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Preparation method of copper isomaltooligosaccharide

A technology of isomalto-oligosaccharide and isopropanol, which is applied in the field of preparation of isomalto-oligosaccharide copper, can solve the problems of poor absorption, toxicity and instability of inorganic copper, and achieves convenient operation, strong repeatability and practicability. high effect

Active Publication Date: 2011-12-21
HERBON INT POLYSACCHARIDE BIOTECH HEYUAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] It can be seen that copper has a very close relationship with human health and disease prevention, but because inorganic copper not only has poor absorption, but also is unstable and has certain toxicity, people are looking forward to a stable, low-toxic and efficient new chelated biological copper the advent of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Activated isomaltooligosaccharide (provided by Shenzhen Hanbang Polysaccharide Biotechnology Co., Ltd.) has an average molecular weight of 1000 and an intrinsic viscosity of 0.026. Weigh 100 grams of powder, add a small amount of distilled water, stir to dissolve, then increase the volume to 500ml, mix After uniformity, use a polarimeter to check the solution concentration (should be in the range of 18-20%), and the solution must be freshly prepared. Activated isomaltooligosaccharides can be obtained by fractional precipitation with ethanol or isopropanol solution or by gel filtration, which is a well-known technique.

[0017] The process of adopting ethanol solution to carry out step-by-step precipitation of isomaltooligosaccharides is as follows, the first step of precipitation: in 15-20% isomaltooligosaccharide solution, gradually add 95% ethanol, and stir at a slow speed (30 rpm points) until the concentration of ethanol in the solution is 38%, stop, let it stan...

Embodiment 2

[0027] (1) Activated isomaltooligosaccharide (provided by Shenzhen Hanbang Polysaccharide Biotechnology Co., Ltd.) average molecular weight 1500 (intrinsic viscosity 0.032), weigh 100 grams of this product, dissolve in water, 100 grams, dissolve in water, final volume 500ml , prepared on the same day.

[0028] (2) Separately weigh analytically pure copper sulfate (CuSO 4 ·5H 2 O) 49.0 grams, dissolved in water, and added to 400ml, prepared just before use.

[0029] (3)~(7) are identical with embodiment 1

[0030] (8) The complexed solution is put into a dialysis bag with a membrane hole of 500, and the pure water is dialyzed for 48 hours. The temperature should be lower than 20° C., and the purity of the solution is measured by sampling. The filter element filters and adjusts the elemental copper content in the solution to be 4.75%-5.25%, and the pH to be 6.0-7.0.

Embodiment 3

[0032] (1) Activated isomaltooligosaccharide (provided by Shenzhen Hanbang Polysaccharide Biotechnology Co., Ltd.) has an average molecular weight of 2000 (intrinsic viscosity 0.037), weighs 1000 grams of this product, dissolves it in pure water, and adjusts the volume to 5000ml, and stirs to make Dissolved and prepared on the same day.

[0033] (2) Weigh and take analytically pure copper acetate (CH 3 COO) 2 CU·H 2 O 393 grams, be dissolved in pure water, and adjust volume to 3000ml, stir and mix for subsequent use, prepare on the same day.

[0034] (3) Transfer the above two solutions to a 10L three-necked flask, and heat up to 30-35°C with a jacketed constant temperature heater.

[0035] (4)~(7) operation is the same as embodiment 1

[0036] (8) Dilute the above-mentioned complexed solution with 10 times the volume of pure water, filter it through a ceramic membrane, and then transfer it to a nano-membrane system for circulating operation to remove free iron and other s...

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Abstract

The invention belongs to the technical field of preparation of carbohydrate derivatives, in particular to a method for preparing copper isomaltooligosaccharides. The process includes: first, select and prepare specific molecular weight (1000, 1500 and 2000) non-toxic, harmless, and has a variety of biological functions and strong chemical reaction ability of activated isomaltooligosaccharides as the biological preparation of this product Precursor; second, the above-mentioned biological precursor and high-purity inorganic copper salt (such as copper chloride, copper sulfate and copper acetate, etc.) under the condition of pH10~10.5, carry out complexation step by step; the third, adopt comparative The perfect system separates and purifies the product, so that its quality, purity, stability and other indicators fully meet the requirements of the United States Pharmacopoeia and the international market.

Description

technical field [0001] The invention belongs to the technical field of preparation of carbohydrate derivatives, in particular to a method for preparing copper isomaltooligosaccharides. Background technique [0002] Copper is an essential trace element in the human body and is involved in many important life processes, including enzyme activation, energy production, blood formation and metabolism of many substances. It has important effects on the development of skin, hair, bones, brain and the function of heart, liver, central nervous system and immune system. Studies have shown that copper has a good effect on the prevention and treatment of many important diseases, such as anemia, tumors, cardiovascular diseases and influenza. [0003] Recently, many scientists put forward the "trace element theory of "mad cow" disease" on the basis of a large number of investigations and studies, and even determined that the onset of mad cow disease is closely related to the lack of copp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/02
Inventor 庄汉忠
Owner HERBON INT POLYSACCHARIDE BIOTECH HEYUAN CO LTD