The invention discloses a preparation method of a moss-proof baking-free brick. The preparation method comprises the following operation steps: (1) uniformly mixing stainless steel slag, alunite and sal ammoniac, adding the mixture into a crusher for crushing, adding treating fluid into the mixture, and uniformly mixing and stirring the mixture to obtain a base material for later use; (2) uniformly mixing the base material, cement and fly ash, adding the mixture into a cement mortar mixer, carrying out stirring, adding water, a water reducing agent and an early strength agent into the mixer, continuing stirring the materials to obtain a mixture, adding the mixture into a mold, and carrying out compression molding to obtain bricks; and (3) taking out the bricks, and curing the bricks at room temperature for 15-20 days to obtain baking-free bricks. The moss-proof baking-free brick provided by the invention has excellent mechanical properties and high strength, is not easy to break duringcarrying and transportation, especially has excellent moss-proof properties, and can effectively prevent mosses and other bryophytes from growing and propagating on the surface of the brick.