Liver perfusion method for small-sized animals liver lymphocyte separation

Abstract

The invention discloses a hepatic perfusion method used for separating liver lymphocytes of small animals, which is characterized in that after the animal is killed the perfusion is carried out by the vena cava posterior; the method of the invention has simple and quick operation, sufficiently removes the circulated blood, requires no special equipments, has low experiment cost, achieves the ideal hepatic perfusion effect, and is suitable for separating the liver lymphocytes of small animals such as rats, etc. for most laboratories.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a liver perfusion method for isolating small animal liver lymphocytes. Background technique [0002] Normal liver tissue is supplied by arteries and portal veins, and 70%-85% of the blood supply comes from the portal vein. The portal vein collects blood from the stomach, small intestine, large intestine (except the back of the rectum), pancreas, and spleen, enters the liver through the hepatic portal, repeatedly branches into the sinusoid space in the liver, and finally gathers several hepatic veins into the posterior vena cava. [0003] Hepatic lymphocytes are closely related to liver diseases, and may be involved in the occurrence and development of many liver diseases such as hepatitis, cirrhosis, and liver cancer. In addition, they are also related to autoimmune diseases and organ transplant immunity. In order to study the phenotypic changes and significance of liver lymphocytes in t...

AI Technical Summary

Problems solved by technology

Portal vein hepatic perfusion is commonly used in foreign countries to exclude the influence of passenger lymphocytes, but this technique is difficult to operate, especially when the experimental animals are small animals such as mice, because the portal vein is very thin and the anatomical structure is deep, it needs Carefully peeling off the surrounding ligaments is very difficult to operate, and the puncture needle is not easy to fix, and it is easy to fall off and puncture the portal vein. Often, the puncture needle penetrates into the lobular branch veins, resulting in blind spots of different sizes in each lobe of liver (that is, those with residual blood) region), resulting in insufficient removal of circulating blood, and passenger lymphocytes mixed with the liver lymphocytes obtained in the subsequent separation, thus significantly affecting the accuracy of the experimental results; in addition, the portal vein hepatic perfusion method also requires a constant flow pump Perfusion devices and surgical microscopes, etc., the operation is cumbersome and time-consuming

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