Method for preparing drosophila melanogaster antimicrobial peptide

An antibacterial peptide, Drosophila technology, applied in the biological field, can solve the problems of small Drosophila individuals, underdeveloped economic value, and little research.

Inactive Publication Date: 2008-12-31
SHANGHAI HIGH SCHOOL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for a long time, people's research on Drosophila tends to focus on genetics, and Drosophila is small, so its economic value has not been deeply developed, and there are few domestic researches on it.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1 induces antimicrobial peptides with Staphylococcus aureus

[0015] Prepare beef extract medium and sterilize at 121°C for 20 minutes. Escherichia coli and Staphylococcus aureus were inoculated on the slant of beef extract medium by streaking method, and cultivated at 37°C for 24h.

[0016] Take 1 Staphylococcus aureus slant, add 5ml of normal saline (0.9% NaCl solution) respectively, scrape the thalline gently with an inoculation needle, move it into a sterile test tube, shake fully, and make a Staphylococcus aureus suspension.

[0017] Acupuncture method: Drosophila adults were anesthetized with ether, dipped in Staphylococcus aureus suspension with a capillary needle, and then needled the abdomen of Drosophila. Drosophila after stabbing continued to be cultured at 25°C for 24h.

Embodiment 2

[0018] Embodiment 2 induces antimicrobial peptides with Penicillium

[0019] Prepare PDA medium and sterilize at 121°C for 20 minutes. Penicillium was inoculated on the slant of PDA medium by streaking method, and cultured at 28°C for 4-5 days.

[0020] Culture method: Drosophila adults were anesthetized with ether, transferred to a culture bottle covered with Penicillium, and cultured at 25°C for 4-5 days.

Embodiment 3

[0021] The extraction of embodiment 3 antimicrobial peptides

[0022] Take the adult fruit flies induced in Examples 1 and 2 respectively, anesthetize the adult fruit flies with ether, weigh them, add Tris-HCl buffer solution at a ratio of 20:1, and grind the fruit flies to a pulp. The extract was centrifuged at 3500r / min for 10min, and the supernatant was stored at 4°C.

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PUM

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Abstract

The invention discloses a preparation method for drosophila cecropin, which induces the drosophila adults with bacteria or epiphyte and through acupuncture and culture to produce cecropin; the cecropin is then extracted to get the drosophila cecropin. The obtained cecropin has inhibiting effects on bacteria and epiphyte and has thermal stability. The natural cultured drosophila adults have no cecropin inside the body; the method can be used to induce the drosophila to generate the cecropin which has inhibiting effects on bacteria and epiphyte.

Description

technical field [0001] The invention relates to the field of biology, in particular to a method for preparing antimicrobial peptides using fruit flies. Background technique [0002] Antimicrobial peptides are antibacterial substances produced by insects in a short period of time after they are infiltrated with microorganisms and other factors. Antimicrobial peptides have small molecular weight, thermal stability, good water solubility, broad-spectrum antibacterial properties, and completely different antibacterial mechanisms from antibiotics. They have become the target of research and development of new antibacterial and anticancer drugs and have broad development prospects. [0003] Drosophila melanogaster belongs to the order Diptera, the family Drosophila, and the genus Drosophila. It is small in size, and the adult fly is about 0.5 cm long. Male Drosophila adults have 7 rings on their abdomen and have sexual combs. Adult female Drosophila has 5 rings on the abdomen an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18
Inventor 后嘉毅
Owner SHANGHAI HIGH SCHOOL
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