Method for preparing soybean isoflavone glycoside from soybean isoflavones aglycone

A technology for isoflavone aglycone and isoflavone glycosides, which is applied in the directions of hydrolase, fermentation, etc., can solve the problems of low cost, complicated operation and low enzyme activity, and achieves the effects of high conversion rate, simple extraction and low cost.

Inactive Publication Date: 2009-01-21
DALIAN POLYTECHNIC UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The production of soybean isoflavone glucosidase by microbial fermentation is relati

Method used

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  • Method for preparing soybean isoflavone glycoside from soybean isoflavones aglycone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] a. Chicken gold powder mixed with 0.2M / L, pH 6.0 phosphate buffer at a mass ratio of 1:5, extracted at 4°C for 24 hours, centrifuged to discard the residue, and ammonium sulfate was added to the supernatant to saturation to precipitate the enzyme Protein, collect protein, dissolve in 0.2M / L, pH 6.0 phosphate buffer, dialyze with cellophane bag to remove ammonium sulfate, centrifuge and discard the residue, which is the enzyme solution.

[0037] b. Mix 100 mL of daidzin with a concentration of 10 g / L and 100 mL of the enzyme solution in a. and react at a temperature of 25° C. for 30 hours.

[0038] c. Add 2 times the reaction volume of ethyl acetate to the reaction mixture, fully shake and extract, and let stand to separate layers. The conversion rate of daidzin into daidzein in the reaction product is 97.1%.

Embodiment 2

[0040] a. Chicken gold powder and 0.2M, pH 5.0 acetic acid buffer are mixed at a mass ratio of 1:6, extracted at 4°C for 3 hours, centrifuged to discard the residue, and 3 times the volume of 95% ethanol is added to the supernatant to precipitate the enzyme Protein, the supernatant was discarded by centrifugation, the protein was collected, and dissolved in acetate buffer (0.2M, pH5.0), which was the enzyme solution.

[0041] b. Mix 100 mL of 40 g / L genistin in acetate buffer solution (0.2 M, pH 5.0) and 200 mL of the enzyme solution in a., and react at a temperature of 40° C. for 30 hours.

[0042] c. Add 3 times the reaction volume of ethyl acetate to the reaction mixture, fully oscillate for extraction, and let stand to separate layers. The conversion rate of genistin into genistein in the reaction product was 90.4%.

Embodiment 3

[0044] a. Chicken gold powder and 0.2M, pH6.0 acetic acid buffer are mixed according to the mass ratio of 1:10, extracted at 4°C for 36 hours, centrifuged to discard the residue, and 6 times the volume of 95% ethanol is added to the supernatant to precipitate the enzyme Protein, the supernatant was discarded by centrifugation, the protein was collected, and dissolved in acetate buffer (0.2M, pH7.0), which was the enzyme solution.

[0045] b. Mix 70g / L genistin, 100mL acetate buffer solution (0.2M, pH7.0) and 200mL of the enzyme solution in a., and react at a temperature of 40°C for 18 hours.

[0046] c. Add 3 times the reaction volume of ethyl acetate to the reaction mixture, fully oscillate for extraction, and let stand to separate layers. The conversion rate of genistin into genistein in the reaction product was 93.6%.

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Abstract

The invention relates to a method for preparing aglycone from soybean isoflavone glucoside. The method comprises the following steps: galli stomachichum corium powder is mixed with 0.2 milligram per liter phosphoric acid buffer solution or acetic acid buffer solution with a pH value between 3.0 and 7.0 according to the mass ratio of 1 to 3-10; the mixture is leached for 3 to 36 hours at a temperature of between 4 and 40 DEG C and then waste slag is centrifuged; supernatant is added with ammonia sulfate or ethanol, and precipitated galli stomachichum corium zymoprotein is dissolved into the 0.2 milligram per liter phosphoric acid buffer solution or the acetic acid buffer solution with a pH value between 3.0 and 7.0, and then galli stomachichum corium enzyme solution is obtained; enzyme, the soybean isoflavone glucoside and the buffer solution are mixed and reacted for 3 to 30 hours at a temperature of between 10 and 50 DEG C and with a pH value between 3.0 and 7.0; and the soybean isoflavone aglycone is extracted. The method overcomes the defects of large destructiveness, large pollution and poor purposiveness of the acid and alkali hydrolysis method on the aglycone and simultaneously overcomes the defect that the enzyme activity of the microbial enzyme hydrolysis method is generally low. The method has easy, simple and convenient technology, complete zymohydrolysis, high conversion and high edible safety, and is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for preparing soybean isoflavone aglycone, in particular to a method for extracting gallinaceous enzyme from gallinacea in the dry gizzard inner membrane of a pheasant family chicken, and using gallinacein to hydrolyze soybean isoflavone aglycon A method for preparing flavonoid glycosides to their aglycones. Background technique [0002] Soybean isoflavones are non-steroidal compounds containing aromatic rings. So far, 12 soybean isoflavone isomers have been isolated from soybeans, which are divided into two types: bound glycosides and free aglycones. The bound glycosides mainly include Nine forms of genistin, daidzin and daidzein; aglycones include three forms of genistein, daidzein and glycitein. These 12 soybean isoflavone isomers have different names because of the different groups at the specific positions of the mother ring. Genistin, daidzin and daidzein mentioned below in the present invention are all one of ...

Claims

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Application Information

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IPC IPC(8): C12P17/06C12N9/24
Inventor 芦明春康少华刘英新刘波金凤燮
Owner DALIAN POLYTECHNIC UNIVERSITY
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