Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Gelatin/calcium phosphate composite porous microsphere for bone repair and preparation method and usage of the porous microsphere

A technology of porous microspheres and calcium phosphate, which is applied in the field of preparation of gelatin derivative materials, achieves good biocompatibility, induces osteogenic activity, promotes differentiation of bone marrow stromal cells, and has wide application value.

Active Publication Date: 2012-11-14
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention prepares composite porous microspheres by co-precipitating soluble calcium salts and phosphorus salts in gelatin emulsion, adding appropriate amount of velvet antler polypeptide or bone morphogenetic protein in the reaction to obtain bone repair composite material and preparation of the composite material The method has not been reported in the literature

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Accurately weigh 1 gram of gelatin powder and put it into a 100ml beaker, add 5ml of deionized water, put it in a water bath at 50±1°C and stir to dissolve it, then add 0.0025mol required for the formation of calcium phosphate into the beaker Calcium nitrate solid, and then add velvet antler polypeptide 3mg to make a mixed solution.

[0034] 2. Under stirring at a rotating speed of 500 rpm, slowly add the above solution dropwise to 50ml of soybean oil phase dissolved in a surfactant (SPAN80, 1g / 50ml) to form a water-in-oil emulsion of the gelatin solution, and react Keep the temperature at 45±1°C, and continue to stir for 2 hours, then add 5% Na 2 HPO 4 solution (Ca / P molar ratio is 5:3).

[0035] 3. After the reaction, the reactant at 45±1°C was quickly transferred to an ice-water bath, kept stirring, and cooled for 40 minutes, so that the gelatin microspheres containing calcium phosphate were gelled, and calcium was uniformly dispersed in the gelatin microspheres. ...

Embodiment 2

[0040] 1. Accurately weigh 1 gram of gelatin powder and put it into a 100ml beaker, add 5ml of deionized water, put it in a water bath at 50±1°C and stir to dissolve it, then add 0.003mol required for the formation of calcium phosphate into the beaker Calcium chloride solid, and then add 20mg deer antler polypeptide to it to form a mixed solution.

[0041] 2. Slowly add the above solution dropwise to 100ml of olive oil phase dissolved in surfactant (SPAN 60-1g / 50ml) under stirring at a speed of 500 rpm to form a water-in-oil emulsion of gelatin solution , the reaction temperature was kept at 45±1°C, and continued to stir for 2 hours, and then added dropwise to the emulsion system with a mass concentration of 10% Na 2 HPO 4 solution (Ca / P molar ratio is 5:3).

[0042] 3. After the reaction, the reactant at 45°C ± 1°C was quickly transferred to an ice-water bath, kept stirring, and cooled for 40 minutes, so that the gelatin microspheres containing calcium phosphate were gelled...

Embodiment 3

[0047] 1. Accurately weigh 1 gram of gelatin powder and put it into a 100ml beaker, add 5ml of deionized water, put it in a water bath at 40±1°C and stir to dissolve it, then add 0.01mol of calcium phosphate required to form calcium phosphate into the beaker Calcium chloride solid is formulated into a gelatin solution containing a certain concentration of calcium ions, and then 20 mg of pilose antler polypeptide is added thereto and dispersed evenly.

[0048] 2. Under stirring at a speed of 200 rpm, slowly add the above solution dropwise to 75ml of the camellia oil phase in which a surfactant (dioctyl sodium sulfosuccinate (AOT)-1g / 50ml) is dissolved, Form a water-in-oil emulsion of a gelatin solution, keep the reaction temperature at 45±1°C, and continue to stir for 2 hours, then add dropwise a mass concentration of 10% Na 2 HPO 4 solution (Ca / P molar ratio 1.5:1).

[0049] 3. After the reaction, the reactant at 45±1°C was quickly transferred to an ice-water bath, kept stir...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of the preparation of gelatin derivative materials and in particular relates to a composite porous microsphere material formed by compounding gelatin, calcium phosphate and an antler polypeptide bioactive tissue or bone morphogenic protein (BMP). The composite porous microsphere is formed by uniformly dispersing, in a gelatin composite porous microsphere, the calcium phosphate and active-substance antler polypeptide or bone morphogenic protein which is capable of promoting bone growth; wherein, the weight of the active-substance antler polypeptide or the bone morphogenic protein accounts for millesimal to five percent of the weight of the gelatin; soluble calcium salt which forms the calcium phosphate ranges from 0.0003 to 0.03 mol in each gelatin; and the amount of soluble phosphate causes the ratio between Ca element and P element to range from 1:1 to 2:1. The composite porous microsphere material can also contain crosslinking agent. The composite porous microsphere material is a bone repair biological material which has good biocompatibility and good bone induction activity.

Description

technical field [0001] The invention belongs to the field of preparation of gelatin derivative materials, in particular to a composite porous microsphere material synthesized from gelatin, calcium phosphate and a bioactive tissue of pilose antler polypeptide or bone morphogenetic protein (BMP). Biocompatibility and osteogenic activity of biomaterials for bone repair. Background technique [0002] Gelatin is the product of collagen degradation. It not only maintains the unique advantages of collagen as a biological material, but also has no antigenicity compared with collagen, and there is no rejection when implanted in the body; gelatin is cheap and easy to obtain; Soluble in water; the detection methods of its physical and chemical properties have been developed relatively mature and standardized. Therefore, the composite material of gelatin and calcium phosphate is also a hotspot in the research of bone repair materials at present. [0003] Traditional gelatin and calciu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/54A61L27/46
Inventor 陈丽娟郭燕川孙瑞雪史京京邓旭明宋宇
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com