Process for producing fluorescent composite microgel hypersensitive to temperature and pH

Abstract

The invention discloses a preparation method of fluorescent complex microgel which is sensitive to temperature and pH. The method comprises the technical steps of preparing a mixed surface active agent, preparing the oil phase of a template, preparing an emulsive liquid, preparing a water phase, preparing N-isopropyl acryl amide copolymerized methacrylic acid microgel, preparing turgid N-isopropyl acryl amide copolymerized methacrylic acid microgel, preparing a mixed liquid of Gamma-aminopropyl triethoxy silane and normal heptane, preparing a silicon dioxide polymer complex microballoon decorated by a surface deposited amino group, preparing a mixed liquid of fluorescein isothiocyanate and absolute ethyl alcohol as well as preparing the fluorescent complex microgel. In the invention, the fluorescein isothiocyanate with high quantum yield and good optical stability is adopted as fluorescent matter; the prepared fluorescent complex microgel is sensitive to temperature and pH; and the size of the prepared fluorescent complex microgel is in a micron range and is hard to be aggregated. The method has the advantages of reasonable design, practical technique and easy operation; moreover, reactions are carried out under normal temperature and the like; the method can be applied to the fields of controlled release of medicament, biological probe, chemical separation, and the like.

Description

technical field

[0001] The invention belongs to the technical field of materials, and specifically relates to colloid chemistry, for example: preparation of colloid materials or solutions thereof not included in other classes; manufacture of microcapsules or microspheres. Background technique

[0002] The physical-chemical properties and colloidal properties of environment-sensitive microgels will show corresponding stimuli responsiveness with changes in external environmental conditions (such as temperature, pH, light, ionic strength, electric field or magnetic field, etc.). called smart microgels. Due to the unique response properties of smart microgels to the external environment, more and more scientists have paid attention to them. series of research results. Among them, the research on temperature-sensitive microgel based on poly-N-isopropylacrylamide (PNIPAM) is particularly eye-catching. Its critical phase transition temperature is around 32°C. When the temperature...

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