Bacillus licheniformis and application thereof

A technology of Bacillus licheniformis, bl57cgmccno.2277, applied in the direction of bacteria, enzymes, biochemical equipment and methods, etc., can solve the problems of limited usage, unsatisfactory effect, and corresponding synchronous reduction of feeding costs, and achieve high value , Improve the effect of using value

Inactive Publication Date: 2008-07-09
北京欣博阳科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effect is not ideal
Because, by directly adding cellulose-rich plant processing by-products to the feed, although the cost of feed can be reduced, due to the low digestion and utilization rate of these plant processing by-products by animal

Method used

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  • Bacillus licheniformis and application thereof
  • Bacillus licheniformis and application thereof
  • Bacillus licheniformis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1, the acquisition of Bacillus licheniformis (Bacillus licheniformis) BL57 CGMCC No. 2277 and its culture condition detection

[0024] 1. Screening and identification of Bacillus licheniformis BL57 CGMCC №.2277

[0025] 1) Acquisition of Bacillus licheniformis BL57 CGMCC №.2277

[0026] One gram of soil was taken from three different locations of soil samples in Taichung County, Taiwan Province, and 10ml Trypticase was added to each TM Soy Broth (TSB culture fluid) (each liter contains 20.0g of tryptone (Tryptose), 1.0g of glucose (Dextrose), 2.0g of disodium phosphate (Disodium Phosphate), 1.0g of potassium nitrate (PotassiumNitrate) , using Mandels-Reese medium (per liter containing 1.0 g of peptone (Peptone), 10.0 g of carboxymethyl cellulose (CMC, Carboxymethyl cellulose), (NH 4 ) 2 SO 4 1.4g, Urea0.3g, K 2 HPO 4 2.0g, CaCl 2 .H 2 O0.4g, MgSO 4 .7H 2 O0.3g, FeSO 4 .7H 2 O5.0mg, MnSO 4 .H 2 O1.6mg, ZnSO 4 .7H 2 O1.4mg, CoCl 2 .7H 2 (2.0mg, ...

Embodiment 2

[0044] Example 2, Detection of Enzyme Activity Produced by Bacillus licheniformis BL57 CGMCC No. 2277

[0045] 1. Identification of carboxymethyl cellulase activity of Bacillus licheniformis BL57 CGMCC №.2277, and confirmation of the optimal pH value of the enzyme

[0046] 1) Preparation of enzyme solution samples produced by Bacillus licheniformis BL57 CGMCC №.2277

[0047] Bacillus licheniformis (Bacillus licheniformis) BL57 CGMCC №.2277 according to 10 9 The inoculation amount of cfu / L was inoculated in Mendels-Reese culture medium, and after culturing at 45°C for 24 hours, the cultured Bacillus licheniformis (Bacillus licheniformis) BL57 CGMCC №.2277 fermentation broth was centrifuged at 12,000rpm at 4°C for 10 minutes Afterwards, the supernatant was filtered with a filter membrane with a filter diameter of 0.45 μm to obtain an enzyme liquid (fermentation liquid supernatant), which was stored at -20°C.

[0048] 2) Carboxymethyl cellulase identification of the enzyme solu...

Embodiment 3

[0062] Embodiment 3, the application of Bacillus licheniformis (Bacillus licheniformis) BL57 CGMCC No. 2277 in the production of feed utilizing raw materials containing plant fiber

[0063] In this example, Bacillus licheniformis (Bacillus licheniformis) BL57 CGMCC No. 2277 is used to ferment sunflower disc powder, wheat stalk, mung bean stalk powder, millet stalk powder, sunflower stalk powder, corncob powder, corn stalk or distiller's grains as raw materials. Production feed, the specific method is as follows:

[0064] Use a 500ml beaker to fill the raw materials, weigh 10g of sunflower powder, wheat stalk, mung bean stalk powder, millet stalk powder, sunflower stalk powder, corncob powder, corn stalk or distiller's grains as raw materials, and add 10g to each beaker respectively. 6 cfu / g raw material Bacillus licheniformis (Bacillus licheniformis) BL57 CGMCC №.2277, and add 50ml of deionized water, after mixing evenly, adjust the pH value to 6.5, put it in a 45°C incubator,...

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PUM

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Abstract

The invention discloses a bacillus licheniformis and application. The bacillus licheniformis is BL57 CGMCC No.2277. The bacillus licheniformis BL57 of the invention can utilize industrial or agricultural waste which contains cellulose as raw material such as plant straw, leaf, other industrial or agricultural waste tissue, grain stillage and the like and transmit the cellulose in these raw materials into reduction sugar, which improves the utility value of the waste greatly.

Description

technical field [0001] The invention relates to a bacillus licheniformis and its application. Background technique [0002] Plant fiber substances such as cellulose, hemicellulose and lignin are commonly found in plants. Due to their own characteristics, if they are used as feed, their absorption and utilization rate is very low, so they are often discarded as garbage. [0003] Just take maize, an annual grass of the genus Zea, as an example. This crop is very hardy and can be cultivated except on extreme sandy and clay soils. Investigations show that for every 100 kilograms of corn produced, 17.5 kilograms of corn ears (corn husks) and corn silk, 100 kilograms of corn stalks and 41 kilograms of corn cobs will be produced. It can be seen from the calculation that the total amount of by-products such as corn stalks and corncobs is 158.5% of the main output, which is quite an astonishing figure. But in general, because the components of corn stalks and corncobs are difficult...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/42C12N9/28C12N9/56C12R1/10
Inventor 邱云棕干小英王昭闵张萍汪志阳徐庆霖程景章
Owner 北京欣博阳科技有限公司
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