Scaffold

A scaffold material and fiber technology, applied in cell culture supports/coatings, methods of supporting/immobilizing microorganisms, biochemical instruments, etc., can solve problems such as fiber accumulation density changes, uneven packing density, and difficulty in maintaining potential differences

Inactive Publication Date: 2009-05-27
TEIJIN LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, the fibers are captured on the planar capture electrode, so when the fibers accumulate to a certain thickness or more, the electrodes are covered by the deposits, and it is difficult to maintain a certain potential difference, and the deposit density of the fibers changes in the accumulation direction.
In addition, the accumulation density will also be uneven in the direction perpendicular to the accumulation direction.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] (Preparation of spinning dope)

[0102] A lactic acid-glycolic acid copolymer (molar ratio = 50 / 50) was dissolved in a mixed solvent of methylene chloride and ethanol to prepare a 15% by weight spinning stock solution.

[0103] (spinning)

[0104] use Figure 4 In the shown device, a cylindrical roll comprising a fiber assembly is obtained by electrospinning. The inner diameter of the nozzle (1) is 1.3 mm. The distance from the nozzle (1) to the winding device (7) is 20 cm, and the distance from the nozzle (1) to the static elimination device (8) is 35 cm. The applied voltage was 15 kV. A winding device (7) and a static eliminator (8) manufactured by Kasuga Denki Co., Ltd. were installed between the nozzle (1) and the negative electrode (5). A positive electrode (4) is inserted into the spinning dope holding tank (3). The rotation speed of the winding device (7) was set at 100 rpm.

[0105] Put the spinning stock solution into the spinning stock solution holding ...

Embodiment 2

[0123] (Preparation of spinning dope)

[0124] It prepared using the lactic acid-glycolic acid copolymer similarly to Example 1 so that it might become 10 weight%.

[0125] (spinning)

[0126] A cylindrical roll including a fiber aggregate was obtained by the same method as in Example 1, except that the discharge time was 90 minutes and the heat treatment was performed at 70° C. for 10 minutes.

[0127] (Cut out)

[0128] Using the same method as in Example 1, cut out the corresponding Figure 5 10 cylindrical stent materials of diameter 5mm x height 5mm. will be parallel to the corresponding Figure 5 The micrograph (magnification 15 times) of the section of the bottom surface of the scaffold material of 10 is shown in Figure 6 (top bottom) and Figure 7 (lower bottom). It can be seen that the fibers are accumulated in layers.

[0129] (characteristic evaluation)

[0130] The characteristics were measured by the same method as in Example 1. The results are shown in...

Embodiment 3

[0145] (manufacturing of bracket materials)

[0146] Repeat the same operation as in Example 2 to obtain the corresponding Figure 5 12 cylindrical stent materials with a diameter of 5 mm x a height of 5 mm. Table 1 shows the results of measuring the properties.

[0147] will be perpendicular to the corresponding Figure 5 The micrograph (magnification 200 times) of the section of the bottom surface of the scaffold material of 12 is shown in Figure 10 . The fibers are oriented in the plane direction. Additionally, the parallel to the corresponding Figure 5 The micrograph (magnification 15 times) of the section of the bottom surface of the scaffold material of (12) is shown in Figure 11 (top bottom) and Figure 12 (lower bottom). It can be seen that the fibers are densely packed like a mesh.

[0148] (biological evaluation)

[0149] Using the obtained scaffold material, cells were cultured by the same method as in Example 2, and the amount of DNA was measured. The...

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Abstract

The object is to provide a scaffold material which has excellent mechanical strength and an excellent capability of growing a cell and is suitable as a cell culture substrate or a prosthesis. Disclosed is a scaffold material for growing a cell. The scaffold material comprises an aggregate of fibers, has a three-dimensional structure composed of two bottom faces and side faces, and satisfies the following requirements (1) to (4): (1) the fibers are oriented in the side-wise direction; (2) each of the fibers has a diameter of 0.05 to 50 [mu]m; (3) each of the fibers is composed mainly of a biocompatible polymer; and (4) the scaffold material has an apparent density of 95 to 350 kg / m<3>.

Description

technical field [0001] The present invention relates to scaffold materials used for cell proliferation. The present invention relates to scaffold materials comprising fibrous assemblies, suitable repair materials or cell culture substrates. Background technique [0002] In recent years, as a treatment method for severely damaged biological tissues, regenerative medical research has been actively carried out in which the original biological tissues are reconstructed using the differentiation and proliferation capabilities of cells. When cells in a living body differentiate and proliferate, the extracellular matrix functions as a scaffold to construct tissues. However, when the tissue is severely damaged, it is necessary to supplement it with artificial and natural materials before the cells themselves produce the matrix. That is, the scaffold material (restoration material) is an important factor for creating an optimal environment in terms of tissue construction. Examples...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/00C12M3/00C12N5/06D04H1/72D04H3/07
CPCA61L27/58D01D5/0007D01F6/22A61L27/18D01F6/66C12N5/0068D04H3/07A61L27/14C12N2533/40D01F6/04A61L27/56C12N2533/30A61L27/50C12M25/14C08L67/04C12N11/04C12N5/06
Inventor 福富千秋兼子博章北园英一茅岛美佳中山三由纪
Owner TEIJIN LTD
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