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Bio-sensing multilayer film of titanium oxide contained nanometer sheet and method for making same

A technology containing titanium oxide and nano-sheets, which is applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of harsh conditions and difficulty in controlling the thickness of myoglobin-intercalated titanium oxide nanocomposite films, etc., and achieve a large amount of fixation , to achieve rapid electrode reaction, reduce the effect of charge and ion transfer resistance

Inactive Publication Date: 2011-11-02
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the conditions for obtaining the myoglobin-intercalated titanium oxide structure are relatively harsh. In addition, it is not easy to control the thickness of the myoglobin-intercalated titanium oxide nanocomposite film by drip coating.

Method used

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  • Bio-sensing multilayer film of titanium oxide contained nanometer sheet and method for making same
  • Bio-sensing multilayer film of titanium oxide contained nanometer sheet and method for making same
  • Bio-sensing multilayer film of titanium oxide contained nanometer sheet and method for making same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A. Preparation of titanium oxide nanosheets: 3.26g (0.01mol) of Cs2CO3 and 4.16g (0.052mol) of TiO 2 Place in an agate mortar and grind quickly for 10 minutes; put the mixed powder into Al 2 o 3 Crucible, and then heated in a muffle furnace at a rate of 10 °C / min to 800 °C for 3 hours to remove CO 2 ; After cooling to room temperature, the powder was ground again with an agate mortar for 30 minutes, and calcined at 800°C for 30 hours. After cooling to room temperature, the grinding and calcination process was repeated to generate non-stoichiometric alkali metal titanate Cs x Ti 2-x / 4 □ x / 4 o 4 Solid powder (x is 0.7, □ is vacancy). Weigh 2.0g of alkali metal titanate solid powder and disperse it into 200mL of 1mol / L HCl solution, stir for 15 hours and then filter with suction, repeat 3 times, wash the solid precipitate with distilled water until the pH value is 7.0, and the proton titanate can be obtained h x Ti 2-x / 4 □ x / 4 o 4 ·H 2 O(x is 0.7, □ is vacancy)...

Embodiment 2

[0036] A. The preparation method of titanium oxide nanosheets is the same as in Example 1.

[0037] B. (Titanium oxide nanosheets / myoglobin) 5 Preparation of multilayer film: clean the glassy carbon electrode, and then use N 2 Blow dry; immerse the electrode in 20g / L polydiallylammonium chloride aqueous solution for 10 minutes, take it out, rinse with distilled water, and use N 2 Blow dry; immerse the treated electrode in a concentration of 0.5g / L titanium oxide nanosheet sol for 10 minutes, take it out, rinse with distilled water, and wash with N 2 Blow dry, then immerse the electrode in the myoglobin solution with a concentration of 2.0g / L for 10 minutes, take it out, rinse with distilled water, and rinse with N 2 Blow dry to complete the preparation of a (titanium oxide nanosheet / myoglobin) film; repeat the above steps to prepare (titanium oxide nanosheet / myoglobin) 5 multilayer film. Using Shimadzu UV-2501PC ultraviolet-visible spectrophotometer to detect the assembly ...

Embodiment 3

[0040] A. Preparation of titanium oxide nanosheets: 3.26g (0.01mol) of Cs2CO3 and 4.40g (0.055mol) of TiO 2 Put into an agate mortar and grind quickly for 30 minutes; put the mixed powder into Al 2 o 3 Crucible, and then heated in a muffle furnace at a rate of 5 °C / min to 600 °C for 3 hours to remove CO 2 ; After natural cooling to room temperature, the powder was ground again with an agate mortar for 20 minutes, and calcined at 700°C for 20 hours. After cooling to room temperature, the grinding and calcination process was repeated to generate non-stoichiometric alkali metal titanate Cs x Ti 2-x / 4 □ x / 4 o 4(x is 0.67, □ is vacancy) solid powder. Weigh 2.0g of alkali metal titanate solid powder and disperse it into 240mL of 1mol / L HCl solution, stir for 24 hours and then filter with suction, repeat twice, wash the solid precipitate with distilled water until the pH value is 6.5, and you can get proton titanate h x Ti 2-x / 4 □ x / 4 o 4 ·H 2 O(x is 0.67, □ is vacancy). ...

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Abstract

The invention relates to a bio-sensitive multilayer film containing a titanium oxide nano-plate and a preparation method thereof, which belongs to the technical field of electrochemical biosensor and preparation thereof. The bio-sensitive multilayer film is formed by superposing titanium oxide nano-plate layers and biological active substance layers repeatedly in turn; the preparation method comprises the following steps: preparing the titanium oxide nano-plate first, and then assembling the titanium oxide nano-plate layers and the biological active substance layers on the surface of a glass-carbon electrode in turn through the static action between the titanium oxide nano-plate and the biological active substance with opposite charges. Immobilization of various biological active substances can be realized by using the bio-sensitive multilayer film which is prepared by a layer upon layer assembling method and contains the titanium oxide nano-plate and the biological active substances have larger fixed quantity; thickness regulation and control of the multilayer film can be realized through controlling the assembled layer number, so the method is favorable for reducing charge and ion transfer resistance, and realizes quick electrode reaction; and the glass-carbon electrode modified by the bio-sensitive multilayer film has sensitive signal response and high efficient catalytic ability.

Description

technical field [0001] The invention belongs to the technical field of electrochemical biosensors and preparation thereof, in particular to a biosensitive multilayer film containing titanium oxide nanosheets and a preparation method thereof. Background technique [0002] Electrochemical biosensors have the characteristics of stable performance, high selectivity, low price, easy operation, and easy miniaturization, and have been widely used in clinical diagnosis, industrial control, food testing, drug analysis, environmental analysis, and biochips. Among them, the third-generation reagent-free electrochemical biosensors, which use direct electrochemical (ie, direct electron transfer) detection between biologically active substances and electrodes, have attracted more attention and are currently a hot spot in the field of electrochemical biosensor research. However, the sensitivity and application range of reagent-free electrochemical biosensors still have certain limitations,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
Inventor 杨文胜谢青张亚辉
Owner BEIJING UNIV OF CHEM TECH