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DNA sensitivity electrode modified by hydrotalcite nanometer slice and preparation thereof

A sensitive electrode and hydrotalcite technology, applied in the direction of material analysis, instruments, and measuring devices through electromagnetic means, can solve the problems of small amount of DNA immobilization, easy leakage and anti-interference, and achieve strong anti-interference and wide linear response Range, effect of wide pH application range

Inactive Publication Date: 2010-11-17
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, electrochemical DNA sensors have problems such as small amount of DNA immobilization, easy leakage and poor anti-interference.

Method used

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  • DNA sensitivity electrode modified by hydrotalcite nanometer slice and preparation thereof
  • DNA sensitivity electrode modified by hydrotalcite nanometer slice and preparation thereof
  • DNA sensitivity electrode modified by hydrotalcite nanometer slice and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Preparation of hydrotalcite nanosheet sol

[0031] Weigh 0.12mol of Co(NO 3 ) 2 ·6H 2 O and 0.06mol of Al(NO 3 ) 3 9H 2Dissolve O in 100mL deionized water to make a salt solution; weigh 0.35mol of NaOH and dissolve it in 100mL deionized water to make an alkaline solution. Add the alkali solution and the salt solution into a colloid mill with a rotation speed of 400 rpm and mix them back for 2 minutes to obtain a pink suspension; quickly transfer the suspension to a four-necked flask, and crystallize at 100°C under the protection of nitrogen After 6 hours, filter with suction, wash until the pH of the filtrate is 6, then put the filter cake in an oven at 50°C to dry for 48 hours, and grind to obtain cobalt-aluminum hydrotalcite powder with a Co / Al molar ratio of 2:1. Take 0.025 g of the cobalt-aluminum hydrotalcite, add it to 50 mL of formamide, and stir at a constant temperature of 90° C. for 0.5 hour on a magnetic stirrer to obtain a hydrotalcite nanosheet so...

Embodiment 2

[0036] (1) Preparation of hydrotalcite nanosheet sol

[0037] Weigh the Co(NO 3 ) 2 ·6H 2 O and 0.04mol of Al(NO 3 ) 3 9H 2 Dissolve O in 100mL deionized water to make a salt solution; weigh 0.31mol of NaOH and dissolve it in 100mL deionized water to make an alkaline solution. Add the alkali solution and the salt solution into a colloid mill with a rotation speed of 700 rpm and mix them back for 1.5 minutes to obtain a pink suspension; quickly transfer the suspension to a four-necked flask, and crystallize at 90°C under nitrogen protection After 7 hours, filter with suction, wash until the pH of the filtrate is 6.5, then put the filter cake in an oven to dry at 70°C for 24 hours, and grind to obtain cobalt-aluminum hydrotalcite powder with a Co / Al molar ratio of 3:1. Take 0.05 g of the cobalt-aluminum hydrotalcite, add it to 50 mL of formamide, and stir at a constant temperature of 80° C. for 2 hours on a magnetic stirrer to obtain a hydrotalcite nanosheet sol with a con...

Embodiment 3

[0042] (1) Preparation of hydrotalcite nanosheet sol

[0043] Weigh 0.12mol of Co(NO 3 ) 2 ·6H 2 O and 0.03mol of Al(NO 3 ) 3 9H 2 Dissolve O in 100mL deionized water to make a salt solution; weigh 0.29mol of NaOH and dissolve it in 100mL deionized water to make an alkaline solution. Add the alkali solution and the salt solution into a colloid mill with a rotation speed of 1000 rpm and mix them back for 1 minute to obtain a pink suspension; quickly transfer the suspension to a four-necked flask, and crystallize at 95°C under the protection of nitrogen After 8 hours, filter with suction, wash until the pH of the filtrate is 7, then put the filter cake in an oven and dry at 80°C for 12 hours, and grind to obtain cobalt-aluminum hydrotalcite powder with a Co / Al molar ratio of 4:1. Take 0.1 g of the cobalt-aluminum hydrotalcite, add it to 50 mL of formamide, and stir at a constant temperature of 70° C. for 3 hours on a magnetic stirrer to obtain a hydrotalcite nanosheet sol ...

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Abstract

The invention relates to a DNA sensitive electrode decorated by hydrotalcite nano-chip and the preparation method thereof, which belongs to the field of electrochemical biosensors and preparation technology thereof. The sensitive electrode uses a glassy carbon electrode as the substrate electrode, and a sensitive film composed of double-strand DNA and co-aluminum hydrotalcite nano-chip is decorated on the surface of the substrate electrode. The preparation method of the sensitive electrode is as follows: the co-aluminum hydrotalcite nano-chip collosol is coated on the surface of the clean glassy carbon electrode; after dried at room temperature, the glassy carbon electrode is washed with second distilled water and dried, then the double-strand DNA solution is dropped on the surface of thehydrotalcite nano-chip; after dried at room temperature, the glassy carbon electrode is washed with second distilled water and dried, then the DNA sensitive electrode decorated by hydrotalcite nano-chip is prepared. The sensitive electrode has the advantages that the invention uses the properties of the hydrotalcite nano-chip, that is, the hydrotalcite nano-chip has large surface area, has high-density positive charge, has a lot of hydroxide radical on the surface and has good electric conductibility and so on, to realize the stability and fixing of mass DNA, and the electrode can be used in quantitative determination of phenol, which has good anti-interference performance.

Description

technical field [0001] The invention belongs to the technical field of electrochemical biosensors and preparation thereof, in particular to a DNA sensitive electrode modified by hydrotalcite nanosheets and a preparation method thereof. Background technique [0002] Electrochemical biosensors have the characteristics of stable performance, low price, easy operation, and easy miniaturization, and have been widely used in clinical diagnosis, industrial control, food testing, drug analysis, environmental analysis, biochips, and military fields. Among them, electrochemical DNA sensors have attracted much attention in recent years. They are not limited to the testing of gene sequences, but have also been greatly developed in drug analysis and pollutant testing. They are currently a hot spot in the field of electrochemical biosensor research. As in the literature (1) Biosensors and Bioelectronics, 2006, 21: 1777-1783, Kavita Arora et al. used the electrodeposition method to form a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
Inventor 杨文胜李立群王毅
Owner BEIJING UNIV OF CHEM TECH