Method for preparing sodium alginate/ chitosan sustained-release microcapsule by external gelation

A technology of sodium alginate and calcium chloride chitosan, which is applied in the preparation of microspheres, microcapsule preparations, pharmaceutical formulations, etc. In order to improve the drug content and encapsulation efficiency, narrow particle size distribution and good sphericity

Inactive Publication Date: 2008-08-13
ZHEJIANG CHINESE MEDICAL UNIVERSITY
View PDF1 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In addition, most of the current methods for removing the residual oil on the surface of the sustained-release microcapsules use organic solvents or surfactants. This method is difficult to completely remo

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing sodium alginate/ chitosan sustained-release microcapsule by external gelation

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0018] Example 1

[0019] Disperse 100 ml of doxorubicin (mass concentration 1.5%) sodium alginate (mass concentration 1.5%) physiological saline solution into 500 ml edible vegetable oil containing 0.5% span 80, and adjust the motor speed to 400 r / min and stir for 15 minutes. 500ml of 1% calcium chloride 0.5% chitosan (deacetylation degree 95.3%) solution was added to initiate the sodium alginate gelation reaction. After stirring for 30 minutes, the degreasing device adopts the precipitation self-drifting method to completely remove the slow Release the oil on the surface of the microcapsules. Collected and dried naturally to obtain doxorubicin sodium alginate chitosan sustained-release microcapsules. According to the 2005 edition of Pharmacopoeia of the People’s Republic of China, Appendix VA (Appendix 28) UV-Vis spectrophotometry, the drug loading of adriamycin in sodium alginate / chitosan sustained-release microcapsules was determined at 481nm, and the results were obtained The...

Example Embodiment

[0022] Example 2

[0023] Disperse 10 ml of 3% sodium alginate physiological saline solution into 80 ml of edible vegetable oil containing 0.5% span80, and adjust the motor speed to 1000 r / min and stir for 20 minutes. Add 50ml of 10% calcium chloride and 1% chitosan (85.7% deacetylation) solution by mass concentration to initiate the gelation reaction of sodium alginate. After stirring for 60 minutes, the sustained-release microcapsules are collected and passed The oil removal device adopts the precipitation and self-drifting method to completely remove the oil on the surface of the slow-release microcapsules, collect, and dry naturally to obtain the slow-release microcapsules.

Example Embodiment

[0024] Example 3

[0025] Disperse 10 ml of 1% sodium alginate physiological saline solution into 30 ml of edible vegetable oil containing 1% span 80, and adjust the motor speed to 500 r / min and stir for 15 minutes. Add 50ml of 3% calcium chloride and 0.5% chitosan (deacetylation degree 98.2%) solution with mass concentration respectively to initiate the gelation reaction of sodium alginate. After stirring for 20 minutes, the sustained-release microcapsules are collected and passed The oil removal device adopts the precipitation and self-drifting method to completely remove the oil on the surface of the slow-release microcapsules, collect, and dry naturally to obtain the slow-release microcapsules.

[0026] The oil removal device described in the above embodiment 1-3 is shown in Figure 1-3. It is composed of the oil removal tank 1, the sedimentation tank 2 and the oil collection tank 3, and its height is set from high to low from inside to outside. In the degreasing pool 1, there is...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for emulsionizing outer gelation and preparing sodium alginate/chitosan slow release micro-capsule in one step. The current preparation method of calcium alginate gel by internal gelation has defects that small parts of the calcium salt granules cannot be richly reacted with glacial acetic acid and remained in the sodium alginate calcium beads, the encapsulization materials are easily lost and the wrapping rate is low due to the calcium salt is difficult to evenly disperse. The invention uses the sodium alginate liquor as the disperse phase, the edible plant oil as the disperse medium and the sorbitan monooleate as the surfactant to emulsionize small liquid drops wrapped with oil; calcium chloride chitosan liquor is used for initiating the gelation reaction of the sodium alginate so as to generate sodium alginate/chitosan slow release micro-capsule; the oil remained on the surface is eliminated by a oil removing device with precipitation self-drifting method. The invention elevates the micro-capsule medicine contents and the wrapping rate.

Description

technical field [0001] The invention discloses a method for preparing sodium alginate / chitosan sustained-release microcapsules by one-step emulsification and external gelation. Background technique [0002] Sodium alginate / chitosan sustained-release microcapsules have good biocompatibility and mechanical strength, and are an excellent sustained-release drug delivery system, widely used in drug-controlled release carriers, gene delivery vehicles, immune isolation and artificial organs , cell culture microreactors, environmental purification and other aspects. At present, the preparation methods of sodium alginate / chitosan sustained-release microcapsules mainly include spraying method and emulsification method. Spray method mainly adopts coaxial air jet device and electrostatic titration device. The microcapsules prepared by this method have larger particle size, poorer microcapsule sphericity and smoothness, and the particle size of slow-release mic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K9/52A61K47/36B01J13/06
Inventor 范春雷沃兴德寿旭锋曲建全
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap