The invention discloses a preparation method for the self-assembly of electrospinning rosary-like fibers, and belongs to the technical field of chemistry and material. By inspired by the situation that a cobweb can be assembled to a spindle structure in the wet environment respectively, a static electricity spinning device is utilized to prepare man-made fibers, and an aluminum bracket is used for receiving, so as to form the man-made fibers with the structure that nano-particles are adhered to surfaces; the man-made fibers are allowed to be assembled to the micron-sized ' rosary' structure respectively in the wet environment. According to the preparation method provided by the invention, the manufacturing process is simpler, the cost is lower, the operability is strong, and the method is suitable for mass productions; the respectively assembled fibers have stable quality and long practicability; the method can be used for collecting fresh water, reducing fog, solving the problem of serious water shortage in deserts and wastelands, and dispersing the steam around airports, street lamps, and signal lamps.