Sea pumpkin saponin content determination method

Abstract

The invention discloses a method for determining holothurin content, which is characterized in that the method comprises the following steps of: hydrolyzing a sample with a trifluoroacetic acid aqueous solution, neutralizing the hydrolysate, filtering, adding water to fixed volume, adding 1-pheny-3-methy-5-pyrazolone derivative agent to carry out derivation reaction, determining the content of quinose in the derivatives by high-performance liquid chromatographic analysis, and calculating the holothurin content in the sample by multiplying the content of quinose by a conversion coefficient. The invention has the advantages of simple operation, high sensitivity, less sample consumption and good stability, and is suitable for content determination of glycosides of various sea cucumbers.

Description

technical field

[0001] The invention relates to a method for determining biologically active substances, in particular to a method for determining the content of sea cucumber saponins. Background technique

[0002] Sea cucumber saponin is an important biologically active substance in sea cucumber, and there are more than 100 kinds of sea cucumber saponins that have been discovered so far. The structure of saponins in sea cucumbers is complex and varies with different species of sea cucumbers and growth environments. Different species of sea cucumbers have different structures of saponins, and the same species of sea cucumber usually contains several or even dozens of saponins with similar structures and subtle differences. In addition, sea cucumber also contains a large amount of complex components such as protein, polysaccharide, fat, and trace elements, which will inevitably bring many difficulties to the determination of saponin content in sea cucumber.

[0003] The pat...