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Method for extracting phycocyanin from phormidium tenue

A technology for slender algae and phycocyanin, which is applied in the field of extracting phycocyanin, can solve the problem of high cost, and achieve the effects of convenient operation, easy method and high yield.

Inactive Publication Date: 2010-09-15
CHINA UNIV OF GEOSCIENCES (WUHAN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the blue pigments used in food and other industries are mainly chemically synthesized pigments, while natural blue pigments are rarely used. Most of the domestic phycocyanin is extracted from spirulina and large seaweed, and their phycocyanin content is mostly higher than The slender mats are lower. In addition, the cost of cultivating macroalgae is much higher than the cost of cultivating slender mats

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] The specific implementation steps of extracting the phycocyanin of slender mats are as follows:

[0013] A. Preparation of culture medium

[0014] Add 1.5g of sodium nitrate, 0.04g of dipotassium hydrogen phosphate, 0.075g of magnesium sulfate, 0.036g of calcium chloride, 0.006g of citric acid, 0.006g of ferric ammonium citrate, 0.001g of disodium edetate, Sodium carbonate 0.02g, trace element solution 1ml. Add 286 mg of boric acid, 181 mg of manganese chloride tetrahydrate, 22.2 mg of zinc sulfate heptahydrate, 25.2 mg of sodium molybdate dihydrate, and 7.9 mg of copper sulfate pentahydrate into the trace element solution per 100 ml of distilled water, and stir well after completely dissolving.

[0015] B. Cultivation of slender algae

[0016] (1) First-level culture: insert the slender mat algae into a triangular flask (150-200ml) filled with culture medium, and culture it statically or with aeration. During aeration culture, adjust the airflow to 2.5L.min -1 , the...

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Abstract

The invention discloses a method for extracting phycocyanin from phormidium tenue, which comprises preparing sodium nitrate, dibasic potassium phosphate, magnesium sulfate, calcium chloride, citrate, ammonium iron citrate, disodium editate, sodium carbonate and micro element solution into phormidium tenue culture medium, arranging phormidium tenue into the culture medium to cultivate and prepare phormidium tenue powder, extracting and purifying phormidium tenue phycocyanin from the phormidium tenue powder. The invention has simple process, easy operation, high reliability, high yield and highquality, and the equipments are simple equipment and suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting phycocyanin from Matella slenderosa, belonging to the field of biotechnology. Background technique [0002] S. slenderis is a kind of filamentous cyanobacteria widely distributed in desert habitats. It belongs to Cyanophyta, Cyanophyta, Seglophyta, Oscillatoriaceae, and Schizophyta. At present, the utilization of Matella slenderis is generally limited to artificial algae crusts, and the development of other applications of Matsia slenderness is almost blank. The reason why the algae algae are used to form artificial algae crusts for desert control is mainly because it is rich in phycocyanin. Phycobiliproteins are a class of pigment complex proteins present in the phycobilisomes of some algae (mainly red and blue algae). Phycobiliprotein has strong fluorescence and emits orange-red fluorescence, but itself is red, purple or blue, so it is a colored polypeptide. According to the spectral characteristics...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/14
Inventor 谢作明刘永定王焰新沈银武胡春香
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)