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Method for complexation purification by liquid extraction of chlorogenic acid

A chlorogenic acid and complexing technology, which is applied in chemical instruments and methods, preparation of organic compounds, solvent extraction of liquid solutions, etc., can solve problems such as the method of chlorogenic acid that has not been reported, and can speed up the decomplexation reaction rate. and stripping rate, increase separation factor, and facilitate the effect of control and management

Inactive Publication Date: 2011-08-24
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Up to now, the method of separating chlorogenic acid by complex extraction method has not been reported at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035]The deoiled honeysuckle water extract with a pH value of 2 and a chlorogenic acid concentration of 0.5 g / L was used as the raw material, tributyl phosphate was used as the complexing agent, butyl acetate was used as the cosolvent, and aviation kerosene was used as the diluent. The volume ratio of solvent, co-solvent and diluent is 1:1:18 to prepare complex extractant, the raw material liquid and complex extractant are mixed and oscillated in a separating funnel at a volume ratio of 5:1, and the extraction temperature is 5°C , after being balanced, the layers are statically separated to obtain the upper organic phase with chlorogenic acid extracted.

[0036] After the above organic phase was washed 3 times with an aqueous hydrochloric acid solution with a pH value of 2, it was mixed with an aqueous sodium hydroxide solution with a pH value of 11 at a volume ratio of 1:5 in a separatory funnel, and the temperature was 35° C., and the number of stripping times was 5 times, ...

Embodiment 2

[0039] The water extract of Eucommia ulmoides leaves after deoiling with a pH value of 1 and a concentration of chlorogenic acid of 20g / L is used as a raw material, trioctyl phosphate is used as a complexing agent, methyl isobutyl ketone is used as a cosolvent, and diesel oil is used as a diluent. The volume ratio of complexing agent, co-solvent and diluent is 1:1:15 to prepare a complexing extractant, and the raw material liquid and complexing extractant are added into a constant interface pool at a volume ratio of 15:1 and stirred, and the extraction temperature is 25°C , after being balanced, the layers are statically separated to obtain the upper organic phase with chlorogenic acid extracted.

[0040] After washing the above-mentioned organic phase twice with an aqueous solution of hydrochloric acid with a pH value of 1, add an aqueous solution of sodium carbonate with a pH value of 8 at a volume ratio of 1:20 and stir in the constant interface tank, the temperature is 65° ...

Embodiment 3

[0043] The water extract after deoiling tobacco with pH value of 5 and chlorogenic acid concentration of 5g / L is used as raw material, trialkylphosphine oxide as complexing agent, odorless kerosene as diluent, and the volume ratio of complexing agent and diluent Prepare a complex extractant at a ratio of 2:5, pass the raw material solution and the complex extractant through the extraction tower countercurrently at a volume ratio of 10:1, the extraction temperature is 15°C, wait for equilibrium and then separate into layers to obtain chlorogenic acid. upper organic phase.

[0044] After washing the above organic phase with aqueous hydrochloric acid solution with a pH value of 5 for 6 times, add an aqueous solution of sodium bicarbonate with a pH value of 6.5 at a volume ratio of 1:10 and stir in a constant interface tank at a temperature of 25°C, and the number of back extractions is 8 times , combined the strips, adjusted the pH of the strips to 3 with hydrochloric acid, and s...

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Abstract

The present invention relates to a method of purifying chlorogenic acid with the complexation extraction technology. A complexation extractant used as a light phase for extraction contacts with plant extract used as a heavy phase to perform complexation and extraction; and the organic phase with the chlorogenic acid is stripped to prepare high-purity chlorogenic acid. The present invention is suitable of low-concentration plant extract; when the separation process of complexing and extracting the chlorogenic acid is used, the production cost can be decreased, the organic solvent residue can be reduced, the environmental pollution is slowed, and the present invention fits requirements of the green chemical technology.

Description

technical field [0001] The invention relates to a method for complex extraction and purification of chlorogenic acid, which belongs to the field of natural product purification. Background technique [0002] Chlorogenic acid is a kind of polyphenolic compound, which is a kind of biologically active substance widely existing in nature. The main ingredient of traditional Chinese medicine for clearing heat, detoxifying, anti-inflammatory and antibacterial. Studies have shown that chlorogenic acid has pharmacological effects such as antibacterial, antiviral, antioxidative, free radical scavenging, immune regulation, antitumor, and antilipidemia. Therefore, chlorogenic acid is more and more widely used in the fields of biochemical reagents, pharmaceutical intermediates and preparations, and is currently internationally recognized as "plant gold". Since chlorogenic acid often exists together with its isomers or analogues in nature, and their physical and chemical properties are ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C69/732C07C67/58B01D11/04
Inventor 卢定强王俊赵辉蒋奔凌岫泉邵林刘建欧阳平凯
Owner NANJING TECH UNIV
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