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Method for producing biological organic chromium and special bacterial strain thereof

A technology of organic chromium and chromium ions, which is applied in the field of producing biological organic chromium, can solve problems such as low cost, and achieve the effects of low cost, high output and simple test conditions.

Active Publication Date: 2009-03-11
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of studies have shown that: various forms of mutagenesis can improve the ability of yeast strains to enrich chromium to varying degrees; the basic medium commonly used in fermentation is wort medium, which is relatively low in cost, and some yeast can be appropriately added to it Other nutrients required for growth, such as urea, corn steep liquor, etc.; the effect of adding chromium trichloride as a chromium source in the medium is better; Adding an appropriate amount of metal elements such as erbium and lanthanum to the medium can also improve the chromium-enriching effect to varying degrees, but rare earth elements are harmful to humans, so they should not be used in the production of GTF

Method used

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  • Method for producing biological organic chromium and special bacterial strain thereof
  • Method for producing biological organic chromium and special bacterial strain thereof
  • Method for producing biological organic chromium and special bacterial strain thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1, the acquisition and identification of Saccharomyces cerevisiae YSI-3.7

[0021] 1. The preparation of culture medium

[0022] Seed medium: 10g yeast extract powder, 20g peptone, 20g glucose, 1000ml distilled water, pH5.6.

[0023] Fermentation medium: chromium trichloride 1.56×10 -3 mol / L, yeast extract powder 10 g / L, peptone 20 g / L, glucose 20 g / L, solvent is water; pH is 5.6.

[0024] 2. Obtaining of strain YSI-3.7

[0025] 1. Obtainment of strain Y-3

[0026] Strain Y-3 was isolated from the yeast mud provided by Beijing Brewery. The specific steps are as follows:

[0027] (1) Sampling: Aseptically sample about 1 gram from fresh beer yeast mud, put it into sterilized physiological saline, and mix well.

[0028] (2) Dilution: Repeatedly dilute the sample solution with sterile saline to 10 -6 .

[0029] (3) Invert the plate: Take 1 ml of the dilution from step (2) and add it to a petri dish, pour the melted YEPD solid medium at 45°C, shake slowly to m...

Embodiment 2

[0073] Embodiment 2, utilize Saccharomyces cerevisiae YSI-3.7 to produce organic chromium

[0074] 1. The preparation of culture medium

[0075] Seed medium: 10g yeast extract powder, 20g peptone, 20g glucose, 1000ml distilled water, pH5.6.

[0076] Fermentation medium: chromium trichloride 1.13×10 -3 mol / L, yeast extract powder 5 g / L, peptone 15 g / L, glucose 15 g / L, solvent is water; the pH of the medium is 5.0.

[0077] 2. Production of organic chromium

[0078] Pick 1 ring of activated Saccharomyces cerevisiae YSI-3.7 with an inoculation loop and place it in the seed medium, and cultivate it on a shaker at 28°C, 200r / m, and 12mm radius of rotation for 15h to obtain the seed liquid; T) Inoculate the seed solution into the fermentation medium, and ferment for 25 hours on a shaker at 25°C, 150r / m, and 12mm radius of rotation. The experiment was repeated three times, and the results were mean ± standard deviation.

[0079] After the fermentation was finished, the biomass a...

Embodiment 3

[0080] Embodiment 3, utilize Saccharomyces cerevisiae YSI-3.7 to produce organic chromium

[0081] 1. The preparation of culture medium

[0082] Seed medium: 10g yeast extract powder, 20g peptone, 20g glucose, 1000ml distilled water, pH5.6.

[0083] Fermentation medium: chromium trichloride 1.88×10 -3 mol / liter, 15 grams / liter of yeast extract powder, 25 grams / liter of peptone, 25 grams / liter of glucose, and the solvent is water; the pH of the medium is 6.0.

[0084] 2. Production of organic chromium

[0085] Pick 1 ring of activated Saccharomyces cerevisiae YSI-3.7 with an inoculation loop and place it in the seed medium, and cultivate it on a shaker at 28°C, 200r / m, and 12mm radius of rotation for 15h to obtain the seed liquid; T) Inoculate the seed solution into the fermentation medium, and ferment for 35 hours on a shaker at 31°C, 300r / m, and 12mm radius of rotation. The experiment was repeated three times, and the results were mean ± standard deviation.

[0086] Afte...

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Abstract

The invention discloses a method for producing bio-organic chrome and a special bacterial strain thereof. The bacterial strain provided by the invention is Saccharomyces cerevisiae YSI-3.7 CGMCC No.2687, which has the capability of producing organic chrome with high yield. The method for producing the organic chrome is to culture the Saccharomyces cerevisiae YSI-3.7 CGMCC No.2687 to produce the organic chrome. The organic chrome produced by the method has the advantages of simple testing condition, low cost and high yield. The method for producing bio-organic chrome and the special bacterial strain thereof have important economic significance and social significance.

Description

technical field [0001] The invention relates to a method for producing bioorganic chromium and a special bacterial strain thereof. Background technique [0002] GTF (Glucose Tolerance Factor) is a substance that can maintain normal blood glucose levels in animals. It can increase the glucose tolerance of animals and improve the activity and function of insulin, and promote the binding of insulin to cell membrane receptors. Stimulate the absorption of glucose by animal tissues. At present, there is no report on the extraction of completely purified GTF components. Toepfer et al. separated and purified chromium-rich yeast and speculated on the structure of GTF: GTF is composed of trivalent chromium ions and nicotinic acid, glycine, glutamic acid and A low-molecular-weight octahedral complex formed by cysteine; there is a characteristic absorption peak at 260nm; GTF is centered on trivalent chromium, and is trans-coordinated with two nicotinic acids with pyridine nitrogen, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P9/00C12R1/865
Inventor 吕加平刘鹭高艳红刘晓铭
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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