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Bio-derivative tendon repair material and preparation method thereof

A technology for repairing materials and tendons, applied in the direction of muscle, medical science, ligament, etc., to achieve the effect of promoting construction, easy ingrowth, and promoting healing and regeneration

Active Publication Date: 2012-08-15
北京科健生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gao Xinsheng and others believed that after transplantation of ultra-low temperature cryopreserved tendon tissue, although it lost its metabolic function, it maintained the continuity of tendon tissue and laid the structural foundation for tendon revascularization and living cells; their experiments observed that tendon tissue After transplantation, a large number of newly formed fibroblasts, connective tissue cells and capillaries soak into the surface of the grafted tendon and surrounding tissues along the surface of the tendon at both ends and between the tendon bundles. The repair tends to mature over time, and finally evolves into a neatly arranged wavy pattern. Type collagen fiber bundles, so that the transplanted tendon segment becomes a tendon tissue with the metabolic function of living cells. As for the mechanism of the repair speed after tendon transplantation, further research is needed.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The fresh and qualified human tendon is removed from the accessory tissue, and the specimen is placed in a depyrogenated glass container and rinsed repeatedly with depyrogenated pure water to remove blood components; the material is washed with 1% H 2 o 2 Soak (1:10 volume), vibrate and shake in a water bath shaker at 37°C for 6 hours (40rpm), replace the liquid every 3 hours; rinse repeatedly with depyrogenated pure water for 1 hour, replace the liquid every 10 minutes; wash the material with methanol / chloroform ( v / v, 1 / 1) degrease (dosage 1:3 volume ratio), soak for 1 hour, change the liquid twice during the period, stir in the middle, until the solution is clear and free of grease; rinse with depyrogenated pure water until there is no organic solvent Residue, that is, no chloroform smell; digest the material with 0.25% trypsin for 6 hours, the dosage is 1:2 volume ratio; rinse repeatedly with depyrogenated pure water for 24 hours, and replace it several times in the...

Embodiment 2

[0037] The fresh and qualified human tendon material is removed from the accessory tissue, and the specimen is placed in a depyrogenated glass container and rinsed repeatedly with depyrogenated pure water to remove blood components; the material is sterilized with 75% alcohol for 20 minutes, and then 0.8% alcohol Sterilize in peracetic acid-10% ethanol mixed solution, the mass volume ratio of material and solution is 1:5, soak for 50 minutes, change the solution every 25 minutes, and stir midway. Rinse repeatedly with depyrogenated pure water for 1 hour, replace the liquid every 20 minutes; degrease the material with methanol / dichloromethane (v / v, 3 / 1) (volume ratio 1:3), soak for 1.5 hours, and replace the liquid 2 times, stir in the middle until the solution is clear and free of grease; rinse with depyrogenated pure water until no organic solvent remains, that is, no chloroform smell; digest the material with 0.18% trypsin for 8 hours, the dosage is 1:3 volume ratio; Rinse r...

Embodiment 3

[0039] The fresh bovine tendon material was removed from the accessory tissue, and the specimen was placed in a depyrogenated glass container and rinsed repeatedly with depyrogenated pure water to remove blood components; the material was washed with 2% H 2 o 2 Soak (1:8 volume), vibrate and shake in a water bath shaker at 37°C for 5 hours (40rpm), replace the liquid in the first 2 hours; rinse repeatedly with depyrogenated pure water for 1 hour, replace the liquid every 10 minutes; wash the material with methanol / chloroform ( v / v, 1 / 1) degrease (dosage 1:3 volume ratio), soak for 1 hour, change the solution twice, stir in the middle, until the solution is clear and free of grease; rinse with depyrogenated pure water until no organic solvent remains , that is, no chloroform smell; the material was digested with 0.5% trypsin for 3 hours, and the dosage was 1:2 volume ratio; the depyrogenated pure water was rinsed repeatedly for 24 hours, and replaced several times in the middle...

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PUM

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Abstract

The invention provides a repair material for biological derivative tendon. The material takes homogeneous or heterogeneous tendon as a raw material and is formed through disinfection, degreasing, deproteinization, decellularization, freeze drying, depolymerization, devillication recombination and secondary disinfection. A method for preparing a tendon scaffold material comprises the following steps that: on one hand, a periphery structure of a tendon tissue is destructed through treatment processes of removing soft tissues, the disinfecting, the degreasing and the decellularization, and the repellency of a transplantation receipt to the tendon is reduced; on the other hand, a basic mechanism, partial enzyme activity of the tendon tissue and the continuity of the tendon tissue are further maintained through a freeze drying process so as to provide a good scaffold structure for the revascularization and cell tissue of the tendon.

Description

technical field [0001] The invention relates to a biologically derived tendon repair material and a preparation method thereof, belonging to the field of medical materials. Background technique [0002] The repair of tendon defects is still dominated by autologous tendon transplantation, but the source of autologous tendon donors is limited. Although the clinical application of artificial tendon transplantation has been reported, the problems of its materials and the combination with the receptor have not been fully resolved. In recent years, domestic and foreign scholars have done a lot of research on tendon transplantation, analyzing and evaluating the experimental results and clinical application effects of tendon preparation, preservation and transplantation. [0003] At present, there are mainly three methods for the preparation and storage of tendon, namely ultra-low temperature frozen storage for standby, drug soaking and radiation irradiation. [0004] Ultra-low tem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61F2/08
Inventor 李次会
Owner 北京科健生物技术有限公司
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