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Method for increasing cordycepin content in Cordceps militaris solid culture medium

A solid medium, cordycepin technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effect of improving efficiency and increasing content

Inactive Publication Date: 2009-05-27
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current patents on the solid fermentation of Cordyceps militaris mainly focus on the research on the fruiting body production process, and there are few reports on the large-scale production of cordycepin by solid medium

Method used

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  • Method for increasing cordycepin content in Cordceps militaris solid culture medium

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Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1: in 10 volumes, be 350ml, internal diameter be 6.2cm, height be that 20 purpose rices and 14 purpose rice combinations (according to the mass ratio proportioning of 1: 2) of packing into respectively in 15 cm cylindrical colorless glass bottles, Add 32mL nutrient solution (3% glucose, 3.5% peptone, K 2 HPO 4 0.05%, MgSO 4 ·7H 2 O 0.05%, VB 1 (2mg / L), water 1000mL, nutrient solution formulated with a natural pH formula), sealed with a high-temperature resistant single-layer polypropylene plastic film, sterilized by autoclaving at 121°C for 30 minutes, and inoculated with Cordyceps militaris (CGMCCNo.2459) after cooling. The liquid mother species was cultured at 25°C in the dark for 10 days. From the 11th day onwards, the scattered light should not be less than 10 hours per day, the temperature should be kept at 25°C, and the relative humidity should be increased to about 80%. , the temperature is kept at about 22°C, and the air humidity is about 85%. Af...

Embodiment 2

[0030] Embodiment 2: in 10 volumes, be 350ml, internal diameter be 6.2cm, height be to pack 20 order rice and 14 order rice combinations (according to the mass ratio proportioning of 1: 2) respectively in the cylindrical colorless glass bottle of 15cm, Add 40mL nutrient solution (3% glucose, 3.5% peptone, K 2 HPO 4 0.05%, MgSO 4 ·7H 2 O 0.05%, VB 1(2mg / L), water 1000mL, nutrient solution formulated with a natural pH formula), sealed with a high-temperature resistant single-layer polypropylene plastic film, sterilized by autoclaving at 121°C for 30 minutes, and inoculated with Cordyceps militaris (CGMCCNo.2459) after cooling. The liquid mother species was cultured at 25°C in the dark for 10 days. From the 11th day onwards, the scattered light should not be less than 10 hours per day, the temperature should be kept at 25°C, and the relative humidity should be increased to about 80%. , the temperature is maintained at around 22°C, and the air humidity is around 85%. After ...

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Abstract

The invention provides a method for increasing cordycepin content in Chinese caterpillar fungus solid medium, which comprises three steps of activation of fungus, preparation of liquid fungus and culture of the Chinese caterpillar fungus. The method is characterized in that in the method, rice with the grain size of 20 meshes and 14 meshes is mixed according to the mass ratio of 1:2 to obtain culture medium; 3 percent of glucose, 3.5 percent of peptone, 0.05 percent of K2HPO4, 0.05 percent of MgSO4.7H2O, 2mg / L VB1 and 1,000mL water are used to prepare nutrient solution, and the Chinese caterpillar fungus is cultured according to the prior solid fermentation method; and after stroma of the Chinese caterpillar fungus grows out, illumination intensity and time are increased. The method has the advantages of greatly increasing the cordycepin content (more than 0.76 percent) in the culture medium and having important significance on improving yield and efficiency of producing the cordycepin by solid fermentation of the Chinese caterpillar fungus, along with simple and quick operation; therefore, the method is suitable for mass production of the cordycepin by the solid fermentation of the Chinese caterpillar fungus.

Description

technical field [0001] The invention relates to fungal fermentation engineering, in particular to solid culture of Cordyceps militaris and high-yield medicinal raw material cordycepin. Background technique [0002] The molecular formula of cordycepin is C 10 h 13 N 5 o 3 , Molecular weight 251, alkaline, needle-like or flaky crystal, melting point 230°C-231°C, soluble in water, hot ethanol and methanol, insoluble in benzene, ether and chloroform, positive for anthrone, the maximum absorption wavelength of ultraviolet light is 259nm . The structural formula of cordycepin is as follows: [0003] [0004] Cordycepin produced by Cordyceps militaris has a variety of biological activities, such as: antibacterial effect, anti-tumor effect, inhibitory effect of human immunodeficiency virus HIVI virus, weight loss effect, immune regulation effect and obvious hypoglycemic and blood fat-lowering effect. Among them, the effect of lowering blood lipid is particularly obvious, wh...

Claims

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Application Information

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IPC IPC(8): C12P19/40C12R1/79
Inventor 康冀川文庭池雷帮星何劲
Owner GUIZHOU UNIV
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