the fermentation broth has a high viscosity and cannot be filtered and clarified at room temperature. If it is filtered at high temperature, the impurities in the fermentation broth will turn against each other and make the color darker, so it is difficult to obtain transparent and colorless gellan gum after dissolution. Glue products
[0004] CN1351172A discloses a kind of technique from the transparent gellan gum of low acyl group in fermented liquid, the weak point of this process is: directly do deacyl treatment to fermented liquid, some organic matter (as amino acid, Sugar, pigment) react with each other to produce browning, the color of the reaction solution is very dark, even if the subsequent process uses activated carbon decolorization and microfiltration membrane filtration, it is difficult to remove, not to mention the high cost of high-temperature membrane filtration, and the microfiltration membrane is easily damaged at high temperature
[0006] CN1687437A discloses a method for extracting high acyl gellan gum. The method does not have a deacyl step, but the fermented liquid still needs to be subjected to high-temperature treatment in order to sterilize, and the purity of the product is very low due to the inability to remove the thallus fragments in the fermented liquid. It is cloudy after dissolving and can only be used as a low-end product
[0007] There is no report on the production technology of transparent high acyl gellan gum so far

Method used

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  • Method for producing gellan gum finished product from gellan gum fermentation liquor
  • Method for producing gellan gum finished product from gellan gum fermentation liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] A. Concentration process

[0078] 1. Add about 250L of 20% magnesium sulfate solution to the flocculation tank with 10m3 fermentation broth at normal temperature, and add 500L of 40% sodium hydroxide solution while stirring to obtain the flocculation fermentation broth.

[0079] 2. Use a pump to send the flocculation fermentation liquid to a plate and frame filter press for filtration and dehydration, and top wash with clean water, and unload to obtain 2000kg of gellan gum flocs.

[0080] 3. Feed the gellan gum floc into the desalination washing tank, add about 1m3 of 90% (v / v) isopropanol solution, stir and disperse, the concentration of isopropanol in the dispersion is about 50% (v / v), Use hydrochloric acid to adjust the pH to about 5.0, and after about 60 minutes, use a three-legged centrifuge to filter and remove the liquid to obtain about 1200 kg of crude gellan gum.

[0081] B. Redissolution process

[0082] 4. Add about 5m3 of water to the sol tank, heat up to ...

Embodiment 2

[0090] A. Concentration process

[0091] 1. Add about 15 m3 of 98% (v / v) isopropanol into a flocculation tank equipped with 10 m3 fermented liquid, stir evenly, and obtain the flocculated fermented liquid.

[0092] 2. Use a pump to send the flocculation fermentation liquid to the plate and frame filter press to filter and deliquify.

[0093] 3. Unload after top washing with a small amount of isopropanol to obtain 700kg of wet crude gellan gum.

[0094] B. Redissolution process

[0095] 4. Add about 3.5m3 of water to the sol tank, heat up to 80°C, add all the above-mentioned crude gellan gum, stir to dissolve, and obtain gellan gum turbid liquid.

[0096] 4a. Alkali treatment: Add lye to the gellan gum turbid solution while it is hot to make the pH of the gel solution 9.5-10.5, keep it for about 10 minutes, and then use acid solution to lower the pH to 6.0-8.0.

[0097] C. Clarification process

[0098] 5. While hot, use a vacuum drum machine pre-coated with diatomaceous ea...

Embodiment 3

[0104] With reference to the method of Example 1, the difference lies in steps 1-3:

[0105] 1. Add about 10m3 of 95% (v / v) ethanol to the flocculation tank equipped with 10m3 fermented liquid, stir evenly to obtain the flocculated fermented liquid.

[0106] 2. Use a pump to send the flocculation fermentation liquid to a plate and frame filter press to filter and deliquify, and unload to obtain 1200kg of wet crude gellan gum.

[0107] 3. Feed the crude product of gellan gum into a washing tank, add about 500 L of 90% (v / v) ethanol solution, stir and disperse, then filter and remove the liquid with a three-legged centrifuge to obtain about 700 kg of crude product of gellan gum. The product is a high acyl gellan gum product, recorded as 3-HA.

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Abstract

The invention relates to a method for obtaining transparent hydration inclined gellan gum products from gellan gum fermentation broth, comprising techniques of dehydration and condensation of the fermentation broth, repeated dissolution, clarification, and repeated dehydration and condensation and so on. After the great mass of pigments, residual sugar and metal ions in the fermentation broth areremoved by utilizing techniques of condensation and repeated dissolution of the invention, color and transparency of the gellan gum products are improved. The invention can use basically same technological line and production facilities to conveniently and alternately produce low acyl or high acyl gellan gum products according to market demand.

Description

technical field [0001] The invention relates to a method for producing gellan gum products from gellan gum fermentation liquid. Background technique [0002] According to the different gel characteristics of gellan gum caused by the acetyl group content in gellan gum polysaccharide molecules, gellan gum products are usually divided into two types: one is high acyl gellan gum products, which are easy to form viscoelasticity after hydration Gel, the base contained in normal gellan gum fermentation broth is high acyl gellan gum, the C of D-glucose residue in the repeating unit of its polysaccharide molecule 6 About 50% are substituted by acetyl groups; the other is a low-acyl gellan gum product, which is obtained by deacylation or partial deacylation of high-acyl gellan gum. After hydration, it is easy to form a brittle gel under the action of cations. According to the transparency of the gellan gum after hydration, it can be divided into transparent gellan gum and non-transpa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C12P19/04
Inventor 尹洪波陈亮
Owner ZHEJIANG DSM ZHONGKEN BIOTECH
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