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Cyclohexenone extract of antrodia camphorata

A technology of extracts and compounds, applied in the field of novel compounds, can solve problems such as the publication of active ingredients, and achieve the effect of avoiding cell mutation and preventing cardiovascular diseases

Active Publication Date: 2008-07-23
GOLDEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it can be known from many current experiments that the extract of Antrodia camphorata has the effect of suppressing cancer (such as the aforementioned Chen (1995)), the research on what kind of active ingredients can achieve the effect of inhibiting tumor cells is still in the experimental stage, and there is no research yet. The specific active ingredients have been published, so if the extract can be further purified and analyzed to find out its real effective anti-tumor ingredients, it will be of great help to the treatment of human cancer

Method used

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  • Cyclohexenone extract of antrodia camphorata
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  • Cyclohexenone extract of antrodia camphorata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: 4-Hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2-ring Separation of hexenone

[0022] Put about 100 grams of Antrodia antrodia mycelia, fruiting bodies or a mixture of the two into a conical flask, add an appropriate proportion of water and alcohol (70% to 100% alcohol aqueous solution), and heat it at 20 to 25 ° C. Stir and extract for at least 1 hour, then filter through filter paper and a 0.45 μm filter membrane to collect the extract.

[0023] The previously collected Antrodia camphorata extract was analyzed using a high performance liquid chromatography (High Performance Liquid chromatography) with an RP18 chromatographic tube (column), and methanol (A) and 0.1% to 0.5% acetic acid aqueous solution (B ) as the mobile phase (the solution ratio is: 0-10 minutes, B ratio is 95%-20%; 10-20 minutes, B ratio is 20%-10%; 20-35 minutes, B ratio 10% to 10%; 35 to 40 minutes, the proportion of B is 10% to 95%), eluting at a speed of 1ml...

Embodiment 2

[0026] Example 2: Activity test of anti-breast cancer tumor cells in vitro

[0027] In order to further test the inhibitory effect of the compound found in Example 1 on tumor cells, this example will first take the isolated compound in Example 1 according to the anti-tumor drug screening mode of the National Cancer Institute (NCI) in the United States. 4-Hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone compound , adding MCF-7 and MDA-MB-231 human tumor cell culture medium to test the viability of tumor cells. The test of cell viability can be analyzed by the known MTT assay, and both MCF-7 and MDA-MB-231 are human breast cancer tumor cell lines.

[0028] MTT assay is a commonly used analytical method for analyzing cell proliferation, percent of viable cells, and cytotoxicity. Among them, MTT (3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide) is a yellow dye, which can be absorbed by living cells and absorbed by succinate tetra...

Embodiment 3

[0033] Example 3: Activity test for adjuvant therapy of breast cancer tumor cells in vitro

[0034] This test is also tested according to the National Cancer Institute's In Vitro Screening Model. First, human breast cancer cells MCF-7 and MDA-MB-231 were cultured in culture medium containing fetal calf serum for 24 hours, and the proliferated cells were washed once with PBS and washed with 1 times trypsin-EDTA Cells were treated followed by centrifugation at 1,200 rpm for 5 minutes to pellet the cells and discard the supernatant. Then add 10ml of new culture medium and shake gently to resuspend the cells. Before the test, first add 0.0017 μg / ml paclitaxel (Taxol) to treat the cells for 72 hours, then divide the cells into 96-well microplates, and then add 0 μg / ml (control group) to each well, 30, 10, 3 , 1, 0.3, 0.1 and 0.03 μg / ml of 4-hydroxyl-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2, 6,10-dodecatriene)-2-cyclohexenone (test group), at 37°C, 5% CO 2 Incubate for 48 hou...

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Abstract

The invention discloses a new compound and the usage, in particular to a 4-hydroxy-2, 3- dimethoxy -6-methyl-5 (3, 7, 11- trimethyl-2, 6, 10-dodecatrien) -2- cyclohexenone (4-hydroxy- 2, 3-dimethoxy -6- methy-5 (3, 7, 11-trimethyl -dodeca-2, 6, 10- trienyl) -cyclohex-2-enone) separated from antrodia camphorate extract and the application in inhibiting the growth of tumor cell. The new compound is firstly seen in antrodia camphorate. The 4-hydroxy-2, 3- dimethoxy -6-methyl-5 (3, 7, 11- trimethyl-2, 6, 10-dodecatrien) -2- cyclohexenone (4-hydroxy- 2, 3-dimethoxy -6- methy-5 (3, 7, 11-trimethyl -dodeca-2, 6, 10- trienyl) -cyclohex-2-enone) separated from antrodia camphorate extract and the application in inhibiting the growth of tumor cell has the advantages of inhibiting the growth of breast cancer, liver cancer and prostate cancer tumor cell, being a component of drug combination inhibiting the growth of the tumor cell at the same time or further being applied in preventing cardiovascular disease or being added into healthy food by right of antioxidant activity.

Description

technical field [0001] The present invention relates to a novel compound, in particular to a compound isolated and purified from the extract of Antrodiacamphorata and its application in inhibiting tumor cell growth. Background technique [0002] Antrodia camphorata (Antrodia camphorata), also known as Antrodia camphorata, Antrodia camphorata, Red camphor, Red Antrodia, Antrodia camphorata or Zhangku inner mushroom, is a unique fungus in Taiwan. Cinnamoum kanehirai Hay on the inner wall of the hollow decaying heartwood, so the fruiting body grows from the inner surface of the trunk. Cinnamomum camphora is currently mainly distributed in mountainous areas such as Taoyuan and Nantou. Since Cinnamomum camphora is an extremely rare conservation tree species in Taiwan, coupled with man-made illegal logging, the number of wild Antrodia camphorata that can grow parasitic on it is even rarer. The growth is rather slow and the growing season is only between June and October, making i...

Claims

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Application Information

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IPC IPC(8): C07C403/02A61K31/122A61K36/07A61P35/00
CPCC07C51/00A61P35/00A61P35/02A61P35/04
Inventor 刘胜勇温武哲邹宛玲郭茂田张君绶黄俊鸿李雅嫈霍嘉恒
Owner GOLDEN BIOTECH
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