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Production process of docosahexenoic acid grease by bioenzyme method wall-breaking

A technology of docosahexaenoic acid and biological enzyme method, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of affecting oil quality, chemical reagent residues, poor quality of crude oil, etc., Achieve the effect of preventing oxidation and breaking the wall completely

Inactive Publication Date: 2008-11-19
罗盖特生物营养品(武汉)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, there will be chemical reagent residues in the oil, which will affect the quality of the oil.
Moreover, the quality of crude oil is poor, and the yield after refining is only 30-50% of crude oil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A method for the production of docosahexaenoic acid oil by breaking the wall of a biological enzyme method: liquid shake flask culture, expanded liquid shake flask culture, primary seed cultivation, and secondary seed cultivation using Cryptidinium kotsuii slant bacteria and three-stage fermentation culture to obtain a fermented liquid. Add 0.2 kg of alkaline protease (2709) to 100 kg of fermentation broth, raise the temperature to 50-70° C., heat and stir for 3-9 hours, add 30 kg of 95% ethanol, add 150 kg of n-hexane, and extract to obtain docosahexaenoic acid Crude oil 3kg. Docosahexaenoic acid crude oil was subjected to hydration, alkali refining, decolorization and deodorization to obtain 2.34 kg of docosahexaenoic acid refined oil.

Embodiment 2

[0017] A method for the production of docosahexaenoic acid oil by breaking the wall of a biological enzyme method: liquid shake flask culture, expanded liquid shake flask culture, primary seed cultivation, and secondary seed cultivation using Cryptidinium kotsuii slant bacteria and three-stage fermentation culture to obtain a fermented liquid. Add 0.35 kg of neutral protease to 100 kg of fermentation broth, raise the temperature to 50-70° C., heat and stir for 3-9 hours, add 30 kg of 95% ethanol, add 150 kg of n-hexane, and extract to obtain 3 kg of docosahexaenoic acid crude oil . Docosahexaenoic acid crude oil was subjected to hydration, alkali refining, decolorization and deodorization to obtain 2.34 kg of docosahexaenoic acid refined oil.

Embodiment 3

[0019] A method for the production of docosahexaenoic acid oil by breaking the wall of a biological enzyme method: liquid shake flask culture, expanded liquid shake flask culture, primary seed cultivation, and secondary seed cultivation using Cryptidinium kotsuii slant bacteria and three-stage fermentation culture to obtain a fermented liquid. Add 0.45 kg of papain to 100 kg of fermentation broth, raise the temperature to 50-70° C., heat and stir for 3-9 hours, add 30 kg of 95% ethanol, add 150 kg of n-hexane, and extract to obtain 3 kg of docosahexaenoic acid crude oil. Docosahexaenoic acid crude oil was subjected to hydration, alkali refining, decolorization and deodorization to obtain 2.34 kg of docosahexaenoic acid refined oil.

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Abstract

The invention relates to a method for making docosahexaenic acid (DHA) grease through adopting bio-enzyme method wall breaking, comprising the following steps that: Crypthecodinium cohnii(Seligo)Javornicky is taken as starting strain; alga cell is obtained through carrying out continuous cultivation of Crypthecodinium cohnii(Seligo)Javornicky in liquid medium; and then DHA grease is extracted from the alga cell through bio-enzyme method wall breaking. The method has the advantages that: alga cell wall breaking is complete with the wall-breaking rate reaching more than 95 percent; the method does not need a drying step, and can prevent the oxidation of DHA crude oil, thereby ensuring that the peroxide value of extracted grease is extremely low (i.e. the POV value is less than 0.5); moreover, the yield of the extracted grease is more than 90 percent of total oil, while the refining yield is more than 70 percent of crude oil.

Description

technical field [0001] The invention relates to the technical field of using Crypthecodinium cohnii (Seligo) Javornicky to produce docosahexaenoic acid (DHA) oils, in particular to a biological enzyme method for breaking walls of docosahexaenoic acid Production method of oleic acid oil. Background technique [0002] In recent years, the important role of polyunsaturated fatty acids (PUFAs) on human health has been paid more and more attention. Among them, docosahexaenoic acid is favored by people because of its important physiological functions in humans and animals. Studies have shown that DHA is the basic component of cell membranes in certain tissues of the human body; it plays an important role in the development of the visual system and nervous system of infants; it also has the effect of lowering cholesterol, anti-coagulation and inhibiting cancer, preventing senile dementia disease, etc., and it is a fatty acid necessary for the growth of juvenile fish. The product...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12N1/12C12R1/89
Inventor 过群
Owner 罗盖特生物营养品(武汉)有限公司
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