Fluorescent carbon dot nanoprobe for detecting hydrogen sulfide and imaging hydrogen sulfide in living cells based on inner filter effect and its application method

A technology of fluorescent carbon dots and nanoprobes, which is applied in the field of biochemical analysis, can solve the problems of continuous detection of hydrogen sulfide content and achieve high selectivity and low cost

Active Publication Date: 2020-06-12
HENAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing fluorescent probes can only detect whether the sample to be tested contains hydrogen sulfide, but cannot continuously detect the content of hydrogen sulfide

Method used

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  • Fluorescent carbon dot nanoprobe for detecting hydrogen sulfide and imaging hydrogen sulfide in living cells based on inner filter effect and its application method
  • Fluorescent carbon dot nanoprobe for detecting hydrogen sulfide and imaging hydrogen sulfide in living cells based on inner filter effect and its application method
  • Fluorescent carbon dot nanoprobe for detecting hydrogen sulfide and imaging hydrogen sulfide in living cells based on inner filter effect and its application method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Preparation method of fluorescent carbon dot nanoprobes based on inner filter effect detection of hydrogen sulfide and hydrogen sulfide imaging in living cells:

[0034] 1. Synthesis of Fluorescent Carbon Nanodots

[0035] Fluorescent nano-carbon dots (CDs) were prepared by pyrolyzing tomatoes, as follows: Weigh 5.0 g of fresh tomatoes in a mortar, grind them thoroughly and filter them through a 150-mesh filter membrane, take 3 mL of the filtrate in a hydrothermal reaction kettle, and then add 20mL of ultrapure water was heated to 250°C and stirred. After 10 hours, stop heating and naturally cool to room temperature, filter, and take the filtrate in a dialysis bag (MWCO: 1 kDa, pore size: ca. 1.0 nm) under the environment of ultrapure water Dialyze for 24 hours in the dark, and store the supernatant at 4°C in the dark. figure 1 The transmission electron micrograph and particle size distribution diagram of the synthesized green carbon quantum dots are given. It can be s...

Embodiment 2

[0043] 1. Synthesis of Fluorescent Carbon Nanodots

[0044] Fluorescent nano-carbon dots (CDs) were prepared by pyrolyzing tomatoes, as follows: Weigh 5.0 g of fresh tomatoes in a mortar, grind them thoroughly and filter them through a 150-mesh filter membrane, take 3 mL of the filtrate in a hydrothermal reaction kettle, and then add 21mL of ultrapure water was heated to 250°C and stirred. After 10 hours, stop heating and naturally cool to room temperature, filter, and take the filtrate in a dialysis bag (MWCO: 1 kDa, pore size: ca. 1.0 nm) under the environment of ultrapure water Dialyze for 24 hours in the dark, and store the supernatant at 4°C in the dark.

[0045] 2. Synthesis of DMI

[0046] 2,3,3-Trimethyl-3H-indole (1.6g, 10mmoL) and iodomethane (2.84g, 20mmoL) were mixed and dissolved in 10mL of acetonitrile, refluxed at 60°C for 11h under the protection of argon, and naturally cooled to room temperature , a light pink precipitate was obtained, filtered, washed three...

Embodiment 3

[0052] 1. Synthesis of Fluorescent Carbon Nanodots

[0053] Fluorescent nano-carbon dots (CDs) were prepared by pyrolyzing tomatoes, as follows: Weigh 5.0 g of fresh tomatoes in a mortar, grind them thoroughly and filter them through a 150-mesh filter membrane, take 3 mL of the filtrate in a hydrothermal reaction kettle, and then add 22mL of ultrapure water was heated to 250°C and stirred. After 10 hours, stop heating and naturally cool to room temperature, filter, and take the filtrate in a dialysis bag (MWCO: 1 kDa, pore size: ca. 1.0 nm) under the environment of ultrapure water Dialyze for 24 hours in the dark, and store the supernatant at 4°C in the dark.

[0054] 2. Synthesis of DMI

[0055] 2,3,3-Trimethyl-3H-indole (4.8g, 30mmoL) and iodomethane (8.52g, 60mmoL) were mixed and dissolved in 10mL of acetonitrile, refluxed at 60°C for 11h under the protection of argon, and naturally cooled to room temperature , a light pink precipitate was obtained, filtered, washed three...

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Abstract

The invention proposes a fluorescent carbon dot nano-probe for detecting hydrogen sulfide and imaging hydrogen sulfide in living cells based on the inner filter effect, and a using method thereof. A simple green method for fluorescence detection of hydrogen sulfide and tracing of hydrogen sulfide in living cells is established. Fluorescent carbon dots are prepared from tomatoes, the carbon dots (CDs) have strong green fluorescence at 520 nm under the irradiation of 410 nm ultraviolet light, red shift of the fluorescence emission peak gradually occurs with the increase of the wavelength of theexcitation light, the relative fluorescence quantum yield is 53%, the carbon dots acting as a fluorescence donor and an organic small-molecular receptor (DMI) form the probe based on the inner filtereffect, and the probe is used for detecting exogenous and endogenous hydrogen sulfide. A sensing system is successfully applied to hydrogen sulfide detection and live cell imaging analysis as the fluorescent probe, and a sensing method has broad application prospects in the fields of environmental analysis, biochemical analysis and cell imaging analysis.

Description

technical field [0001] The invention relates to the technical field of biochemical analysis, in particular to a fluorescent carbon dot nanoprobe for detecting hydrogen sulfide based on an inner filter effect and imaging hydrogen sulfide in living cells and a method for using the same. Background technique [0002] Carbon quantum dots (CDs), also known as carbon dots or carbon nanodots, are a new class of carbon nanomaterials, generally below 10nm in size, with excellent optoelectronic properties, uniform particles, good dispersion and low preparation costs, low It has the characteristics of high toxicity, good biocompatibility, adjustable excitation and emission wavelengths, good photostability, and no light flicker phenomenon. Compared with traditional metal quantum dots, CDs have many advantages, and their unique luminescent properties and biocompatibility have good application prospects in the fields of light-emitting devices, photocatalysis, biochemical analysis, optoele...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/65C09K11/02G01N21/64
CPCC09K11/025C09K11/65G01N21/64
Inventor 张旭陈云云方东张超杨婷周航
Owner HENAN UNIVERSITY
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