Process for producing eutrit by waste xylose mother liquor or eutrit mother liquor in production process of eutrit

A technology of xylose mother liquor and xylitol, which is applied in the field of xylitol production, can solve the problems of reducing the economic benefits of xylose, and achieve the effects of improving production economic benefits, simple and reasonable process, and reducing production costs

Inactive Publication Date: 2008-10-15
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have only utilized the isolated xylose or xylitol, but have not utilized the other larger content of arabinose (

Method used

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  • Process for producing eutrit by waste xylose mother liquor or eutrit mother liquor in production process of eutrit
  • Process for producing eutrit by waste xylose mother liquor or eutrit mother liquor in production process of eutrit

Examples

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Effect test

example 1

[0027] The xylose mother liquor (the sugar refractive concentration is about 63%, which contains 22% arabinose, 50% xylose, 6% galactose and 15% glucose) utilizes 732Na + The separation conditions are as follows: separation temperature 70°C, system pressure 1.1MPa, feed flow rate 1L / h, elution water flow rate 3.5L / h, separate arabinose from the xylose mother liquor, and obtain a purity of 95% arabinose liquid. Add this arabinose solution into the hydrogenation reactor, react until the reducing sugar content is less than 0.1%, and obtain the D-arabitol solution with a refractive concentration of 50% and a D-arabitol content of about 94%, ready for use .

[0028] Gluconobacter oxydans ATCC621 was inoculated in a 250mL Erlenmeyer flask containing 50mL of bacterial culture medium A, cultured on a rotary shaker at 30°C and 220rpm for 72h, the cells were collected by centrifugation, and washed once with 0.1M phosphate buffer before use. The composition of bacterial medium A is: 50...

example 2

[0032] Gluconobacter oxydans ATCC621 was inoculated in a 250mL Erlenmeyer flask containing 50mL bacterial culture medium B, and cultured on a rotary shaker at 37°C and 220rpm for 30h. The cells were collected by centrifugation and washed once with 0.1M phosphate buffer before use. The composition of bacterial medium B is: 30g / L glucose, 5g / L yeast extract, 10g / L peptone, 20g / L D-arabinitol.

[0033] The cells after washing are inserted into 50mL sugar refraction concentration as 10% xylitol crystallization mother liquor with the addition amount of 15% (W / V), and wherein xylitol content is 60%, and arabitol content is 30% (D - the concentration of arabitol is 30g / L), and the transformation is carried out on a rotary shaker at 25° C. and 220 rpm for 35 hours. At the end of the transformation, the concentration of xylitol in the transformation liquid is 88 g / L.

[0034] Centrifuge the transformed liquid to obtain 47 mL of supernatant, add 0.94 g of activated carbon, adjust the pH...

example 3

[0036]Acetobacter was inoculated in a 250mL Erlenmeyer flask containing 50mL bacterial culture medium C, cultured on a rotary shaker at 25°C and 220rpm for 50h, the cells were collected by centrifugation, washed once with 0.1M phosphate buffer and set aside for use. The composition of bacterial culture medium C is: 10g / L glucose, 20g / L yeast extract, 8g / L peptone, 30g / L D-arabinitol.

[0037] Mix 5 mL of the D-arabitol solution prepared in Example 1 with 10 mL of the xylitol crystallization mother liquor with a sugar refractive concentration of 20% (wherein the xylitol content is 60%, and the arabitol content is 30%), and add 0.5 g of glucose was distilled to 50 ml as a transformation solution (the content of D-arabitol was 32 g / L).

[0038] The washed cells were added into 50 mL of the above-mentioned transformation solution at an addition amount of 5% (W / V), and transformed at 37° C. and 220 rpm on a rotary shaker for 20 hours. At the end of the transformation, the concentra...

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Abstract

The invention discloses a method for producing xylitol through using xylose mother liquor or xylitol mother liquor depleted during the production of xylitol. The method comprises the following: 1 a step of adding hydrogen into arabinose separated from the xylose mother liquor to obtain D-arabitol or separating after adding hydrogen to obtain the D-arabitol; 2 a step of converting the D-arabitol obtained during the step 1 or the D-arabitol in the xylitol mother liquor into xylitol through making use of a strain simultaneously containing D-arabitol dehydrogenase and xylitol dehydrogenase; 3 a step of concentrating and crystallizing the xylitol conversion liquor obtained during the step 2 to obtain an xylitol product after removing thallus cells. The method which makes full use of the xylose mother liquor and xylitol mother liquor produced by a traditional method with simple and reasonable process can improve the utilization factor of raw materials such as corn cob and bagasse by about 9 to 14 percent, thereby greatly reducing the total production cost and consequently improving the economic efficiency of xylitol production.

Description

technical field [0001] The invention relates to a preparation process of xylitol, in particular to a method for efficiently utilizing waste materials in xylitol production to produce xylitol so as to improve the yield of xylitol, and in particular to utilizing waste xylose in xylitol production A method for producing xylitol from mother liquor or xylitol mother liquor. Background technique [0002] Xylitol is a natural five-carbon sugar alcohol and one of the important functional food additives. The sweetness of xylitol is 1.05 times that of sucrose, and its calories are equivalent to that of sucrose. Its metabolism does not require insulin, and it can replace sucrose as a sweetener for diabetics. Xylitol is not fermented by bacteria and is used in chewing gum as a sweetener to maintain oral acid-base balance and prevent dental caries. Studies have shown that xylitol can prevent the combination of bacteria and human cells and prevent respiratory infections; xylitol can als...

Claims

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Application Information

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IPC IPC(8): C12P19/02C12N1/20C12R1/02C12R1/01
Inventor 徐虹朱宏阳李莎蔡恒欧阳平凯
Owner NANJING UNIV OF TECH
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